16s / 18s / ayo amplicon sequencing-ngs
Service maficha
● Sequencing Puratifomu: Illumina Nevaaseq.
● Kuwedzeredza kwenzvimbo pfupi dzegumi nematanhatu, 18s uye kwayo, pakati pezvimwe zvinangwa zveMukati.
● Sarudzo dzinochinja-nyoro dzeAmplicon.
● Pakutanga chirongwa cheProjekti chine akawanda ekuwedzera mafirita.
Zvigadzirwa zvebasa
●YEMAHARA-YEMAHARA:Kukurumidza kuzivikanwa kweiyo microbial composition mune zvakatipoteredza samples.
●Kugadziriswa kwakakwirira: Mune yakanyanya-yakawanda zvikamu mumatare ekunze samples.
●Zvinoshanda zvakanyanya: Dzakasiyana microbial nharaunda zvidzidzo.
●Yakarongedzwa Bioinformatic Analysis.
●Hunyanzvi hwakawanda.
Service Rudzidziso
| Raibhurari | Kuongorora zano | Data yakakurudzirwa |
| Amplicon | Illumina Pe250 | 50/100 / 300k tags (verenga vaviri vaviri) |
Zvinodiwa Nebasa
| Concentration (ng / μL) | Mari yakazara (ng) | Vhoriyamu (μL) |
| ≥1 | ≥200 | ≥20 |
● ivhu / sludge: 1-2g
● Intestinal Zvemukati-Mhuka: 0.5-2g
● Mukati mezvipembenene-zvipembenene: 0.-0.25g
● Chidyara (yakarongedzwa sediment): 0.-0.5g
● Fermentation Broth yakaratidza sediment): 0.-0.5g
● FECES (mhuka hombe): 0.5-2g
● FEECES (mbeva): 3-5Grins
● Pulmonary Alveolar Lavage Fluid: Pepa reFilter
● BASHINAL SWAB: 5-6 Swabs
● Ganda / genital Swab / Saliva / Mirambadzi SERAL STISE / PHARYREAL SWAB / Rectal Swab: 2-3 swabs
● Kumusoro mikanaorganisms: pepa rekufa
● Wakahay Water / Air / Biofilm: Pepa reFilter
● entophytes: 1-2g
● Denda reDzidziso: 0.5-1G
Basa rebasa rinoyerera
Muenzaniso
Raibhurari Kuvaka
Kutevedzana
Kuongororwa kwedata
Mushure mekutengesa-kutengesa masevhisi
Inosanganisira kuongororwa kunotevera:
- Raw Data Mhando Yekudzora
- OTU Clustering / De-ruzha (ASV)
- OTU Annotation
- Alfa Diversity Ongororo: Akawanda Indexs, kusanganisira Shannon, Simpson uye ACE.
- Beta Diversity Analysis
- Inter-Boka Ongororo
- Kuongorora kuongorora: pakati pezvakakomberedza zvinhu uye kusiyana-siyana
- 16s inoshanda Gene kufanotaura
Histogram yeTefonomic Kupararira
Taxoniomic huwandu hunosangana nemepu yekupisa
Alfa Diversity Analysis: Rarefaction Curve
Beta Diversity Analysis: NMDS
Intergrogg Ongororo: Lefse Biomarker kuwanikwa
Ongorora kufambira mberi kwekufambisa neBMKNE's Amplon Sequalton Sequalon Services neIllumina kuburikidza neyakaunganidzwa muunganidzwa wezvinyorwa.
Dong, c. et al. .
Li, Y. et Al. . Dii: 10.1186 / S40168-023-01497-y
Yang, J., fu, Fuu, H. (2022) 'Microbiomes yeLuoregegenerive Live Tsigiro "Lunar Palace 365", micongmotion, 17 (1) 1-20. Doi: 10.1186 / s407-022-00399-0 / nhamba / 8.
Yin, s. et al. . 127678. DOI: 10.1016 / j.biortech.2022.127678.
