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Zvigadzirwa

Chromatin immuneckepking sequencing (chip-seq)

Chromatin immuneckerciption sequencing (chip-seq) inofananidzirwa mufananidzo

Chromatin Immuncakeckeption (Chip) inzira iyo inokweretesa antibodies kuti usarudze Ekiremi DNA-BINGING PROPTICS uye yavo inoenderana genomics tarisheni. Kubatanidzwa kwayo naNGS kunoita kuti genome-rese prope yeDNA tarisiro yakabatana neHistone kugadziridzwa, zvinonyorzvera zvinhu, uye imwezve mapuroteni. Iyi nzira ine simba inogonesa kuenzanisa nzvimbo dzekusunga dzinoyambuka mitezo yakasarudzika yemhando dzakasiyana, matishu, kana mamiriro. Chip-cheki's application spancation kubva pakudzidza kudzidzira mutemo uye yekuvandudza nzira kuti isimbe kuita zvechirwere zvechirwere, ichiita chishandiso chakakosha chekunzwisisa nzvimbo dzekutungamira uye kufambira mberi kwekurapa.

Platform: Illumina Nevaaseq

Taura nesu

Nhoroondo dzeBasa

Bioinformatics

Migumisiro

Zvigadzirwa zvebasa

●Yakatangira Bioinformatic Ongororo uye Yakakwana Annotation:Isu tinoshandisa madheti akawanda kuti ashande majini anoenderana nematunhu ematunhu eProtein-dhiza anosunga, anopa ruzivo rwekusungwa uye nemanyorerwo ecolelares pasi pekudyidzana.

●Post-Kutengesa Tsigiro:Kuzvipira kwedu kunowedzera kupfuura purojekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa basa nguva. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.

●Chiitiko chakawandisa:Nekodhi yekutevera yekubudirira kubudirira kupedzisa chip-seq mrojekiti, kambani yedu inounza makore gumi ehunyanzvi hwekunyanzvi patafura. Timu yedu ine hunyanzvi

● Rudzius mhando kutongaIzvi Uku kutarisisa kwekutarisa kunovimbisa kuburitsa kwemhedzisiro yemhando yepamusoro yepamusoro.