Nanopore-based Yakazara-kureba Transcriptome Sequencing
Nanopore sequencing inozvisiyanisa kubva kune mamwe mapuratifomu ekutevedzana, pakuti nucleotides inoverengwa zvakananga pasina DNA synthesis uye inogadzira kuverenga kwenguva refu pamakumi e kilobases. Izvi zvinopa simba rakananga kuverenga-kunze kweazere-kureba zvinyorwa uye kugadzirisa zvinonetsa muzvidzidzo zvezvinyorwa.
√ Yakaderera kutevedzana-chaiwo kurerekera
√ Yakazara-kureba kuverenga kwecDNA kune yemajini maitiro ezvidzidzo
√ Dhata shoma inodiwa kuvhara nhamba imwechete yezvinyorwa
√ Kuzivikanwa kweakawanda isoforms pajini
√ Kuratidza kuwanda pane isoform-level
Yakajairika Bioinformatics Pipeline
Dzazvino Dzakabudirira Nyaya neBiomarker Technologies
1. Ongororo yekuenzanisa yezvinyorwa zvehurefu hwakazara zvinoratidza Gnetum luofuense stem development dynamics.
Journal: Frontiers muGenetics
Rakabudiswa: Kurume 2021
Keywords: MinION | Alternative splicing | APA | lncRNA | Zvinyorwa zvinotaridzwa zvakasiyana
Verenga zvizere-zvinyorwa
2. Unidirectional kufamba kwemaRNA madiki kubva panhungirwa kuenda kumidzi mune interspecific heterografts
Nyaya: Zvisikwa Zvirimwa
Rakabudiswa: Ndira 2021
Keywords: Nanopore | Ilumina | hurefu hwakazara mbozha mRNA yekuona
3. Yakazara-yakareba transcriptome kuongororwa kwemidzi yeasparagus inoratidza mamolecular maitiro ekushivirira kwemunyu kunokonzerwa ne arbuscular mycorrhizal fungi.
Journal: Environmental uye Yekuedza Botany
Rakabudiswa: Ndira 2021
Keywords: MinION | Zvinyorwa zvakasiyana-siyana | Transcription factors | Alternative splicing
4. Physiological Characteristic Shanduko uye Yakazara-Kureba Transcriptome yeRose (Rosa chinensis) Midzi neMashizha muKupindura Kusanaya Stress.
Journal: Plant uye Cell Physiology
Rakabudiswa: Gumiguru 2020
Keywords: PromethION | Zvinyorwa zvakasiyana-siyana | Functional prediction | Spliced isoforms | TF uye lncRNA
5. Kuongorora uye kuenzaniswa kwakazara kwePacBio uye nanopore-based RNA sequencing yeArabidopsis transcriptome.
Journal: Plant Method
Rakabudiswa: Kubvumbi 2020
Keywords: GridION | PromethION | PacBio Sequel | Illumina NovaSeq | Genome alignment | Transcript identification | Alternative splicing | SSR | LncRNA | Isoform quantification
Nhau Nezvakakosha ine chinangwa chekugovera nyaya dzichangoburwa dzakabudirira neBiomarker Technologies, kutora zvinyowani zvakabudirira zvesainzi pamwe nemaitiro ane mukurumbira akashandiswa panguva yekudzidza.
Nguva yekutumira: Jan-08-2022