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Bmkloud Gal

Wax soo wadaban

16s / 18s / eveickon drive-ngs

16s / 18s / eveickon seququing-ngs muuqaal muuqaal ah

Isugeynta filimka ifafaynta ee ifafaynta, si gaar ah u beegsanayso 16-ka, 18-ka, iyo calaamadaha hidda-wadaha, waa hab awood badan oo loogu tala galay in laga xiro phyyyEny, ee diinta canshuuraha, iyo noocyada badan ee bulshada microning. Qaabkan waxaa ku jira isku xirka gobollada loo dejiyo ee calaamadaha hidda-haynta ee guriga. Asal ahaan loo soo bandhigay sidii faro-koodhka molikalicWelles et alSanadkii 1977, farsamadan ayaa isbeddelay microbiome-ka kufsiga ah iyadoo awood u siineysa falanqaynta go'doominta. Iyada oo loo marayo isku xirka 16-ka (bakteeriyada), 18s (fangaska), iyo meel bannaan oo gudaha ah oo qoraal ah (fangas), cilmi baarayaashu ma ogaan karaan kaliya noocyo badan laakiin sidoo kale waa dhif iyo naadir iyo kuwa aan la garaneyn. Si ballaaran ayaa loo qaatay sidii aalad muhiim ah, isku xigxiga 'filinkon' wuxuu u noqday qalab ka kooban ka kooban midab kala duwan oo ku saabsan bey'adaha kaladuwan, oo ay kujirto afkii bini-aadamka, xiidmaha, saxarada, iyo wixii ka dambeeya.

Nala soo xiriir

Faahfaahinta adeegga

Bioinformatics

Natiijooyinka Demo

Daabacaadda Feejignaanta

Astaamaha adeegga

● Qalabka isku xirka: Iverina novaesaq.

Extred Cod-weyneynta Gobollada Gaaban ee 16s, 18s iyo bartilmaameedyada kale ee cod-bixinta.

● Xulasho dabacsan oo ku saabsan codicton.

● waayo-aragnimo mashruuc hore oo leh bartilmaameedyo cod dheer oo badan.

Faa'iidooyinka adeegga

●Go'doon la'aan:Aqoonsiga deg-degga ah ee ka kooban microbial oo ku saabsan saamiyada deegaanka.

●Xal u qaadista sare: Qeybaha badan ee muunadaha deegaanka ah.

●Si ballaaran loogu dabaqi karo: Daraasadaha bulshada kala duwan ee microbial.

●Falanqaynta Bioinformatic ee dhameystiran: Xidhmada ugu dambeysa ee Qiime2 (fahamka qiyaasaha ee loo yaqaan 'miclobight cilmiga' microbial ecology ') oo leh falanqeyn kala duwan marka loo eego keydka macluumaadka, faahfaahinta, OTU / ASV.

●Khibrad ballaaran: Iyada oo leh 150 kun oo mashruuc oo isbadal ah oo lagu qiyaasi karo sanadkiiba, BMKGE waxay keentaa toban sano oo waayo-aragnimo ah, oo ah koox aad u wanaagsan oo xirfad leh, oo ah ka kooban, iyo taageerada iibka ee ugu fiican.

Faahfaahinta adeegga

Maktabad	Istaraatiijiyadda isku xigta	Xogta lagu taliyay
Tallaaxid	NEMLENIA PE250	50/100 / 300k calaamadaha (akhri laba-labo)

Shuruudaha adeegga

U fiirsashada (NG / μL)	Wadarta lacagta (ng)	Mugga (μl)
≥1	≥200	≥20

● Ciidda / gogosha: 1-2G
● Xayawaanka ku dhaca xiidmaha: 0.5-2G
Tusmada cayayaanka-cayayaanka: 0.1-0.25g
● Dhulka dushiisa (wasakhda lagu saleeyay): 0.1-0.5g
● Sahaminta maraqyada hal-abuurka ah): 0.1-0.5g
● Hawada (xayawaan waaweyn): 0.5-2G
● Factos (jiirka): 3-5grus
Food Placenolar Minveage Funcsage: Waraaqda shaandhaynta
● Suuxdinta xubinta taranka: 5-6 swabs
● Maqaarka / warqadaha xubinta taranka / xubinta taranka
● microorganisms dusha sare: warqad shaandhee
● Biyo / hawada / biofalm: warqad shaandhee
● Edopytes: 1-2G
● Qalabka ilkaha: 0.5-1g

Socodka shaqada adeegga

Keenista muunad

Dhismaha Maktabadda

Kala xidowdo

Falanqaynta Xogta

Adeegyada ka dib

Hore: Bakteeriyada iyo fungal dhammaan dib-u-habeynta Genome

Xiga: 16s / 18s / eveickon sequoing-pacbio

Waxaa ku jira falanqaynta soo socota:

Xakamaynta tayada tayada ee ceeriin
Otu clustering / de-buuqa (asv)
Qoraalka Otu
Falanqaynta Kala-duwanaanta Alha: tusmooyin badan, oo ay ku jiraan Shannon, Simpson iyo Ace.
Falanqaynta Kala-Bixinta Beta
Falanqaynta Kooxda Kooxaha
Falanqaynta xiriirka: ee udhaxeeya arrimaha deegaanka iyo ka kooban iyo kala duwanaanshaha
16s saadaalin wadaha oo shaqeynaya

Histogram ee qaybinta cashuurta

cashuureed kululaynta badan ee kululaynta

Falanqaynta Kala-soo-dejinta Alfa: Qalooca Farafipe

Falanqaynta Kala-Bixinta Beta: NMDs

Falanqaynta Koox-kooxeed: Lefse Boomarker Discovery

Sahaminta Horumarrada ay fududeeyaan adeegyada isku xidhka filimka 'Brkgene' ee BMKGene 'oo ay ka qaataan Ilumina iyada oo loo marayo aruurinta daaqsinta daabacaadaha.

Dong, c. et al. (2022) 'Goluhu, Core Microbioga, oo ka shaqeeya ciidda roobka ah iyo jilifyada ukumoloiolos-ka ee ku yaal Microbiology', xuduudaha Microbiology, 5.3389 / FMICB.2022.8553117 / Buuxda.

Li, Y. et al. (2023) 'Connctetic-ka bakteeriyada isku-darka ah ee lagu tallaalayo daaweynta Garderlla Siilka Siilka Gardnellas-Wacnosis-ka oo ku dhaca jiirka', microbiome, 11 (1), pp. 1-14. doi: 10.1186 / s40168-023-01497-y

Yang, J., Fu, Y. iyo Liu, H. (H. (H. (2022) 'Microbiomes of boodhkii boodhku u soo ururiyey inta lagu gudajiray salka ku-meel-gaarka ah "Dawladda' '' '' '' '' '' '' ',, 1 (PP. 1-20. doi: 10.11186 / s40793-022-00399-0 / Tirooyin / 8.

Yin, S. Et al. (2022) 'Fluenstock-Feutstock-Ku tiirsanaanta hidda-wadayaasha shaqeeya ee la xiriira isbadalka nitrogen ee ay ka mid tahay luminta nitrogen-ka ee isku-darka', tikniyoolajiyadda Biorsource, 361, p. 127678. Do: 10.1016 / J.Biteech.2022.127678.