Hi-c based chromatin yekudyidzana
Service maficha
● Sequencing paLillumina Novaseq naPe150.
● Bhaibheri rinoda tishu samples, panzvimbo yekubvisa nuclec acids acids, kuyambuka-chinongedzo ne formaldehyde uye chengetedza iyo DNA-protein kutaurirana.
● Iyo hi-c muedzo unosanganisira kurambidzwa uye nekupedzisira kugadzirisa kweiyo inonamira inopera neBiotin, ichiteverwa nekutenderera kwematokisi yekukonzeresa kupindirana. Iyo DNA yakabva yaburitswa pasi neTrptavidin beads uye yakanatswa yeiyo inotevera raibhurari kugadzirira.
Zvigadzirwa zvebasa
●Yakakwana kurambidza enzyme dhizaini.
●Kuwedzeredza hunyanzvi uye zvinyorwa zvinyorwa:BMKNEE ine yakakura chiitiko ne> 2000 hi-c sequencing mapurojekiti kubva pamakumi masere akasiyana emarudzi uye akasiyana matare. Vanopfuura zana vakaburitswa zviitiko zvine chekuita nechinhu chakabatanidzwa chechinhu chinopfuura 900.
●Anoshamisa-ane hunyanzvi bioinformatics timu:neyekupinda-patents uye software copyrights for hi-c mayedzo uye kuongororwa kwedata uye yekuzvidzora yekuzvidzora yekuona data software.
●Post-Kutengesa Tsigiro:Kuzvipira kwedu kunowedzera kupfuura purojekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa basa nguva. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
●Yakanyatsotaurwa Nyowani: Isu tinoshandisa madhiri akawanda kuti ashande geny gent
Service Rudzidziso
| Raibhurari | Kuongorora zano | Yakakurudzirwa Kubuda kweData | Hi-C Signal resolution |
| Hi-C Library | Illumina Pe150 | Chromatin loop: 150x Tad: 50x | Chromatin loop: 10kb Tad: 40kb |
Sevhisi Yebasa
| Sampuli mhando | Inodiwa huwandu |
| Mhuka tishu | ≥2G |
| Ropa rese | ≥2ml |
| Fungi | ≥1G |
| Zvidyarwa manyoro | 1G / aliquot, 2-4 aciquotes yakakurudzirwa |
| Masero eCustomer | ≥1x107 |
Inosanganisira kuongororwa kunotevera:
● Raw Data QC;
● Mepu uye Hi-C Library QC: Inoshanda Kubatana Pairs uye Kubatana Kuora Kuora (ids);
● genome-yakafara yekubatanidzwa profiti: CIS / Trans leasis uye hi-c yekudyidzana mepu;
● Kuongororwa kweA / B compartment kugoverwa;
● Kuzivikanwa kwematanho uye neChromatin zvishwe;
● Kuongorora kwakasiyana pane 3D chromatin kugadzira zvinhu pakati samples uye inoenderana inoshanda jekisiti yemhando dzakabatana.
Cis uye trans Proportonion kugoverwa
HeatMap yeChromoomaal Kubata pakati samples
Genome-yakakura kugoverwa kweA / B compartments
Genome-yakakura kugoverwa kwe chromatin loops
Kuona kwekuona kweTAD
Ongorora kufambira mberi kwekutsvaga kwakabatanidzwa neBMKGENE's Hi-c sequencing masevhisi kuburikidza nekuunganidzwa kwakasarudzika kwemabhuku.
Meng, T. et Al. .Neuro-oncology, 23 (10), mapeji 1709-1722. Doi: 10.1093 / Neuonc / Noab156.
Xu, l. et al. . "Acta Pharmaceutica Sinoca B, 11 (10), mapeji 3104. Doi: 10.1016 / J.apsb.2021.03.022.
