TGuide S16 Nucleic Acid Extractor
Product sumo
TGuide S16 Nucleic Acid Extractor inogona kugadzira anosvika gumi nematanhatu sampuli panguva imwe chete nemagineti tsvimbo kusunga uye kuendesa magineti mabhero, iyo 96-tsime ndiro kana imwe-sample reagent cartridge uye magineti tip comb. Nekushandisa inoenderana nucleic acid yekubvisa reagent, chigadzirwa ichi chinogona kuchenesa DNA/RNA kubva kwakasiyana sampuli dzemhuka, chirimwa, mabhakitiriya, fungi, nezvimwewo, uye kubvisa DNA kubva muplasmid, agarose gel uye PCR chigadzirwa.
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Features
Super nyore kushandisa
Hapana basa rekuwedzera pombi. Iyo elution vhoriyamu inogona kugadziridzwa sezvinodiwa.
Kuiswa uye kushanda zvinoda kudzidziswa kushoma. Nezvirongwa zvakagara zvakaiswa, bvisa katuriji, sarudza protocol uye mhanyisa kuyedza kwako. Zvirongwa zvakagadzirira-kushandisa, uye zvakare customizable.
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New binding mode
Iyo magineti tsvimbo inotora yakasimba yekupedzisira inosunga maitiro.
Iwo magineti mabedhi anosunga kuzasi kwetsvimbo, izvo zvinove nechokwadi kuti iyo enzuru inogona kuramba yakavhara ese magineti mabedhi kunyangwe vhoriyamu iri diki. Iyo dhizaini inowedzera goho reiyo nucleic acid.
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Yakakwana dhizaini yekudziya tangi
Pane mbiru yakaiswa pakati pe lysis buffer uye eluent column, iyo ino dzivirira zvinobudirira kubva pakubuda nekuda kwe lysis column kudziyisa. Naizvozvo, kudzoreredza vhoriyamu yeiyo nucleic acid mhinduro yakanyatsojeka.
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Contamination control uye kuchengetedzwa kwemushandisi
Inogona kuiswa pabhenji yakachena kana mumakemikari hood.
Chiridzwa chiri kuita otomatiki uye chakavharwa.
Iyo yekusvibisa yekudzora sisitimu inotora nzira mbiri: yemukati-yemukati-kusvibiswa control system uye ultraviolet sterilization system, kudzivirira zvakanyanya kusvibiswa pakati pemakoramu nemasampuli.
Preloaded reagents uye anofananidzwa anoraswa consumables.
Iyo kemikari kubata inobviswa kusvika pamwero mukuru kuti uve nechokwadi chekuchengetedzwa kwekushanda.
Demo Results
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Muedzo mhedzisiro: kushandisa TGuide S16 ine reagent TGuide Smart Universal DNA Kit (4993704)
kuburitsa otomatiki genomic DNA yemasampuli akawanda anosanganisira maseru, tishu, ropa, gwapa reropa rakaomeswa (DBS), Lactobacillus, E.coli, mate, uye swab, zvichingodaro, kuwana goho rakanaka uye kuchena kwakanyanya.
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Iyo OD260/OD280 yakatenderedza 1.7 ~ 1.9, uye
OD260/OD230＞1.6.
Elution vhoriyamu: 100 μl
Agarose gel concentration: 1.5%
Kurodha vhoriyamu: 1 μl
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Kushandisa TGuide S16 ine reagent TGuide Smart Viral DNA/RNA Kit (4993702)
kuburitsa otomatiki DNA / RNA kubva kune akawanda masosi anosanganisira ropa rose, swab uye plasma yakawana goho rakanaka uye kuchena kwakanyanya. -
Iyo OD260/OD280 yakatenderedza 1.7~1.9, uye OD260/OD230 ~ 1.6.
Kambani & B kambani: Anozivikanwa mhando.
ND: Chirwere cheNewcastle, H5:Avian flu, CP: Canine parvovirus, ASF: African Swine fever virus
Iyo shoma yekunzwa inogona kusvika 100 makopi / ml.
Mhedzisiro yemhedzisiro: kushandisa TGuide S16 ine reagent TGuide Smart Ropa Genomic DNA Kit (4993703) kuburitsa otomatiki genomic DNA yeropa rakaomeswa yawana goho rakanaka uye kuchena kwepamusoro, izvo zvakaenzana negoho rekubvisa uye kuchena kwezvinhu zvinozivikanwa zvinokwikwidza.
Iyo OD260/OD280 yakatenderedza 1.7~1.9, uye OD260/OD230 ~ 1.7.
