Eukaryotic Mrna Sequencing-NGS
Zvimiro
● Kurwa kwePoly MrN MRNA isati yabhadharwa
● Inesarudzo yemigwagwa MrNN
● Bioinformatic Ongororo yeGene Expression uye transcript chimiro
Zvakanakira
●Hunyanzvi hwakawanda. Isu tinounza hupfumi hwechiitiko kune chero chirongwa uye takabudirira kupedzisa anopfuura zana emrn-seq mapurojekiti mune dzakasiyana siyana dzekutsvaga nzvimbo.
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwekutakura kwemhando yepamusoro-tsika, kukuvimbisa kuti chirongwa chako chiri mumaoko akavimbika.
●Yakanyatsotaurwa Nyowani: Isu tinoshandisa madhiri akawanda kuti ashande anoratidza maitiro akaratidzwa (gags) uye ita zvinoenderana nekuenzanisa kuongorora. Iyi nzira yakazara inopa nzwisiso mumasero uye molecular maitiro pasi pemhinduro yekunyora, ichivimbisa kuti iwe unonyatso kuziviswa nezvemhedzisiro yako yeProjekiti.
●Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Kuongorora zano | Data yakakurudzirwa | Mhando yekudzora |
| Poly yakafumiswa | Illumina Pe150 DNBSEQ-T7 | 6 -10 GB | Q3085% |
Zvinodiwa Muenzaniso:
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika | |
| Standard Raibhurari | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | Zvidyarwa: Rin≥4.0; Zvemhuka: Rin≥4.5; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
| Kufambisa Raibhurari | ≥ 10 | ≥ 0.2 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | Zvidyarwa: Rin≥4.0; Zvemhuka: Rin≥4.5; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
● Zvirimwa:
Midzi, tsinde kana petal: 450 mg
Shizha kana Mbeu: 300 mg
Michero: 1.2 g
●Mhuka:
Moyo kana mudumbu: 300 mg
Viscera kana uropi: 240 mg
Muswe: 450 mg
Mapfupa, bvudzi kana ganda: 1g
● Arthropood:
Zvipembenene: 6g
Crustacea: 300 mg
● Ropa rese: 1 tube
● Masero: 106 masero
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3 ... ...
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatics
Eukaryotic MrNA Sequencing Ongororo Yekushandisa
Bioinformatics
ØRaw Data Mhando Yekudzora
ØReference genome alignment
ØTranscript Kugadziriswa Kuongorora
ØMashoko ekutaura
ØKusiyanisa kutaura kwezana
ØBasa Rekushanda uye Kufumisa
Reference genome alignment
Data data
Sampula Correlation uye kuongorora kwezvinhu zvakanyorwa zviyedzo
Zvakasiyana-siyana zvinoratidzwa majini (madhiri)
Inoshanda Indiation of DGS
Inoshanda Kufumisa kweDGS
Ongorora kufambira mberi kwakabatana neBMKGECE's Eukaryotic NGRS MrNHER SEQUECE MABHUKU KUNYANYA KUKOSHESA KWEMAHARA eChinyorwa chezvinyorwa.
Huang, l. et al. . 119736. Doi: 10.1016 / J.watres.2023.119736.
Jia, lj et al. . Doi: 10.1016 / j.chom.2023.02.002.
Jin, k. et al. . 884443. DOI: 10.3389 / FPLs.2022.884443 / BIBTEX.
Wen, x. et al. (2022) '
Zhang, Yujie et al. . 101798. DOI: 10.1016 / J.nantod.2023.101798.
