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Kuenzanisa Genomics
Kuenzanisa genomics kunosanganisira kuongororwa uye kuenzanisa kwese genome kutevedzana uye zvimiro pakati pemarudzi akasiyana. Iyi ndima inotsvaga kufumura kushanduka kwezvipenyu, decode mashandiro emajini, uye kujekesa maitiro ekutonga genetic nekuona zvakachengetwa kana kupatsanurwa kutevedzana zvimiro uye zvinhu muzvisikwa zvakasiyana siyana. Chidzidzo chakakwana chekuenzanisa genomics chinosanganisira ongororo dzakadai semhuri dzemajini, kusimukira kwemhindumupindu, zviitiko zvekudzokorora-zvese-genome, uye kukanganisa kwekumanikidza kwakasarudzika.
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Evolutionary Genetics
Huwandu hwevanhu uye evolutionary genetic analysis platform inovambwa pahwaro hwechiitiko chikuru chakaunganidzwa mukati meBMK R&D timu kwemakore. Icho chishandiso chine hushamwari mushandisi kunyanya kune vaongorori vasiri hukuru mune bioinformatics. Iyi puratifomu inogonesa evolutionary genetics ine chekuita nekuongorora kwekutanga kunosanganisira phylogenetic muti kuvaka, yekubatanidza disequilibrium kuongororwa, genetic kusiyana kwekuongorora, yakasarudzika kutsvaira, kuongororwa kwehukama, PCA, kuongororwa kwehuwandu hwevanhu, nezvimwe.
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Hi-C Yakavakirwa Genome Assembly
Hi-C inzira yakagadzirirwa kubata chromosome kumisikidzwa nekubatanidza kuongorora kudhuze-kwakavakirwa kupindirana uye yakakwirira-kuburikidza sequencing. Kusimba kwekudyidzana uku kunofungidzirwa kuve kwakabatana zvisina kunaka nekureba kwemuviri pamakromosomes. Naizvozvo, data reHi-C rinoshandiswa kutungamira kuunganidzwa, kurongeka, uye kutungamira kweakaunganidzwa akateedzana mugwaro rejenome uye kusimudzira iwo pane imwe nhamba yemakromosomes. Iyi tekinoroji inogonesa chromosome-level genome musangano mukushaikwa kwehuwandu-hwakavakirwa genetic mepu. Yese genome imwe inoda Hi-C.
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Chirimwa/Mhuka De Novo Genome Sequencing
De Novokutevedzana (sequencing) zvinoreva kuvakwa kwejenome rerudzi rwemhando yose pachishandiswa matekinoroji ekutevedzana pasina genome rereferenzi. Nhanganyaya uye kutekeshera kwakapararira kwechizvarwa chechitatu kutevedzana, kuine kuverenga kwenguva refu, kwakasimudzira zvakanyanya kusangana kwejenome nekuwedzera kupindirana pakati pekuverenga. Kuwedzeredzwa uku kunonyanya kukosha kana uchibata neanonetsa genomes, akadai seaya anoratidza yakakwira heterozygosity, chiyero chepamusoro chenzvimbo dzinodzokororwa, mapolyploid, uye matunhu ane zvinhu zvinodzokororwa, zvisingaite zveGC zvirimo, kana kuomarara kwakanyanya kunowanzo kuunganidzwa zvisina kunaka uchishandisa pfupi-kuverenga kutevedzana. ndega.
Yedu-yekumira mhinduro inopa yakasanganiswa kutevedzana masevhisi uye bioinformatic ongororo iyo inopa yemhando yepamusoro de novo yakaunganidzwa genome. Ongororo yekutanga genome neIllumina inopa fungidziro yehukuru hwegenome uye kuomarara, uye ruzivo urwu runoshandiswa kutungamira nhanho inotevera yekuverenga kwenguva refu kutevedzana nePacBio HiFi, inoteverwa nede novomusangano we contigs. Iko kunotevera kushandiswa kweiyo HiC gungano kunogonesa kusungirirwa kweiyo contigs kune genome, kuwana chromosome-level musangano. Chekupedzisira, iyo genome inotsanangurwa nekufanotaura kwemajini uye nekuteedzera majini akatsanangurwa, achitendeukira kune zvinyorwa zvine pfupi uye refu kuverenga.
