Illumina pre-yakagadzirwa maraibhurari
Features
●Mapuratifomu:Illumina NovaSeq 6000 uye NovaSeq X Plus
●Sequencing modes:PE150 uye PE250
●Kudzora kunaka kwemaraibhurari kusati kwateedzana
●Sequencing data QC uye kuendesa:kuendeswa kweQC mushumo uye mbishi data mune fastq fomati mushure me demultiplexing uye kusefa Q30 inoverengwa
Basa Rinobatsira
●Kusiyana-siyana kweSequencing masevhisi:mutengi anogona kusarudza kutevedzana nemugwagwa, kuyerera sero, kana nehuwandu hwe data inodiwa (chikamu mutsara kutevedzana).
●Zvakawanda ruzivo paIllumina sequencing chikuva:nezviuru zvemapurojekiti akavharwa ane marudzi akasiyana-siyana.
●Kuendeswa kwekuteedzera QC mushumo:nemhando metrics, kurongeka kwedata uye kuita kwese kweprojekiti yekuteedzera.
●Mature sequencing process:nenguva pfupi yekutenderera.
●Rigorous Quality Control: Isu tinoshandisa zvakaomesesa QC zvinodiwa kuti tive nechokwadi chekuunza zvinogara zvemhando yepamusoro mhedzisiro.
Sample Platforms
| Platform | Flow Cell | Sequencing mode | Unit | Estimated output |
| NovaSeq X | 10B (nzira 8) | PE150 | Single Lane Partial Lane | 375Gb / Lane |
| 25B (8 nzira) | PE150 | Single Lane Partial Lane | 1000 Gb / Lane | |
| NovaSeq 6000 | SP Flow cell (nzira mbiri) | PE250 | Flow Cell Single Lane Partial Lane | 325-400 M inoverenga / Lane |
| S4 Flow cell (nzira ina) | PE150 | Flow Cell Single Lane Partial Lane | ~ 800 Gb / Lane |
Muenzaniso Zvinodiwa
| Nhamba yedata (X) | Concentration (qPCR/nM) | Volume | |
| Partial Lane Sequencing
| X ≤ 10 Gb | ≥ 1 nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Lane Sequencing | Per Lane | ≥ 1.5 nM / Dziva reraibhurari | ≥ 25 μl / Dziva reraibhurari |
Pamusoro pekutarisa uye huwandu hwakazara, yakakodzera peak pateni inodiwa zvakare.
Ongorora: Kuteedzana kwenzira yemaraibhurari akasiyana-siyana kunoda PhiX spike-in kuti ive nechokwadi chekufona kwakasimba.
Isu tinokurudzira kuendesa pre-yakasanganiswa maraibhurari semienzaniso. Kana iwe uchida BMKGENE kuita raibhurari yekubatanidza, ndapota tarisa kune
zvinodiwa muraibhurari pakutevedzana kwemukoto.
Saizi yeraibhurari (Peak mepu)
Peak huru inofanira kuva mukati me300-450 bp.
Maraibhurari anofanirwa kuve nepamusoro pepamusoro, pasina kusvibiswa kweadapter uye pasina maprimer dimers.
Ndokumbira utaure nesu kana masampula ako akasasangana nekutanga zvinhu zvinodiwa.
Service Workflow
Raibhurari yemhando yekudzora
Sequencing
Data quality control
Kuendesa kweprojekiti
Raibhurari QC mushumo
Chirevo chemhando yeraibhurari inopihwa isati yateedzana, kuongorora huwandu hweraibhurari, uye kupatsanurwa.
Sequencing QC report
Tafura 1. Nhamba dzekuenzanisa data.
| Muenzaniso ID | BMKID | Raw inoverenga | Raw Data (bp) | Kuverenga kwakachena (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Mufananidzo 1. Kugovera kwehutano pamwe chete nekuverenga mumuenzaniso wega wega
Mufananidzo 2. Base content distribution
Mufananidzo 3. Kugovera zvinyorwa zvekuverenga mukuenzanisa data
