-
Metagenomic Sequencing -NGS
Metagenome muunganidzwa weiyo yakazara genetic zvinhu yenharaunda yakasanganiswa yezvipenyu, senge zvakatipoteredza uye metagenomes yevanhu. Iine genomes ezvose zvinorimwa uye zvisingarimike tupukanana. Shotgun metagenomic sequencing neNGS inogonesa kudzidza kweaya akaomarara genomic landscapes akaiswa mumasample ezvakatipoteredza nekupa zvinopfuura taxonomic profiling, ichipawo ruzivo rwakakura mukusiyana kwemarudzi, kuwanda kwemasimba, uye kuoma kwehuwandu hwevanhu. Kupfuura zvidzidzo zvetaxonomic, pfuti metagenomics inopawo inoshanda genomics maonero, ichigonesa kuongororwa kwemajini akavharidzirwa uye mabasa awo ekuisa mumaitiro ezvisikwa. Chekupedzisira, kugadzwa kwematanho ekubatanidza pakati pema genetic zvinhu uye zvakatipoteredza zvinokonzeresa kunzwisiswa kwakazara kwekudyidzana kwakaomarara pakati penharaunda dzetwudiki nemamiriro avo ezvakatipoteredza. Mukupedzisa, metagenomic sequencing inomira sechimbo chakakosha kuburitsa kuomarara kwegenomic kwenharaunda dzakasiyana siyana dzehutachiona, kuvhenekera hukama hwakasiyana-siyana pakati pemajini uye ecology mukati meiyi yakaoma ecosystems.
Mapuratifomu: Illumina NovaSeq uye DNBSEQ-T7
-
Metagenomic Sequencing-TGS
Metagenome muunganidzwa wezvakagadzirwa zvemajini enharaunda yakasanganiswa yezvipenyu, senge zvakatipoteredza uye metagenomes dzevanhu. Iine genomes ezvose zvinorimwa uye zvisingarimike tupukanana. Metagenomic sequencing inogonesa kudzidza kweaya akaomarara genomic landscapes akaiswa mune ecological samples nekupa zvinopfuura taxonomic profiling. Iyo zvakare inopa inoshanda genomics maonero nekuongorora encoded majini uye iwo ekuisa maitiro mumaitiro ezvakatipoteredza. Nepo pfuti yechinyakare ichiswedera neIllumina kutevedzana kwave kushandiswa zvakanyanya muzvidzidzo zvemetagenomic, kuuya kweNanopore nePacBio kutevedzana kwenguva yakareba kuverenga kwakachinja ndima. Nanopore nePacBio tekinoroji inosimudzira yakadzika bioinformatic analys, kunyanya metagenome musangano, kuve nechokwadi magungano anoenderera mberi. Mishumo inoratidza kuti Nanopore-based uye PacBio-based metagenomics yakabudirira kugadzira yakakwana uye yakavharwa mabhakitiriya genomes kubva kune yakaoma microbiomes (Moss, EL, et al., Nature Biotech, 2020). Kubatanidza Nanopore kuverenga neIllumina kuverenga kunopa nzira yekugadziriswa kwekukanganisa, kuderedza kudzika kwaNanopore. Iyi synergistic musanganiswa inokwidziridza masimba epuratifomu yega yega yekuteedzera, ichipa mhinduro yakasimba yekukunda zvingangogumira uye kufambisira mberi iko chaiko uye kuvimbika kwekuongorora kwemetagenomic.
Platform: Nanopore PromethION 48, Illumia uye PacBio Revio
-
16S/18S/ITS Amplicon Sequencing-PacBio
Iwo 16S uye 18S rRNA majini, pamwe neInternal Transcribed Spacer (ITS) dunhu, anoshanda seakakosha mamorekuru ekudhinda zvigunwe nekuda kwekusanganiswa kwawo kwenzvimbo dzakachengetedzwa uye hyper-inosiyana-siyana, zvichivaita maturusi akakosha ekuratidza prokaryotic uye eukaryotic zvipenyu. Kukwidziridzwa uye kutevedzana kweaya matunhu kunopa nzira yekuzviparadzanisa yega yekuongorora kuumbwa kwehutachiona uye kusiyanisa munzvimbo dzakasiyana siyana. Nepo kutevedzana kweIllumina kazhinji kunonangana nematunhu mapfupi akawandisa seV3-V4 ye16S uye ITS1, zvakaratidzwa kuti yepamusoro taxonomic annotation inogoneka nekuteedzera hurefu hwakazara hwe16S, 18S, uye ITS. Iyi nzira yakazara inoguma nezvikamu zvepamusoro zvekutevedzana kwakanyatsorongwa, kuwana chiyero chekugadzirisa chinosvika pakuzivikanwa kwemarudzi. PacBio's Single-Molecule Real-Nguva (SMRT) inoteedzana chikuva inomira pachena nekupa yakanyanya kurongeka yakareba kuverenga (HiFi) iyo inovhara yakazara-yakareba amplicon, ichikwikwidza iko kurongeka kweIllumina kutevedzana. Kugona uku kunobvumira vaongorori kuti vawane mukana usingaenzaniswi - panoramic maonero eiyo genetic landscape. Kufukidzwa kwakawedzerwa kunosimudza zvakanyanya kugadziriswa muzvinyorwa zvezvipenyu, kunyanya mukati menharaunda dzebhakitiriya kana fungal, zvichigonesa kunzwisisa kwakadzama kwekuoma kwehuwandu hwehutachiona.
