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DNBSEQ pre-yakagadzirwa maraibhurari
DNBSEQ, yakagadziridzwa neMGI, inyowani yeNGS tekinoroji yakakwanisa kudzikisira kudzika kwemitengo yekutevedzana uye kuwedzera kubuda. Kugadzirira kwemaraibhurari eDNBSEQ kunosanganisira kupatsanurwa kweDNA, kugadzirira ssDNA, uye kutenderedza denderedzwa amplification kuti uwane iyo DNA nanoballs (DNB). Izvi zvinobva zvatakurwa panzvimbo yakasimba uye zvichizoteedzerwa necombinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ tekinoroji inosanganisa mabhenefiti ekuva neakaderera amplification kukanganisa mwero nekushandisa yakakwira density kukanganisa mapatani nemananoballs, zvichikonzera kutevedzana nepamusoro maburi uye nemazvo.
Yedu yakafanogadzirwa raibhurari sequencing sevhisi inoita kuti vatengi vagadzirire Illumina kuteedzera maraibhurari kubva kwakasiyana masosi (mRNA, yakazara genome, amplicon, 10x maraibhurari, pakati pevamwe), ayo anochinjirwa kumaraibhurari eMGI mumarabhoritari edu kuti ateedzerwe muDNBSEQ-T7, ichigonesa. data yakakwira pamitengo yakaderera.
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Illumina pre-yakagadzirwa maraibhurari
Illumina sequencing tekinoroji, yakavakirwa paSequencing by Synthesis (SBS), innovation yakambundirwa pasi rose yeNGS, ine basa rekugadzira inopfuura makumi mapfumbamwe muzana yedata renyika. Nheyo yeSBS inosanganisira kufungidzira fluorescently yakanyorwa reversible terminators sezvo dNTP yega yega inowedzerwa, uye yakazonamirwa kubvumira kubatanidzwa kweiyo chigadziko chinotevera. Nezvose zvina zvinodzoreredzwa zveterminator-yakasungwa dNTPs dziripo mune yega yega kutevedzana kutenderera, makwikwi echisikigo anoderedza kubatanidza kusarerekera. Iyi tekinoroji inosiyana-siyana inotsigira ese ari maviri-averengeka uye maviri-ekupedzisira-maraibhurari, achiitira kune akasiyana genomic application. Illumina sequencing's high-throughput kugona uye nemazvo inoisa iyo senheyo yekona mukutsvagisa genomics, ichipa simba masayendisiti kuburitsa hunyoro hwema genomes ane ruzivo rusingaenzaniswi uye kugona.
Yedu yakafanogadzirwa raibhurari sequencing sevhisi inogonesa vatengi kugadzirira kuteedzera maraibhurari kubva kwakasiyana masosi (mRNA, yakazara genome, amplicon, 10x maraibhurari, pakati pezvimwe). Zvadaro, maraibhurari aya anogona kutumirwa kunzvimbo dzedu dzekutevedzana kwehutongi hwemhando uye kutevedzana mumapuratifomu eIllumina.
