Diki rna sequencing-Illumina
Zvimiro
● Kugadzirira kugadzirira kunosanganisira saizi yekusarudza nhanho
● Bioinformatic ongororo yakatenderedza Michena kufanotaura nezvinangwa zvavo
Zvigadzirwa zvebasa
●Yakakwana bioinformatics kuongororwa:Kugonesa kuzivikanwa kwezviziviso zvese uye novel mirnas, kuzivikanwa kweMirnas zvinangwa, uye zvinoenderana nekuita basa rekushanda uye nekuvandudza neyakawandisa madhiri (kegg, enda)
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
●Hunyanzvi hwakawanda.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Chikuva | Yakakurudzirwa data | Data qc |
| Saizi yakasarudzwa | Illumina se50 | 10m-20m kuverenga | Q3085% |
Zvinodiwa Muenzaniso:
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | RINO6.0; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
● Zvirimwa:
Midzi, tsinde kana petal: 450 mg
Shizha kana Mbeu: 300 mg
Michero: 1.2 g
● Mhuka:
Moyo kana mudumbu: 450 mg
Viscera kana uropi: 240 mg
Muswe: 600 mg
Mapfupa, bvudzi kana ganda: 1.5g
● Arthropood:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rese: 2 machubhu
● Masero: 106 masero
● Serum uye plasma:6 ml
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatics
● Raw Data Rekutonga
● Srna kuiswa
● Kuunganidzwa kune chirevo genome
● Kuzivikanwa kwechizivikanwa uye novel Mirna
● Kusiyanisa Mirna Express kuongorora
● Inoshanda Annotation of Mirna Target
Kuzivikanwa kweMirna: dhizaini uye kudzika
Kutaura kwakasiyana kweMirna - Hiearchcal clustering
Inoshanda Information yeTarget yetsika yakaratidza Mirnas
Ongorora kufambira mberi kwekutsvaga kwakabatana neBMKGE 'SRN Sequente Services kuburikidza nekuunganidzwa kwakasarudzika kwemabhuku.
Chen, H. Et Al. . 108038. DII: 10.1016 / J.Pplaphy.2023.108038.
Li, H. Et Al. . Doi: 10.15252 / embri2255037 / Supl_file / EMB202255037-SH7-SH-0004-SDatafig4.FIF.
Yu, J. et al. (2023) 'Microrna AMME-BANTM-3P inodzora Bulul Development nekumusoro-smagen 8 . 5726. Dii: 10.390 / ijms24065726 / s1.
Zhang, M. Et Al. . Doi: 10.1159 / 000489649.
