Hi-C Yakavakirwa Genome Assembly
Hi-C inzira yakagadzirirwa kubata chromosome kumisikidzwa nekubatanidza kuongorora kudhuze-kwakavakirwa kupindirana uye yakakwirira-kuburikidza sequencing. Kusimba kwekudyidzana uku kunofungidzirwa kuve kwakabatana zvisina kunaka nekureba kwemuviri pamakromosomes. Naizvozvo, data reHi-C rinoshandiswa kutungamira kuunganidzwa, kurongeka, uye kutungamira kweakaunganidzwa akateedzana mugwaro rejenome uye kusimudzira iwo pane imwe nhamba yemakromosomes. Iyi tekinoroji inogonesa chromosome-level genome musangano mukushaikwa kwehuwandu-hwakavakirwa genetic mepu. Yese genome imwe inoda Hi-C.
Zvimiro Zvebasa
● Sequencing paIllumina NovaSeq nePE150.
● Kushanda kunoda sampuli dzematishu, panzvimbo pemanucleic acids akatorwa, kuti ayambuke neformaldehyde uye kuchengetedza kudyidzana kweDNA-protein.
● Kuedza kweHi-C kunosanganisira kudziviswa uye kugadziriswa kwemagumo emiti inonamatira nebiotin, inoteverwa nekutenderera kwemagumo asina kujeka anoguma uchichengetedza kupindirana. Iyo DNA inobva yadhonzwa pasi ne streptavidin bead uye inonatswa kuitira kunotevera kugadzirira raibhurari.
Basa Rinobatsira
Mhedziso yeHi-C
(Lieberman-Aiden Et al.,Sayenzi, 2009)
●Kubvisa kudiwa kweGenetic Population Data:Hi-C inotsiva iyo yakakosha ruzivo runodiwa kune contig anchoring.
●High marker density:zvinotungamira kune yakakwira contig anchoring reshiyo pamusoro pe90%.
●Hunyanzvi Hwakawanda uye zvinyorwa zvekushambadzira:BMKGene ine ruzivo rwakakura neanopfuura mazana maviri emakesi eHi-C Genome Assembly kubva kumarudzi zana akasiyana uye akasiyana patents. Anopfuura mazana maviri akaburitswa makesi ane accumulative impact factor inopfuura 2000.
●Yakanyanya hunyanzvi bioinformatics timu:ine-mumba patents uye software copyrights yeHi-C kuyedza uye kuongororwa kwedata, iyo yakazvigadzira yekuona data software inogonesa bhuroka remanyorero kufamba, kudzosera kumashure, kudzoreredza, uye kuitazve.
●Post-Sales Support:Kuzvipira kwedu kunowedzera kupfuura kupera kweprojekiti nemwedzi mitatu mushure mekutengesa sevhisi nguva. Munguva ino, tinopa kutevedza kweprojekiti, rubatsiro rwekugadzirisa matambudziko, uye maQ&A masesheni kugadzirisa chero mibvunzo ine chekuita nemhedzisiro.
●Comprehensive Annotation+
Sevhisi Zvinotsanangurwa
| Kugadzirira raibhurari | Sequencing strategy | Inokurudzirwa kuburitsa data | Kudzora kwehutano |
| Hi-C raibhurari | Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Muenzaniso Zvinodiwa
| Tissue | Mari inodiwa |
| Animal Viscera | ≥ 2 g |
| Tsandanyama dzemhuka | |
| Ropa reMammalian | ≥ 2 mL |
| Huku/Hove Ropa | |
| Chirimwa- Fresh Leaf | ≥ 3 g |
| Cultured Cells | ≥ 1x107 |
| Insect | ≥ 2 g |
Kuyerera Kwebasa Rebasa
Kuedza kugadzira
Muenzaniso wekutumira
Kuvakwa kweraibhurari
Sequencing
Data analysis
Mushure mekutengesa masevhisi
1) Raw data QC
2) Hi-C raibhurari QC: fungidziro yechokwadi Hi-C kudyidzana
3) Hi-C gungano: kuunganidzwa kwe contigs mumapoka, inoteverwa necontig kuronga mukati meboka rega rega uye kugovera contig orientation.
4) Hi-C kuongorora
Hi-C Raibhurari QC - fungidziro yeHi-C inoshanda yekudyidzana pairi
Hi-C Assembly - nhamba
Post-assembly evaluation - heatmap yechiratidzo chekusimba pakati pemabhini
Ongorora kufambira mberi kunofambiswa neBMKGene's Hi-C musangano masevhisi kuburikidza neakaunganidzwa muunganidzwa wezvinyorwa.
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Wang, ZL nevamwe. (2020) 'A Chromosome-Scale Assembly yeAsia Honeybee Apis cerana Genome', Frontiers muGenetics, 11, p. 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.
Zhang, F. nevamwe. (2023) 'Kuratidza shanduko ye tropane alkaloid biosynthesis nekuongorora genomes maviri mumhuri yeSolanaceae', Nature Communications 2023 14: 1, 14 (1), pp. 1-18. doi: 10.1038/s41467-023-37133-4.
Zhang, X. nevamwe. (2020) 'Genomes of the Banyan Tree and Pollinator Wasp Inopa Insights muFig-Wasp Coevolution', Cell, 183(4), pp. 875-889.e17. doi: 10.1016/J.CELL.2020.09.043
