mRNA-seq (NGS) – De novo

mRNA sequencing inobvumira kunyorwa kwezvose zvinyorwa zvemRNA mumaseru pasi pemamiriro chaiwo uye tekinoroji inoshandiswa zvakanyanya munzvimbo dzakasiyana dzekutsvagisa. Iyo BMKCloud De novo mRNA-seq pombi yakagadzirirwa kuongorora poly-A yakafumiswa yekutevedza maraibhurari kana pasina referensi genome iripo. Iyo pombi inotanga nekutonga kwehutano, inoteverwa nede novotranscript musangano uye unigene set sarudzo. Iyo unigene chimiro chekuongorora inofanotaura kutevedzana kwekodhi (CDS) uye nyore kutevedzana kudzokorora (SSR). Mushure mezvo, kuongororwa kwekutaura kwakasiyana kunowana majini anoratidzwa zvakasiyana (DEGs) pakati pemamiriro akaedzwa, achiteverwa neanotanho ekushanda uye kupfumiswa kweDEGs kuburitsa ruzivo rwebiological.