-
16S/18S/ITS Amplicon Sequencing-NGS
Amplicon kutevedzana neIllumina tekinoroji, yakanangana neiyo 16S, 18S, uye ITS genetic mamaki, inzira ine simba yekufumura phylogeny, taxonomy, uye marudzi kuwanda mukati menharaunda tupukanana. Iyi nzira inosanganisira kuteedzera matunhu akawandisa ekuchengetedza imba mamaki. Pakutanga yakaunzwa se molecular fingerprint byWoeses et almuna 1977, nzira iyi yakashandura microbiome profiling nekugonesa ongororo yemahara. Kuburikidza nekutevedzana kwe16S (mabhakitiriya), 18S (fungi), uye Internal Transcribed Spacer (ITS, fungi), vatsvakurudzi vanogona kuziva kwete chete marudzi akawanda asiwo asingawanzo uye asingazivikanwe. Yakagamuchirwa zvakanyanya sechishandiso chakakosha, amplicon sequencing yave yakakosha mukunzwisisa mutsauko wekuumbwa kwehutachiona munzvimbo dzakasiyana siyana, kusanganisira muromo wemunhu, ura, chituru, nezvimwe.
-
Bhakitiriya uye Fungal Yese Genome Re-Sequencing
Bhakitiriya uye fungal yakazara-genome re-sequencing mapurojekiti akakosha pakusimudzira hutachiona genomics nekugonesa kupedzwa uye kuenzanisa kweiyo microbial genomes. Izvi zvinogonesa kuvirisa engineering, iko optimization yemaindasitiri maitiro, uye kuongorora kwechipiri metabolism nzira. Uyezve, fungal uye bhakitiriya re-kutevedzana kwakakosha pakunzwisisa kuchinjika kwezvakatipoteredza, optimization strains, uye kuburitsa genetic evolution dynamics, ine zvakakura zvinorehwa mukurapa, zvekurima, uye sainzi yezvakatipoteredza.
-
Prokaryotic RNA Sequencing
RNA sequencing inopa simba rakazara profiling yezvese RNA zvinyorwa mukati memasero pasi pemamiriro chaiwo. Iyi tekinoroji yekucheka-kumucheto inoshanda sechishandiso chine simba, kuvheneka akaomesesa gene kutaura profiles, majini zvimiro, uye mamorekuru maitiro ane chekuita neakasiyana maitiro ebhayoloji. Yakagamuchirwa zvakanyanya mukutsvagisa kwakakosha, kuongororwa kwekiriniki, uye kukura kwezvinodhaka, RNA kutevedzana kunopa manzwisisiro mukuomesesa kweserura dynamics uye genetic regulation. Yedu prokaryotic RNA sampuli yekugadzirisa yakagadzirirwa prokaryotic transcriptomes, inosanganisira rRNA kupera uye inotungamira raibhurari kugadzirira.
Platform: Illumina NovaSeq
-
Metatranscriptome Sequencing
Leveraging Illumina sequencing tekinoroji, BMKGENE's metaranscriptome sequencing sevhisi inovhura iyo inochinja yejini kuratidzwa kweakasiyana siyana ehutachiona, kutenderera eukaryotes kune prokaryotes uye mavhairasi, mukati mezvakatipoteredza nharaunda senge ivhu, mvura, gungwa, chituru, uye ura. Sevhisi yedu yakazara inopa simba vaongorori kuti vatarise munharaunda dzakaomarara dzenharaunda 'dzizere gene expression profiles. Kupfuura ongororo yetaxonomic, yedu metaranscriptome sequencing sevhisi inofambisa ongororo mukupfumisa inoshanda, ichivhenekera kujekeswa kwemajini anoratidzwa zvakasiyana uye mabasa awo. Vhura hupfumi hweruzivo rwebhayoloji paunenge uchifamba munzvimbo dzakaoma dzemajeni ekutaura, mutsauko wetaxonomic, uye mashandiro anoita mukati meaya akasiyana niches ezvakatipoteredza.
-
De novo Fungal Genome Assembly
BMKGENE inopa mhinduro dzakasiyana-siyana dzefungal genomes, inotarisira kune zvakasiyana siyana zvekutsvagisa uye inodiwa genome kukwana. Kushandisa pfupi-kuverenga Illumina kutevedzana chete kunobvumira kugadzirwa kweiyo dhizaini genome. Kupfupika-kuverenga uye kureba-kuverenga kutevedzana uchishandisa Nanopore kana Pacbio zvinosanganiswa kune yakawedzera kucheneswa fungal genome ine marefu macontigs. Zvakare, kubatanidza kutevedzana kweHi-C kunowedzera hunyanzvi, kugonesa kuwanikwa kweiyo yakazara chromosome-level genome.
-
Denovo Bacterial Genome Assembly
Isu tinopa yakazara bhakitiriya genome musangano sevhisi, inovimbisa 0 mapundu. Izvi zvinogoneka nekubatanidza matekinoroji ekutevedzana kwenguva refu, akadai seNanopore nePacBio yekusangana uye pfupi-yekuverenga kutevedzana neIllumina yekusimbisa gungano uye kugadzirisa kukanganisa kweONT kuverenga. Sevhisi yedu inopa iyo yakazara bioinformatic workflow kubva kugungano, inoshanda annotation, uye advanced bioinformatic ongororo, kuzadzisa chakanangana nezvinangwa zvekutsvagisa. Iyi sevhisi inogonesa kuvandudzwa kweiyo chaiyo rengedzo genome kune akasiyana genetic uye genomic zvidzidzo. Pamusoro pezvo, inoumba hwaro hwezvishandiso zvakaita se strain optimization, genetic engineering, uye microbial tekinoroji kusimudzira, kuve nechokwadi yakavimbika uye isina gap-isina genomic data yakakosha kusimudzira ruzivo rwesainzi uye biotechnological innovation.
