Fa'asologa o Transcriptome Fa'asologa atoa e fa'avae i Nanopore
O le fa'asologa o le Nanopore e 'ese'ese mai isi fa'asologa fa'asologa, ona o le nucleotides e faitau sa'o e aunoa ma le fa'asologa o DNA ma fa'atupuina le faitau umi i le sefulu kilobases. O lenei mea e faʻamalosia ai le faitau saʻo o faʻamaumauga atoa ma faʻafeiloaʻi luitau i suʻesuʻega faʻasalalau.
√ Fa'aituau fa'asologa fa'asologa maualalo
√ Faitauga atoa o le cDNA mo su'esu'ega fa'atulagaina o kene
√ Fa'aitiitia fa'amaumauga e mana'omia e aofia ai le numera tutusa o tusitusiga
√ Fa'ailoaina o le tele o isoforms ile kene
√ Fa'amatalaga fa'atusa ile tulaga fa'aisoform
Ole Pipeline masani ole Bioinformatics
Mata'upu Fa'amanuiaina Fa'afou ma Biomarker Technologies
1. O su'esu'ega fa'atusatusa o tusitusiga fa'asolo atoa e fa'aalia ai le malosi o le atina'e o le Gnetum luofuense stem.
Tusitala: Frontiers in Genetics
Lolomiina: Mati 2021
upu autu: MiniON | Su'ega isi | APA | lncRNA | Fa'aliliuga fa'aalia eseese
Faitau Tusi atoa
2. Fa'agasolo fa'atasi o tama'i RNA mai lala e o'o i a'a i fa'ato'aga fa'atasi.
Tusitala: La'au Natura
Lolomiina: Ian. 2021
upu autu: Nanopore | Ilumina | su'esu'ega mRNA feavea'i atoa
3. O le su'esu'ega atoa o a'a asparagus e fa'aalia ai le mole mole o le fa'apalepale o le masima e mafua mai i sigi arbuscular mycorrhizal.
Tusitala: Siosiomaga ma Fa'ata'ita'i Fa'ato'aga
Lolomiina: Ian. 2021
upu autu: MiniON | Fa'aliliuga fa'aalia eseese | Fa'amatalaga fa'aliliuga | Su'ega fa'aopoopo
4. Suiga o Uiga Fa'aletino ma le Transcriptome atoa ole Rose (Rosa chinensis) A'a ma Laulaau e Tali atu ai i Mauga Lala.
Tusitala: Plant and Cell Physiology
Fa'asalalau: Oke. 2020
upu autu: PromethION | Fa'aliliuga fa'aalia eseese | Va'aiga galue | Isoforms so'o | TF ma lncRNA
5. Suʻesuʻega ma faʻatusatusaga faʻatusatusa o le PacBio ma le nanopore-based RNA sequencing o le Arabidopsis transcriptome
Tusitala: Metotia Toto
Lolomiina: Ape. 2020
upu autu: GridION | Folafolaga | PacBio Fa'asologa | Illumina NovaSeq | Genome alignment | Fa'amatalaga fa'amatalaga | Su'ega isi | SSR | LncRNA | Isoform quantification
Tala Fou ma Mea Taua e fa'atatau i le fa'asoaina atu o mataupu fa'amanuiaina lata mai ma Biomarker Technologies, fa'amauina o taunu'uga fa'asaienisi fa'apea fo'i ma auala iloga na fa'aogaina i le taimi o su'esu'ega.
Taimi meli: Ian-08-2022