Yese Transprote Sequencing - Illumina
Zvimiro
● Tualibhurari kurayiridzo kutevedzana mharidzo yakazara.
● Zadzisa Bioinforcs kuongororwa kweMrna, LNCRRA, Circrna, naMirna muzvikamu zvakaparadzana zveBioinforms
● Kuongorora kwakabatana kwezvose rna kutaura mumushumo wakasanganiswa, kusanganisira cerna network kuongorora.
Zvigadzirwa zvebasa
●Mukudzika kuongororwa kwekudzokorora metulators network.
●Yakanyatsotaurwa Nyowani: Isu tinoshandisa madhiri akawanda kuti asave neyakawedzera kutaurwa geny
●Hunyanzvi hwakawanda.
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, tinopa chirongwa chekutevera, tenderera mberi nekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Kuongorora zano | Data yakakurudzirwa | Mhando yekudzora |
| rrna yakanyungudutswa | Illumina Pe150 | 16 GB | Q3085% |
| Saizi yakasarudzwa | Illumina se50 | 10-20m inoverenga |
Zvinodiwa Muenzaniso:
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 80 | ≥ 1.6 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | Rină6.0 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatics
Rna Express Overview
Zvakasiyana-siyana zvinoratidzwa majini
Cerna Analeysis
Zvakasiyana-siyana zvinoratidzwa mirnas uye zvinoenderana neRNAS
Ongorora mafambiro ekutsvaga ekuratidzira akafambisa neBMKGE 'Yese Transprome inoteedzera masevhisi kuburikidza nekuunganidzwa kwakazara kwemabhuku.
Dai, Y. et al. . " Doi: 10.1039 / d1mo00370d.
Liu, N. nan et al. . Doi: 10.1016 / J.Geno.2022.146503.
Wang, xj et al. . 904865. Doi: 10.3389 / Fanc.2022.904865 / Bibtex.
Xu, P. et al. . " Doi: 10.1186 / S12864-02-0842-022-08470-3 / Nights / 7.
Yan, z. et al. . 111878. DOI: 10.1016 / J.Postharvbio.2022.11187.