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Kutevedzana Kwemunhu Yese Exome
Human Whole exome sequencing (hWES) inobvumwa zvakanyanya senzira inodhura uye ine simba yekutevedzanisa yekunongedza machinjiro anokonzera chirwere. Kunyangwe ichingoumba chete 1.7% yegenome yese, maexons anoita basa rakakosha nekuratidza zvakananga chimiro chehuwandu hwemapuroteni. Sezvineiwo, mugenome remunhu, pamusoro pe85% yekuchinja kune hukama nezvirwere kunoratidzwa mukati meprotein coding matunhu. BMKGENE inopa yakazara uye inochinjika yemunhu yese exome sequencing sevhisi ine maviri akasiyana eexon ekutora nzira dziripo dzekuzadzisa zvinangwa zvakasiyana zvekutsvaga.
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
High-throughput genotyping, kunyanya pahuwandu hwevanhu, inhanho inokosha muzvidzidzo zvemajini ekubatana uye inopa genetic hwaro hwekuwanikwa kwemajini anoshanda, ongororo yekushanduka-shanduka, zvichingodaro. Panzvimbo pekutevedzana kwakadzika kwegenome,Yakaderedzwa Representation Genome Sequencing (RRGS)inowanzo shandiswa muzvidzidzo izvi kuderedza kuteedzera mutengo pasampu yega uchichengetedza kugona kwakanaka mukuwanikwa kwema genetic marker. RRGS inowana izvi nekugaya DNA ine kudzora ma enzymes uye kutarisa pane chaiyo chidimbu saizi renji, nekudaro ichitevedza chikamu chidimbu chejenome. Pakati peakasiyana maRRGS maitiro, Yakananga-Locus Amplified Fragment Sequencing (SLAF) inzira inogoneka uye yemhando yepamusoro. Iyi nzira, yakagadziridzwa yakazvimiririra neBMKGene, inokwidziridza iyo inorambidza enzyme yakaiswa kune yega purojekiti. Izvi zvinogonesa kugadzirwa kwehuwandu hwakakura hwema tag eSLAF (400-500 bps matunhu ejenome ari kutevedzana) ayo akagovaniswa zvakafanana mukati megenome uku achinyatso kudzivirira kudzokorora matunhu, nekudaro achivimbisa yakanakisa genetic marker kuwanikwa.
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Illumina pre-yakagadzirwa maraibhurari
Illumina sequencing tekinoroji, yakavakirwa paSequencing by Synthesis (SBS), innovation yakambundirwa pasi rose yeNGS, ine basa rekugadzira inopfuura makumi mapfumbamwe muzana yedata renyika. Nheyo yeSBS inosanganisira kufungidzira fluorescently yakanyorwa reversible terminators sezvo dNTP yega yega inowedzerwa, uye yakazonamirwa kubvumira kubatanidzwa kweiyo chigadziko chinotevera. Nezvose zvina zvinodzoreredzwa zveterminator-yakasungwa dNTPs dziripo mune yega yega kutevedzana kutenderera, makwikwi echisikigo anoderedza kubatanidza kusarerekera. Iyi tekinoroji inosiyana-siyana inotsigira ese ari maviri-averengeka uye maviri-ekupedzisira-maraibhurari, achiitira kune akasiyana genomic application. Illumina sequencing's high-throughput kugona uye nemazvo inoisa iyo senheyo yekona mukutsvagisa genomics, ichipa simba masayendisiti kuburitsa hunyoro hwema genomes ane ruzivo rusingaenzaniswi uye kugona.
Yedu yakafanogadzirwa raibhurari sequencing sevhisi inogonesa vatengi kugadzirira kuteedzera maraibhurari kubva kwakasiyana masosi (mRNA, yakazara genome, amplicon, 10x maraibhurari, pakati pezvimwe). Zvadaro, maraibhurari aya anogona kutumirwa kunzvimbo dzedu dzekutevedzana kwehutongi hwemhando uye kutevedzana mumapuratifomu eIllumina.
