DNBSEQ pre-yakagadzirwa maraibhurari
Features
●Platform:MGI-DNBSEQ-T7
●Sequencing modes:PE150
●Kuendeswa kwemaibhurari eIllumina kuendaMGI:kugonesa kutevedzana kwehuwandu hwe data pamutengo wakaderera.
●Kudzora kunaka kwemaraibhurari kusati kwateedzana.
●Sequencing data QC uye kuendesa:kuendeswa kweQC mushumo uye mbishi data mune fastq fomati mushure me demultiplexing uye kusefa Q30 inoverengwa.
Zvakanakira
●Kusiyana-siyana kweSequencing masevhisi:mutengi angasarudza kutevedzana nemugwagwa kana huwandu hwe data.
●High data output:1500 Gb / nzira
●Kuendeswa kwekuteedzera QC mushumo:nemhando metrics, kurongeka kwedata uye kuita kwese kweprojekiti yekuteedzera.
●Mature sequencing process:nenguva pfupi yekutenderera.
●Rigorous Quality Control: Isu tinoshandisa zvakaomesesa QC zvinodiwa kuti tive nechokwadi chekuunza zvinogara zvemhando yepamusoro mhedzisiro.
Muenzaniso Zvinodiwa
| Nhamba yedata (X) | Concentration (qPCR/nM) | Volume | |
| Partial Lane
| X ≤ 10 Gb | ≥ 1nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ||
| Single Lane | Per Lane | ≥ 1.5 nM / Dziva reraibhurari | ≥ 25 μl / Dziva reraibhurari |
Pamusoro pekutarisa uye huwandu hwakazara, yakakodzera peak pateni inodiwa zvakare.
Ongorora: Kuteedzana kwenzira yemaraibhurari akasiyana-siyana kunoda PhiX spike-in kuti ive nechokwadi chekufona kwakasimba.
Isu tinokurudzira kuendesa pre-yakasanganiswa maraibhurari semienzaniso. Kana iwe uchida BMKGENE kuita raibhurari yekubatanidza, ndapota tarisa kune
raibhurari zvinodiwa pakutevedzana kwechikamu.
Saizi yeraibhurari (Peak mepu)
Peak huru inofanira kuva mukati me300-450 bp.
Maraibhurari anofanirwa kuve nepamusoro pepamusoro, pasina kusvibiswa kweadapter uye pasina maprimer dimers.
Service Workflow
Raibhurari QC mushumo
Chirevo chemhando yeraibhurari inopihwa isati yateedzana, kuongorora huwandu hweraibhurari, uye kupatsanurwa.
Sequencing QC report
Tafura 1. Nhamba dzekuenzanisa data.
| Muenzaniso ID | BMKID | Raw inoverenga | Raw Data (bp) | Kuverenga kwakachena (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Mufananidzo 1. Kugovera kwehutano pamwe chete nekuverenga mumuenzaniso wega wega
Mufananidzo 2. Base content distribution
Mufananidzo 3. Kugovera zvinyorwa zvekuverenga mukuenzanisa data
