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Tasi-nutika rna sequering

Nofofua-Nurticoi Rna sequeding foliga foliga

O le atinaʻeina o le tasi-sela puʻeina ma le masani a le faletusi faletusi fausiaina auala, faʻatasi ai ma le maualuga-alatama, o loʻo teteʻeina o ni suʻesuʻega o le sela i le sela. Lenei malologa faʻatagaina mo le loloto ma sili le auiliiliga o le lavelave o sela lavelave, o le faatoilaloina o tapulaʻa e fesoʻotaʻi ma le averesi o sela i totonu o nei tagata. A o le tasi-sela rna sequeding (scrna-seq) e le mafai ona puipuia lelei, e feagai ma luitau o le a le faia o le sela e tasi-sela. At BMKGene, we address this hurdle by offering single-nucleus RNA sequencing (snRNA-seq) using the state-of-the-art 10X Genomics Chromium technology. O lenei faiga faʻalauteleina na faʻaalia ai le faʻataʻitaʻiga o faʻataʻitaʻiga e mafai ona faʻaaoga i le numera numera i le tasi-sela tulaga.

O le tupua o le onticai o loʻo ausia e ala i le fou 10x conmomicctics chromium chip, o le faʻaalia o le valu-auala microlutidics faiga. I totonu o lenei faiga, gel beds aofia ai bocodes barcodes, primeng, enzymes, ma o le ogatotonu o le Navalit-Sorges Creat-In-Emm Bead Mulimuli mai i le GEM faʻavae, Cell Link ma Barcode faʻasaʻoina e tupu i totonu o le Gema. Mulimuli ane, Mrna Molecules Upgo Lima Risction i CDNA, Faʻapipiʻiina 10x Barcodes ma tulaga ese o le Molecular (Umis). O nei CDNAS o loʻo i ai le faʻavaeina o le faʻatulagaina o le faletusi, faʻafaigofie ai se malosi ma le faʻaaogaina o le faʻafanua o ituaiga o le sela

Tulaga: 10 × chomamoctics chromium ma pulu novaseq tulaga

Faafesootai matou

Auiliiliga faamatalaga

Bioinifofia

Demo taunuuga

Faaosofia lomiga

Polokalame Faateki

O le vavaeeseina o le nuciti ua ausia e le 10 × chomamocs chromium ™, lea e aofia ai le valu-auala Microfludic polokalama ma le faaluaina sopoia. I lenei faiga, o le gel beds ma palu ma priscodes ma prim, enzy ma le tasi ogatotonu o loʻo faʻapipiʻiina i le Navalit-lapoʻa suauʻu o le ale-i-emles (Gem). O le taimi lava e faia ai le gem, o le sela ma le tatalaina o barcodes o loʻo faia i mea taulima taʻitasi. O le Mrna o loʻo ia tuʻuina atu i le CDNA mole ma le 10 × Barcodes ma Umi, o loʻo noatia i le faʻatulagaina o le faletusi.

Foliga

● sauniaina o le tasi-nuckini faʻamalolo mai le faʻaʻaisa tisu

● Fausiaina o Gel Bead-In-Emlilsion (Gem) Mulimuli i CDNA Sythelis

● Uma le pusa i totonu o le kimm ua utaina ma lomiga o lo o aofia ai 4 vaega:

poly (dt) siusiu mo Mrna Pricing ma CDNA Syntheis,

Tulaga ese Molecular faʻailoga (UMI) e faʻasaʻo ai le faʻailoga faʻailo

10x Barcode

Faamaumau le faasologa o le vaega faitau 1 sequening price

Lelei

Nofofua-nucleus rna sequered orseversints o tapulaa o le tasi-sela rna rona sequering, mafai:

● O le faʻaaogaina o faʻataʻitaʻiga faʻaʻaisa ma e le naʻo le faʻatapulaʻaina o faʻataʻitaʻiga fou

● Itiiti popolega o sela faʻaʻaisa pe a faʻatusatusa i le enzymatic togafitiga o sela fou, atagia mai i faʻamatalaga o loʻo i ai

● Leai se manaʻomia mo le aveeseina muamua o mumu toto sela

● Taplimited sela le sili atu

● Tele o faʻataʻitaʻiga e agavaa mo le auiliiliga, e aofia ai lavelave, o le 'eseʻese o aano o loʻo fuafua i le sela faʻataua

Faʻataʻitaʻiga e le mafai ona auiliiliina e le tasi-sela rna sequening ma e agavaa mo le tasi nuticoi rna:

