Mhedzisiro yezviitiko
Zvigadzirwa zvebasa
Takagi et al.,Chinyorwa chirimwa, 2013
●Yakarongeka Bioinformatic Analysis:Kugonesa kufungidzira kwevanhu vakasiyana-siyana, uko kunoratidza kushanduka kwekushanduka kwemarudzi, uye kuburitsa pachena kwakavimbika phylogenet hukama pakati pemarudzi ane kuderedzwa kushanduka-shanduka shanduko uye kufanana kwekushanduka-shanduka
●Inesarudzo yakarongedzwa kuongororwa.
●Yakakura hunyanzvi uye zvinyorwa zvinyorwa.
● Zvakanyanya hunyanzvi bioinformatics timu uye pfupi pfupi kuongorora kutenderera.
● Post-Kutengesa Tsigiro:Kuzvipira kwedu kunowedzera kupfuura purojekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa basa nguva. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Service Rudziro uye Zvinodiwa
| Rudzi rwekutsakatika | Yakakurudzirwa huwandu hwehuwandu | Kuongorora zano | Zvinodiwa Nucleonide |
| Genome geniome sekuseka | ≥ Vanhu makumi matatu, vane ≥ 10 vamwe vanhu vanobva kune yega yega subgroup
| 10x | Kusimbisa: ≥ 1 ng / μl Mari yakazara 30ng Mashoma kana kusasvibiswa kana kusvibiswa |
| Chaiko-locus Amplified Fragment (SLAF) | Tag kudzika: 10x Nhamba yemaki: <400 MB: WGS inokurudzirwa <1GB: 100K tags 1GB > 2GB: 300K tags Max 500K tags | Convertation ≥ 5 ng / μl Mari yakazara ≥ 80 ng NANODROP OD260 / 280 = 1,6-2.5 Agarose gel: hapana kana kushoma kusvibiswa kana kusvibiswa
|
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Service inosanganisira kuongororwa kwehuwandu hwevanhu (phylogenic muti, PCA, huwandu hwekusiyana kwekusiyana, uye kusarudzwa kwehuwandu (uye kusarudzwa kwehurongwa (kusarudza kutsvaira-kusarudzwa kwenzvimbo dzinobatsira). Iyo sevhisi inogona zvakare kusanganisira yakarongeka kuongororwa (semuenzaniso divergence nguva, gene kuyerera).
*Mhedzisiro yakaratidzwa pano zvese kubva kuGenomes yakabudiswa neBMKGE
1.Evolution ongororo ine kuvakwa kweiyo phylogenetic muti, huwandu hwevanhu uye pca zvichibva pane genetic misiyano.
Phylogenic Muti unomiririra taxonomic uye kushanduka hukama pakati pemarudzi ane tateguru.
PCA ine chinangwa chekuona kuswededzwa pakati pevakawanda-huwandu.
Ruzhinji rwevanhu runoratidza kuvapo kweiyo genetically yakasarudzika sub-huwandu maererano neAllele frequency.
Chen, et. al.,Pnas, 2020
2.Sarudza kutsvaira
Sarudza kutsvaira kunoreva maitiro ayo nzvimbo inobatsira inosarudzwa uye nguva zhinji dzevakabatanidzwa masosi anowedzera uye izvo zvemasayiti asina kuderedzwa, zvichikonzera kuderedzwa kwenzvimbo.
Genome-yakafara kuwanikwa pane kusarudza matunhu ekutsvaira anoitwa nekuverenga huwandu hwe genetic index (
Nuleotide kusiana (π)
Tajima's D
Gadziriso index (fst)
Wu, et. al.,MOLECULAR chirimwa, 2018
3.Gene kuyerera
Wu, et. al.,MOLECULAR chirimwa, 2018
4.Demogrical Nhoroondo
Zhang, et. al.,Zvisikwa zvezvisikwa & shanduko, 2021
5.Divergence nguva
Zhang, et. al.,Zvisikwa zvezvisikwa & shanduko, 2021
Ongorora kufambira mberi kwaakafambisa neBmkgene genettution genetical masevhisi kuburikidza nekuunganidzwa kwakasarudzika kweababhuku:
Hassanyar, Ak et al. .Justail International yeMolecular Sayenzi, 24 (7). Doi: 10.390 / ijms24076238.
Chai, J. et Al. .Zoological Research, 2022, Vol. 437 Zviri mugovera 3, mapeji: 469-480, 43 (3), mapeji 469-480. Doi: 10.24272 / J.issn.2095-8137.2022.101.
Han, M. Et Al. .Mberiers mu SPIL SASI, 13, p. 882601. DII: 10.3389 / FPLs.2022.882601 / BIBTEX.
Wang, J. et al. .Horticuluture Rekutsvaga, 9. DOI: 10.1093 / hr / uhac021.