Sample source: Matatu eropa revanhu rose. Matatu akadzokorora miedzo yemakiti ega ega.
Replicates yekiti yega yega inobva kune imwechete sample sosi.
Mamiriro emuenzaniso: Ropa rakaomeswa
Kupinza vhoriyamu: 200 μl
Elution vhoriyamu: 100 μl
Agarose gel concentration: 1.5%
Kurodha vhoriyamu: 1 μl
M: Marker III, TIANGEN
Kambani & B kambani: Anozivikanwa mhando
2000 bp chikamu agarose gel DNA kucheneswa uye kupora
Agarose gel concentration :1.5% (TBE)
Kurodha vhoriyamu: 8 μl
Mucherechedzo: D2000, TIANGEN
S16-01, S16-02, uye S16-03 inomiririra kuenzanisa sampuli yekuchenesa uye kuedza kupora uchishandisa 3 TGuide S16 Nucleic Acid Extractors neTGuide Smart DNA Purification Kit (4993550) panguva imwe chete.
Kambani: iyo spin-column yekubvisa kit yemhando inozivikanwa
Agilent 2100 yakashandiswa pakuona kutendeseka kweRNA.
Sample source: CD-1 mice
Chimiro chemuenzaniso: ropa idzva rose
Saizi yemuenzaniso: 200 μl
Muedzo wemhedzisiro: iyo yakazara RNA yakatorwa kubva muropa neTGuide S16 yakashongedzerwa neTGuide Smart Ropa / Cell / Tissue RNA Kit (4993551) ine goho rakanaka uye kuchena kwepamusoro, uye inogona kusangana nezvinodiwa zvekudzika kwepamusoro-kuburikidza kutevedzana.
Kurodha vhoriyamu: 1 μl. Chiropa chakaderedzwa ka5 uye chakatakurwa. Agarose gel concentration: 1%. Electrophoresis ye 20 min pa 6 V / cm;
M: TIANGEN Marker III
1-4: mwoyo 5-8: chiropa 9-12: mapapu 13-16: itsvo
Masampuli maviri ekutanga etishu imwe neimwe akatorwa ne-spin-column based extraction kit, uye maviri ekupedzisira akabudiswa otomatiki neTGuide S16.
Sample source: CD-1 mice
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Muedzo mhedzisiro: mhuka yemhuka genomic DNA inotorwa otomatiki neTGuide S16 ine reagent TGuide Smart Magnetic Tissue DNA Kit (4993547) ine goho rakakura uye kuchena, iyo yakaenzana neDNA yakatorwa ne spin column-based extraction protocol. Kuchena kwakangofanana, neOD260/OD280 yakatenderedza 1.7~1.9 uye OD260/OD230> 1.7. Mukupedzisa, TGuide S16 inogona kushandiswa kubvisa iyo genomic DNA kubva munyama yemhuka mukutsiva iyo spin-column based extraction solution.
Yese RNA kudhirowa kubva kwakasiyana zvirimwa samples
Saizi yemuenzaniso: 100 mg
Sample pre-kurapwa: yakaderera tembiricha homogenizer
Agarose gel concentration: 1% (TAE)
Kurodha vhoriyamu: 1 μl
M: Mucherechedzo III, TIANGEN
1-4: mhodzi yechibage 5-8: mashizha echibage 9-12: midzi yekabichi 13-16: soya beans
Mienzaniso miviri yekutanga yakatorwa ne-spin-column extraction kit uye masampuli maviri ekupedzisira akatorwa neTGuide S16.
Genomic DNA kubviswa kwegorosi midzi
Saizi yemuenzaniso: 100 mg
Sample pre-treatment: kukuya ne nitrogen yemvura kana kuti tissue grinding homogenizer
Agarose gel concentration: 1% (TAE)
Kurodha vhoriyamu: 2 μl
Mucherechedzo: D15000, TIANGEN
Kambani: zita rinozivikanwa
Muedzo mhedzisiro: kushandisa TGuide S16 ine reagent TGuide Smart Magnetic Plant DNA Kit (4993548) kuti ibvise otomatiki iyo genomic DNA yegorosi midzi yakawana goho rakanaka uye kuchena kwepamusoro, izvo zvakaenzana negoho rekuchera uye kuchena kweanozivikanwa anokwikwidza. Uyezve, kuvimbika kwegenomic DNA kuri nani pane izvo zvinokwikwidza zvigadzirwa zvemamwe makambani. Mukuedza uku, inenge 15 μg ye nucleic acid yakatorwa kubva ku100 mg midzi yegorosi, ine OD260 / OD280 yakapoteredza 1.8 ~ 1.9, uye OD260 / OD230＞2.0.