Sela / tisu	Mafuaaga
Aveesea Frozen Frozen	Le mafai ona maua ni suiga fou poʻo ni faʻalapotopotoga umi
Maso sela, megakaryocyte, gaʻo ...	E tele naua le sela
Ate ...	E gagau e gagau, le mafai ona iloa e tasi sela nofofua
Neuron sela, faiiʻai ...	Sili atu le maaleale, faigofie ona popole, o le a suia ai le faasologa iuga
Pancreas, Thyroid ...	Mauoa i ni encogenamen enzymes, afaina ai le gaosiga o le tasi sela taofia

Tasi-nucleus vs nofofua-sela

Tasi-nuculeus	Tasi-sela
Le faatapulaaina sela	Cell diameter: 10-40 μm
O mea e mafai ona faʻaʻaisa	O le mea e tatau ona fou solo
Maualalo le popole o sela aisa	Enzyme Togafitiga e ono mafua ai le lotoa o le sela popolega
Leai se sela mumu e tatau ona aveese	O sela mumu e tatau ona aveeseina
O le faaniukilia foliga mai o le le fiafia	O le sela atoa e foliga mai o le leai

Faamatalaga fautuaina

Faʻataʻitaʻiga Manaʻoga

Faletusi

SUʻESUʻEGA FAʻATASI

Faamatalaga Fautuaina

Lelei Puleaina

Manu tisu ≥ 200 mg

Totoina tisu ≥ 400 mg

10x conmictics sn CDNA Faletusi

Imumlina pex50

100k pe faitau i le sela

(100-200 GB)

700-1200 nuciti / μl ma nuciti o le faamaoni e maitauina i lalo o le microscope

Mo nisi faʻamatalaga o loʻo i le faʻataʻitaʻiga o aʻoaʻoga o le faʻatonuga ma tautua galuega, faʻamolemole lagona le saoloto e talanoa i le aBMKGEne tomai

Auaunaga galue

Talu ai: Laau / manu atoa

Le isi: Menme-lautele Faalapotopotoga Iloiloga

E aofia ai le auiliiliga lenei:

● Faʻamaoniga o sela: Aofai o sela, Gene Evection, o loʻo faʻailoa mai ai o sela, RNA mole ma faʻaaliga

● I totonu o le faʻataʻitaʻiga faʻataʻitaʻiga:

Cel Clisterting ma Cluster le iloa

FAʻAALIGA FAʻAALIGA FAʻAALIGA: Faʻailoaina o le DEGS i le fusuʻaga

O le mea moni o le faʻaaogaina ma le faʻauiga o le faʻailoga

● Suʻesuʻega-Vaega Faʻatinoga:

Tuʻufaʻatasiga o faʻamatalaga

Faʻaliga Faʻafanua Suʻesuʻega: Faʻailoaina o le DEGS i vaega

O le faʻamatalaga faʻapitoa ma le faʻauiga o le vaega o kulupu

● Alualu i Luma:

Suʻega o le Cell Cycle

Auiliiliga iloiloga

CODE Consinge Accessing Product (Cellphotageb)

Gread seti le auiliiliga auiliiliga (GSEA)

Inner-faʻataʻitaʻiga auiliiliga

Cel Clisterting:

Faʻafanua Faʻaliga Faʻamatalaga: Cluster DEGS

Suesuega Suesuega

Faʻatusalia faʻamatalaga auiliiliga: Vaega DEGS

Iloiloga lautele:

Faamatalaga auiliili:

COCK TAE SILI:

Saili i alualu i luma faʻafaigofie e le tasi-nuclene-nucucus rna o le ronanceing tautua e 10x chromium i nei faʻaaliga lomiga:

Wang, l. et al. (2021) 'E toʻatasi-Cell Dranscriptotic Ose auiliiliga o loʻo faʻaalia ai le laufanua o le lungTaualumaga a le National Academy o Saienisi o le Iunaite Setete o Amerika, 118 (2), i. e20055590118. DoI: 10.1073 / PNAS.2005590118

Zhel, h. et al. (2022) 'O le psybal tulafono faʻatonu mo le DYSREEDE TAMAITI MA LE NUMONICE MIABOBOCE' ISHANDE O LE MINIDODELLuma i le fogaula, 13, p. 879824. DOI: 10.3389 / BIMMU.2022.879824 / Bibtex.

Tian, H. et al. (2023) 'tasi-sela Transporttoma encovers heterogenely ma le puipuia o le leukocytes pe a uma le tui ma le lecounded ed)),Vaivai, 566, i. 739238. DOI: 10.1016 / J. I.723.739238.

Ioe, Y. Et al. (2023) 'Photoddictimic togafitiga faʻaleleia atili le iʻuga o le Internepoint Checkpoint Caphiciator e ala i le toe fausia o le Aneti-Bomorty i tagata gasegase ma le Kortaric Canes',Faletupe Kander, 26 (5), i. 798-813. DoI: 10.1007 / S10120-023-01409-x / metrics.