Yakatemwa inomiririra bisulfite sequencing (rrbs)
Yakadzikira Nepo wgbs inopa ruzivo rwakakwana nekuongorora iyo yese genome pakugadzirisa kwesarudzo, mutengo wakakwira unogona kuve chinhu chinogumira. RRBs zvine musoro kudzikisira dambudziko iri nekusarudza kuongorora chikamu chechikamu cheiyo genome. Iyi nzira inovimba nekuvimbika kweCPG matunhu echitsuwa-akapfuma neSSPI Cleavage inoteverwa nekutsanangurwa kwekusarudzwa kwe200500 / mazana matanhatu ezvimisikidzwa zvidimbu. Nekuda kweizvozvo, chete matunhu proximal kune zvitsuwa zveCPG anoteererwa, nepo avo vane nzvimbo dziri kure dzeCPG isisisirwe kubva mukuongororwa. Kuita uku, kwakabatanidzwa ne bisulfite kutsakatika kweDNA methyclation yeDNA, uye nzira yekutsungirira, Pe150, inotarisa zvakananga pamigumo yemapepa pane nepakati, inowedzera kunaka kwemakurukota. Iyo rrbs chishandiso chakakosha icho chinogonesa mutengo-unoshanda DNAYLARLATION RWORK uye kufambira mberi ruzivo rweEpigenetic mechanisms.
Service maficha
● Inoda chirevo chemberi.
● Lambda DNA inoshandiswa kuongorora kushandurwa kweBisulfite kushanda.
● MSPI Deraestion inoshanda zvakare inotariswa.
● Kaviri enzyme kugaya kwemasangano ezvidyarwa.
● Kuongorora kuIllumina novaseq.
Zvigadzirwa zvebasa
●Mutengo-unobudirira uye unoshanda neimwe nzira kune wgbs: Kugonesa kuongororwa kunoitwa pamutengo wakaderera uye uine zvakadzika zviyero zvinodiwa.
●Zadzisa chikuva:Ipa imwe-simisa zvakanaka basa kubva mukuenzanisira, kuvaka kuvaka, uye kutevedzana kuenda kuBioinfortics kuongororwa.
●Hunyanzvi hwakawanda.
Service Rudzidziso
| Raibhurari | Kuongorora zano | Yakakurudzirwa Kubuda kweData | Mhando yekudzora |
| MSPi akadaira uye bisulfite raibhurari | Illumina Pe150 | 8 GB | Q30 × 85% Bisulfite Shanduko> 99% MSPI Kucheka Kubudirira> 95% |
Zvinodiwa Muenzaniso
| Concentration (ng / μL) | Mari Yese (μG) |
| |
| Genomic DNA | ≥ 30 | ≥ 1 | Kusvibiswa kusvibiswa kana kusvibiswa |
Basa rebasa rinoyerera
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Inosanganisira kuongororwa kunotevera:
● Raw kutevedza kunaka kwemhando;
● Mepu kurevesa genice;
● Kuwanikwa kwe5mc methylated zvigadziko uye motif chiratidzo;
● Kuongororwa kwekugovera methylation kugovera uye kuenzanisa kwemuenzaniso;
● Kuongororwa kwezvinhu zvakasiyana methylated (DMRS);
● Kuongorora kwezviratidzo zvejini kwakabatana neDMRS.
Mhando Yekudzora: Digestion Kubudirira (muGenome Mepu)
Mhando Yekudzora: Bisulfite Shanduro (muMethycation Info Exction)
Mepu Mepu: 5mc methylation genome-yakakura kugoverwa
Sampuli Enzanisa: Prippal Commence ongororo
Zvakasiyana methylated matunhu (DMRS) kuongororwa: Heatmap
Ongorora kufambira mberi kwekutsvaga kwakabatanidzwa neBMKGERI Yese Bislffite Inequencem Sequencing Services kuburikidza nekuunganidzwa kwakasarudzika kwemabhuku.
Li, z. et al. .Pnas Nexus, 1 (4). Doi: 10.1093 / pnasnexus / PGAC179.
Tian, H. Et Al. . "European Jurnal yeClinical kuongorora, 53 (11), p. E14055. Doi: 10.1111 / ECI.14055.
Wu, Y. et al. .Chinyorwa cheEndocrinological kuongorora, 45 (12), pp. 2365-276. Doi: 10.1007 / S40618-022-01878-4.
