Nanopore yakazara-yakareba transprome

Nanever Transptotome Sequenceme isimba rine simba rekutsakatisa yakazara CDNAS, kuzivisa nenzira kwayo uye kuderedza shanduko yercript. Iyo BMKCLOUD nanopore yakazara-yakareba transptome pombi yakagadzirirwa kuongorora rna-seq data yakagadzirwa neyenzvimbo-yakanaka uye inopa ongororo yekunyora pane ese ari maviri eGene uye transcriptive lever. Mushure memhando yekudzora, izere-kureba-isina-chimeric (FLNC) kutevedzana kwakawanikwa uye kubvumirana kwekubvumirwa kwakasungirirwa genile kubvisa zvinyorwa zvekuregedza. Kubva pane ino tambo set, kutaura kwakazara uye kusiyanisa majini uye zvinyorwa zvinoonekwa uye zvinoshanda zvakanyorwa. Iyo pombi inosanganisirawo imwe polyadenylation (APA) yekuongorora, kufungidzira kwekunyorwa kweLNCET (CDs uye unoshanda unotilation yezvinyorwa.