Amplicon Sequencing (16s / 18s / its)

Amplicton (16s / 18s / its / its) kutsakatika neiyo nzira yekuongorora microbial kusiyana-siyana uye kwakasiyana-siyana mukati memumwe neumwe samples uye pakati pekuratidzira. Iyo BMKCLOUD amplicon (NGS) PIPELELE inobvumira kuongororwa kwegumi nematanhatu, 18s, its uye akawanda majini anoshanda. Zvinotanga nekuverenga trimming, pakupedzisira kuverenga kuverenga musangano uye mhando yekuongorora, inoteverwa nekubatanidza kweiyo fungidziro yakafanana yekugadzira mashandiro emitero (Otus) inoshandiswa mune zvikamu zvitanhatu zvakasiyana. Taxoniomic antotation inopa ruzivo kuhama yakawanda uye kuumbwa kwemuenzaniso wega wega, ne alpha naBeta Diversity Ongorora pane microbial kusiyana mukati uye pakati pemasampuli, zvichiteerana. Kuongororwa kwakasiyana pakati pemapoka anowana OTUS iyo inosiyana uchishandisa ese parametric uye isiri-paramende bvunzo, nepo kuongorora kwakabatana kunoenderana nemisiyano iyi kune zvakatipoteredza zvinhu. Pakupedzisira, inoshanda gen yakawanda inofungidzirwa zvichibva pane mucherechedzo gene gen yakawanda, ichipa nzwisiso mukuita uye ecology mune imwe neimwe sampuli ..