Circrna Sequencing-Illumina
Zvimiro
● RRN Flight inoteedzerwa neyekutungamira raibhurari yekugadzirira, inogonesa kuturika-kwakanangana ne data.
● Bioinformatic Workflow inogonesa Circrna kufanotaura uye kutaura kwehuwandu
Zvigadzirwa zvebasa
●Yakanyanya yakazara rnaialiales:Isu tinoshandisa RRN Flight panzvimbo yeLellar RNA Flight muKugadzirira Kwematauro Wedu, Kuverengera Kuti Yakateerana Inga Circna asiwo MrNA uye LNBLAS, Kugonesa kuongororwa kweiyi madhiri aya
●Inesarudzo yekuongorora yemakwikwi endogenus rna (cerna) network: Kupa zvakadzika zvinzwisisike mumasero ekuzvidzora masecurity
●Hunyanzvi hwakawanda.
●Rudzius mhando yekudzoraIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.
● Post-Kutengesa Tsigiro: Kuzvipira kwedu kunowedzera kupfuura projekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa nguva yebasa. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
Mhedzisiro Inodiwa uye Kuendesa
| Raibhurari | Chikuva | Yakakurudzirwa data | Data qc |
| rrna yakadzikira raibhurari raibhurari | Illumina Pe150 | 16-20 GB | Q3085% |
Zvinodiwa Muenzaniso:
Nucleotide:
| Conc. (Ng / μl) | Mari (μg) | Kuchena | Kuvimbika |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 Od260 / 230 = 0.5-2.5 Limited kana hapana protein kana kusvibiswa kweDNA kunoratidzwa pane gel. | RINO6.0; 5.0≥28s / 18s≥1.0; Limited kana Hapana Baseline kukwirira |
● Zvirimwa:
Midzi, tsinde kana petal: 450 mg
Shizha kana Mbeu: 300 mg
Michero: 1.2 g
● Mhuka:
Moyo kana mudumbu: 450 mg
Viscera kana uropi: 240 mg
Muswe: 600 mg
Mapfupa, bvudzi kana ganda: 1.5g
● Arthropood:
Zvipembenene: 9g
Crustacea: 450 mg
● Ropa rese:2 machubhu
● Masero: 106 masero
● Serum uye plasma: 6 ml
Yakakurudzirwa Samplumple Kutumira
Mudziyo: 2 ml centrifige tube (tin foil haina kukurudzirwa)
Sampula Inonyora: Boka + inodzokorora semuenzaniso A1, A2, A3; B1, B2, B3.
Kutumira:
1. Dry-Ice: samples dzinoda kutariswa mumabhegi uye kuvigwa mune yakaoma-ice.
2..
Basa rebasa rinoyerera
Kuedza Dhizaini
Muenzaniso
Rna kubviswa
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Bioinformatics
Circrna kufanotaura: chromoomal kugovera
Zvakasiyana-siyana zvinoratidzwa circrnas - Volcano zano
Kusiyanisa kwakaratidzwa circrnas - hierarchical clustering
Inoshanda yekuvandudza kwedenderedzwa raGirinhwa majini
Ongorora kufambira mberi kwekutsvaga kwakabatanidzwa neBMKGE 'Circrne Sequentecing Services kuburikidza nekuunganidzwa kwakazara kwemabhuku.
Wang, x. et al. . " Doi: 10.1038 / S41388-02-01867-6.
Xia, K. et al. . Doi: 10.1186 / s42483-023-001888-8 / Nights / 6.
Y, h. et al. . ' Doi: 10.21203 / Rs.3.rs-2418311 / v1.
Zhang, Y. et Al. . 109495. DII: 10.1016 / J.Cintimp.2022.109495.
