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BMKMANU S3000_Spatial Transcriptome
Spatial transcriptomics inomira kumberi kwehunyanzvi hwesainzi, ichipa simba vaongorori kuti vatarise mumatanho ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epakati. Pakati pemapuratifomu akasiyana, BMKGene yakagadzira iyo BMKManu S3000 Spatial Transcriptome Chip, ichizvikudza yakagadziridzwa kugadzirisa kwe3. Iyo S3000 chip, ine nzvimbo dzinosvika mamirioni mana, inoshandisa madiki madiki akaturikidzwa nemabead akaiswa ane spatially barcoded capture probes. Iyo cDNA raibhurari, yakafumiswa ne spatial barcode, inogadzirwa kubva kuS3000 chip uye inozoteedzerwa paIllumina NovaSeq papuratifomu. Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa. Iyo BMKManu S3000 chip's inoshanda zvakasiyana-siyana, ichipa akawanda-level resolution marongero ayo anogona kunyatsokwenenzverwa kune akasiyana matishu uye anodiwa mazinga eruzivo. Uku kuchinjika kunoisa chip sechisarudzo chakatanhamara chezvakasiyana zve spatial transcriptomics zvidzidzo, kuve nechokwadi chechokwadi chekubatana kwenzvimbo ine ruzha rushoma. Iko kushandiswa kwesero segmentation tekinoroji neBMKManu S3000 inogonesa kuganhurwa kwedhata rekunyora kumiganhu yemasero, zvichikonzera ongororo ine yakananga biological zvinoreva. Uyezve, kugadziriswa kwakagadziridzwa kweS3000 kunoguma nehuwandu hwemajini uye UMIs inoonekwa pasero, zvichiita kuti kuongororwa kwakanyatsojeka kwemaitiro ekunyora kwepakati uye kuunganidza kwemasero.
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Single-nuclei RNA Sequencing
Kuvandudzwa kwekutorwa kwesero rimwe chete uye tsika dzekuvaka raibhurari, pamwe chete nepamusoro-soro kutevedzana, yakashandura zvidzidzo zvejini padanho resero. Kubudirira uku kunobvumira kuongororwa kwakadzama uye kwakadzama kwehuwandu hwemasero akaomarara, kukunda zvipingamupinyi zvine chekuita neavhareji yekutaura kwemajini pamusoro pemasero ese uye kuchengetedza heterogeneity yechokwadi mukati mehuwandu hwevanhu. Nepo single-cell RNA sequencing (scRNA-seq) ine mabhenefiti asingarambike, inosangana nematambudziko mune mamwe matissue uko kugadzirwa kwesero-sero kumiswa kunoratidza kwakaoma uye kunoda sampuli nyowani. PaBMKGene, tinogadzirisa chipingamupinyi ichi nekupa imwechete-nucleus RNA sequencing (snRNA-seq) tichishandisa iyo-ye-iyo-ye-iyo 10X Genomics Chromium tekinoroji. Iyi nzira inowedzera kuwanda kwemasamples anogoneka kuongororo yezvinyorwa padanho resero rimwe chete.
Kuparadzaniswa kweiyo nuclei kunoitwa kuburikidza neyakagadziridzwa 10X Genomics Chromium chip, ine sere-chiteshi microfluidics system ine kaviri kuyambuka. Mukati megadziriro iyi, mabheji ejeri anosanganisira mabhakodhi, maprimers, enzymes, uye nucleus imwe chete yakavharidzirwa mumadonhwe emafuta e-nanoliter, achigadzira Gel Bead-in-Emulsion (GEM). Kutevera kuumbwa kweGEM, cell lysis uye barcode kuburitswa kunoitika mukati meGEM yega yega. Zvadaro, mRNA mamorekuru anonyorerwa reverse mucDNAs, inobatanidza 10X barcode uye Unique Molecular Identifiers (UMIs). Aya ma cDNAs anobva aiswa pasi peyakajairwa kuteedzera raibhurari kuvaka, kufambisa kwakasimba uye kwakadzama kuongororwa kwemajini ekutaura profiles padanho resero rimwe.
Platform: 10 × Genomics Chromium uye Illumina NovaSeq Platform
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics tekinoroji yekucheka-kumucheto iyo inobvumira vaongorori kuti vaongorore maitiro ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epamhepo. Imwe puratifomu ine simba mune ino domain ndeye 10x Genomics Visium yakasanganiswa neIllumina kutevedzana. Iyo musimboti we10X Visium iri pane yakasarudzika chip ine yakasarudzika nzvimbo yekubata inoiswa zvikamu zvetishu. Iyi nzvimbo yekubata ine barcoded mavara, imwe neimwe inoenderana nenzvimbo yakasarudzika mukati mematishu. Iwo akabatwa maRNA mamorekuru kubva munyama anobva anyorwa neakasarudzika mamorekuru identifiers (UMIs) panguva yekudzokesa kunyorwa maitiro. Aya mabarcoded spots uye UMIs anogonesa chaiyo spatial mepu uye quantification yegeneti kutaura pane imwechete-sero resolution. Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa. Nekushandisa iyi Spatial Transcriptomics tekinoroji, vaongorori vanogona kuwana nzwisiso yakadzama yekurongeka kwenzvimbo yemasero uye kupindirana kwakaoma kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumatanho ari pasi pemaitiro ezvipenyu mundima dzakawanda, kusanganisira oncology, neuroscience, developmental biology, immunology. , uye zvidzidzo zvebotanical.
Platform: 10X Genomics Visium uye Illumina NovaSeq
