Prokaryotic rna sequencing
Zvimiro
● Rna maitiro ekugadzirisa aisanganisira RRN Flight inoteedzerwa neyambiro yeRna Library kugadzirira.
● Bioinformatic Ongororo Yakavakirwa Pazvinongement kune Reference
.
Zvigadzirwa zvebasa
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.
●Strand-chaiyo kutevedza data.
●Kuongororwa kwakazara kwakarongedzwa kuProkaryotic Transpilation. Izvo zvinosanganisirawo kuongororwa kwernas, zita rinoti uye kufanotaura kwechipiri chimiro uye zvinangwa.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Kuongorora zano | Data yakakurudzirwa | Mhando yekudzora |
| rrna yakadzikira raibhurari raibhurari | Illumina Pe150 | 1-2 GB | Q3085% |
Zvinodiwa Muenzaniso:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 50 | ≥ 1 | Od260 / 280 = 1.8-2.0 Od260 / 230 = 1.0-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | Rin≥6.5 |
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatic Ongororo Yekushandisa
Inosanganisira kuongororwa kunotevera:
● Raw Data Rekutonga
● Kuunganidzwa kune chirevo genome
● Raibhurari Unhu hwekuongorora: rna kufungidzira rombo, kuisa saizi uye kutsakatisa saturation
● Inoshanda Information yeakafungidzirwa kuunganidza majenji
● Kuongorora kwezvirevo: Correlation uye Prompl Component Ongorora (PCA)
● Kutaura kwakasiyana kweGene (DEGS)
● Inoshanda Ingationation uye Kufumisa kweDGS
● Srna kuongorora: kufanotaura, chirevo, chinangwa, uye chechipiri chimiro chekufungidzira
● Transcript Yakasarudzika Ongororo: Operimi, kutanga uye kuguma nzvimbo, dunhu risiri rechokwadi (UTS), Purmoter, uye SnP / Indil Ongorora
Kutevedzana kwekutsvagisa
Inoshanda Information yeCoding majini
Kuwirirana pakati samples
Kusiyanisa kwakaratidzwa majini (degs) kuongororwa
Inoshanda Kuongorora Kwekuongorora
Srna Annotation
Ongorora kufambira mberi kwakabatanidzwa neBMKGE aN's NAPOPORE yakazara-urefu MrN
Guan, cp et al. . "Poland Chinyorwa cheMicrobiology, 67 (2), p. 223. DOI: 10.21307 / PJM-2018-024.
