Whole genome bisulfite sequencing (WGBS)
Whole Genome Bisulfite Sequencing (WGBS) inomira senzira yegoridhe-yakajairwa yekuongorora kwakadzama kweDNA methylation, kunyanya chinzvimbo chechishanu mu cytosine (5-mC), yakakosha inodzora yejini kutaura uye cellular chiitiko. Nheyo iri pasi peWGBS inosanganisira kurapwa kwebisulfite, kukonzeresa shanduko yeunmethylated cytosines kuita uracil (C kusvika kuU), ichisiya methylated cytosines isina kuchinjika. Iyi nzira inopa imwechete-base resolution, ichibvumira vaongorori kuti vaongorore zvizere iyo methylome uye kufumura abnormal methylation mapatani ane chekuita neakasiyana mamiriro, kunyanya cancer. Nekushandisa WGBS, masayendisiti anogona kuwana nzwisiso isingaenzaniswi mune genome-wide methylation landscapes, ichipa kunzwisisa kwakadzama kwemaitiro epigenetic ayo ari pasi pemaitiro akasiyana ebhayoloji nezvirwere.
Zvimiro Zvebasa
● Inoda referensi genome.
● Lambda DNA inowedzerwa kutarisa bisulfite kutendeuka kushanda zvakanaka.
● Sequencing paIllumina NovaSeq.
Basa Rinobatsira
●Goridhe Standard yeDNA Methylation Research: Iyi yakakura methylation shanduko yekugadzirisa tekinoroji ine yakanyanya kurongeka uye yakanaka kubereka.
●Wide Coverage uye Single-base Resolution:kuwanikwa kwenzvimbo dzemethylation pa genome-wide level.
●Complete Platform:ipa imwe-yekumira yakanakisa sevhisi kubva pakugadziriswa kwemuenzaniso, kuvaka raibhurari, kutevedza kune bioinformatics kuongororwa.
●Hunyanzvi Hwakawanda: nemapurojekiti ekutevedzanisa eWGBS akapedzwa zvakabudirira mumhando dzakasiyana-siyana dzezvipenyu, BMKGENE inounza anopfuura makore gumi echiitiko, timu yekuongorora ine hunyanzvi, yakazara zvemukati, uye yakanakisa post-sales rutsigiro.
●Kugona Kujoinha neTranscriptomics Analysis: kubvumira kuongororwa kwakabatanidzwa kweWGBS nedzimwe omics data seRNA-seq.
Sample Specifications
| Library | Sequencing Strategy | Inokurudzirwa kuburitsa data | Kudzora kwehutano |
| Bisulfite kurapwa | Illumina PE150 | 30x kudzika | Q30 ≥ 85% Bisulfite shanduko > 99% |
Muenzaniso Zvinodiwa
| Concentration (ng/µL) | Huwandu hwese (µg) | Zvimwe zvinodiwa | |
| Genomic DNA | ≥ 5 | ≥ 400 ng | Kuderedza kuderera kana kusvibiswa |
Kuyerera Kwebasa Rebasa
Muenzaniso wekutumira
Kutorwa kweDNA
Kuvakwa kweraibhurari
Sequencing
Data analysis
Data delivery
Inosanganisira ongororo inotevera:
● Raw sequencing quality control;
● Mepu yekutarisa genome;
● Kuonekwa kwe5mC methylated bases;
● Kuongororwa kwekugoverwa kwemethylation uye annotation;
● Kuongororwa kweMatunhu akasiyana-siyana eMethylated (DMRs);
● Tsanangudzo yekushanda yemajini ane chekuita neDMRs.
5mC methylation yekuona: marudzi emasaiti ane methylated
Methylation mepu. 5mC methylation genome-wide distribution
Tsanangudzo yenzvimbo dzakanyanya methylated
Differentially Methylated Regions: akabatana majini
Differentially Methylated Regions: rondedzero yemajini akabatana (Gene Ontology)
Ongorora kufambira mberi kwekutsvagisa kunofambiswa neBMKGene's yese genome bisulfite sequencing masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
Fan, Y. et al. (2020) 'Ongororo yeDNA methylation profiles panguva yegwai skeletal tsandanyama kukura uchishandisa yakazara-genome bisulfite sequencing',BMC Genomics, 21(1), map. 1–15. doi: 10.1186/S12864-020-6751-5.
Zhao, X. nevamwe. (2022) 'Novel deoxyribonucleic acid methylation kukanganisa muvashandi vakafumurwa kune vinyl chloride',Toxicology uye Industrial Health, 38(7), mapeji 377–388. doi: 10.1177/07482337221098600
Zuo, J. nevamwe. (2020) 'Hukama huri pakati pegenome methylation, mazinga easina-coding RNAs, mRNAs uye metabolites mukuibva muchero wematomatisi',The Plant Journal, 103(3), mapeji 980–994. doi: 10.1111/TPJ.14778.
