De Novo Bactial Generess Gungano
Isu tinopa iyo yakazara yehutachiona yehutachiona gengeny service, kuvimbisa 0 mapundu. Izvi zvinokwanisika nekubatanidza kwenguva refu-kuverenga tekinoroji, senge ninapor uye pacbio yekuGungano uye kupfupi-kuverenga kutsakatika kwesangano rekushandiswa kweOnt kuverenga. Basa redu rinopa iro rakazara bioinformatic Bregly kubva kugungano, rinoshanda rekushanda, uye rakapfuura Bioinformatic Ongororo, kuzadzisa zvinangwa zvekutsvagisa. Iyi sevhisi inogonesa kuvandudza kwechokwadi chirevo cheGenomes zvakasiyana-siyana genetic uye genetsic fundo. Pamusoro pezvo, inoumba hwaro hwekushandisa sekusimudzira kwechiitiko, genetic engineering tekinoroji, inoona yakavimbika uye yekukasira data yakakosha yekufambira mberi kwesainzi neyekutanga ruzivo.
Service maficha
● Nemamwe maviri angangogona kusarudza kubva mukuenderana neiyo degree raunoda yekuzara.
● Draffer genome sarudzo: pfupi-kuverenga kutsakatika neIllumina Nevaaseq pe150.
● Zadzisa 0 gap genome.
● Kuverengera-kuverenga kwenguva refu paN19Pore Promonon 48 kana Pacbio Revio for genome gungano.
● Pfupi-kuverenga kutevedzana kweLillumina novaseq yeGenome kusimbiswa uye kukanganisa kururamisa (nanopore) kana kuburitsa chinongedzo genome.
Zvigadzirwa zvebasa
●Zero-giro genome yakavimbiswa: Izvi zvinokonzerwa nekubatanidzwa kweiyo Illumina kutevedzana nekureba kuverenga kwekuverenga kutevedzana (nanopore kana pacbio).
●Zadzisa Bioinforms Workflow:Izvi zvinosanganisira genome gungano uye kufanotaura kweakawanda genomic zvinhu, basa reGene nhamba, uye genome visoiso dzakadai sec circor.
●Hunyanzvi hwakawanda.
●Post-Kutengesa Tsigiro:Kuzvipira kwedu kunowedzera kupfuura purojekiti kupedzisa nemwedzi mitatu-mushure-yekutengesa basa nguva. Pakati peino nguva, isu tinopa chirongwa chekutevera-up, kugadzirisa dambudziko rekubatsira, uye Q & zvikamu zvekugadzirisa chero mibvunzo zvine chekuita nemhedzisiro.
●Multiple Solencing Strategies inowanikwa:Zvikonzero zvakasiyana zvekutsvagisa uye zvinodiwa zvekutanga zvinyadzo.
Service Rudzidziso
| Sevhisi | Kuongorora zano |
| Draft genome | Illumina Pe150 100X |
| 0 GAP Genome | Nanopore 100x + Illumina pe150 100x Or Pacbio Hifi 30x + Illumina Pe150 100X (Inosarudzwa) |
Zvinodiwa Muenzaniso:
| Concentration (ng / μL) | Mari Yese (μG) | Vhoriyamu (μL) | Od260 / 280 | Od260 / 230 | |
| Pacbio | ≥20 | ≥1.2 | ≥20 | 1.7-2.2.2.2 | ≥1.0 |
| Nanopori | ≥40 | ≥2 | ≥20 | 1.7-2.2.2.2 | 1.0Chi.0 |
| Illumina | ≥1 | ≥0.06 | ≥20 | - | - |
ABBACTRIA: ≥3.5x1010 masero
Basa rebasa rinoyerera
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Inosanganisira kuongororwa kunotevera:
● SEQUENCET DATA YEMAHARA DZESVONDO
● genome gungano
● genome chikamu kuongorora: kufanotaura kweCDS uye akawanda genomic zvinhu
● Inoshanda Annotation ine akawanda madhiri madhiri (enda, Kigg, etc) uye Advanced Databases (Kadhi, VFDB, ETC)
● genome kutadza
Isu tinopa genome mune inowanikwa nyore nyore fepta fomati uye genome annotation faira (GFF).
Gene Aumnation - Enda
Gene Aumnation - Carzy Carbohydrate
Genome kutemi kutaridzika - circos urt
Ongorora kufambira mberi kwakabatana neBmkgee's Bacterial gensle Commy Services kuburikidza neyekuunganidzwa muunganidzwa wezvinyorwa.
Dai, W. et Al. .Justail International yeMolecular Sayenzi, 24 (19), p. 14669. Dii: 10.390 / ijms241914669 / S1.
Kang, Q. et Al. .Kubatanidzwa kwezero, 16 (6), pp. 798-809. Doi: 10.1111 / 1749-4877.12510.
Wang, tt et al. .Chinyorwa cheBiotechnology, 260, mapeji 38-41. Doi: 10.1016 / j.jbiootec.2017.09.001.
Wang, x. et al. . "Mbiru muzvikoro zveMicrobiology, 12, 12, p. 754931. Doi: 10.3389 / ficb.2021.754931 / bibtex.
