Le umi o le le-le-coungeding-istumini
Tulaga lelei
●Faaopoopo auiliiliga o le Mrna ma Lncrna: I le tuʻufaʻatasia o le aofaʻiga o tala a Mrna ma le suʻesuʻega o Lncrna ma a latou sini, e mafai ona maua se vaʻaiga loloto o le auala sili ona taliaina.
●Lautele tomai faapitoa: Na aumaia e le ma au o le oa o le poto masani i galuega uma, ma se faʻamaumauga o le faʻagaioiga o le 23,000 faʻataʻitaʻiga i le BMK SPRING CHARSND ITULAU ERSE.
●Malo le lelei: Matou te faʻatinoina vaega o loʻo faʻaaogaina i vaega uma, mai le faʻataʻitaʻiga ma le faletusi tapenaga i se lipine ma bioninictoctics. O lenei manatu faʻapitoa e mautinoa ai le tuʻuina atu o le sili ona lelei-tulaga maualuga.
●Meli-faatau lagolago: O la tatou tautinoga na sosoʻo atu i tua atu o le poloketi ua maeʻa le 3-masina ina ua faʻatau atu auaunaga. I le taimi lea, matou te ofoina atu ai le faʻagaioiga e mulimulitaʻia, faʻafitauli fesoasoani, ma Q & ma ni sauniga e faʻailoa ai soʻo se fesili e fesoʻotaʻi ma faʻaiuga.
Faataʻitaʻi Manaʻoga ma Tuʻuina atu
| Faletusi | Tulaga | Fautuaina faamatalaga | Faamatalaga QC |
| Na faaitiitia RRNA | Imumlina pex50 | 10-16 GB | Q30085% |
Faatau:
| Pepa. (Ng / μl) | Aofaiga (μg) | Amio mama | Sao |
| ≥ 80 | ≥ 0.8 | Od260 / 280 = 1.7-2.5 OD260 / 230 = 0.5-2.5 Faʻatapulaʻa pe leai se porotini poʻo le DNA o loʻo faʻaalia o le gel. | Rinway6.0; 5.0028s / 18s≥1.0; faatapulaaina pe leai se totogi masani |
● Laau:
Root, stem po o pesega: 450 mg
Lau poʻo le fatu: 300 mg
Fua: 1.2 g
● manu:
Loto po o le mafaufau: 450 mg
Viscera poʻo le faiʻai: 240 mg
Maso: 600 mg
Ivi, lauulu po o le paʻu: 1.5g
● Arthropods:
Iniseti: 9g
Crustagram: 450 mg
● toto atoa:2 tubes
● sela: 106 sela
● serum ma plasma: 6 ml
Fautuaina le Aiatusi Tuufaatasi
Container: 2 ml centrifrifoge tube (tin fortil e le fautuaina)
Faataʻitaʻi igoa: Vaega + Remactate eg A1, A2, A3; B1, b2, b3.
Uta:
1. Meaola-Ice: faʻataʻitaʻiga e tatau ona teu i ato ma tanu i le mama-aisa.
2. Rnastable tubes: rna faʻataʻitaʻiga e mafai ona mago i le rna stabilization tube (eg rnastable®) ma lafo i le vevela vevela.
Auaunaga galue
Tofotofoga Faatosinaga
Faataʻitaʻiga Faafou
Rna overction
Fausiaina o le Faletusi
Liu
Faamatalaga o faamatalaga
A maeʻa-faʻatau auaunaga
Bioinifofia
Eseese Grey Freet (DEGS) Iloiloga
Aofaʻi o lncrna faʻaaliga - faʻaputuputu
Faalauiloaina o lncrna sini tupuaga
Sociate Mrna ma Lncrna tulaga auiliiliga - Circos Patcos PLOTO (CORTCE CROD o Mrna ma Iner Cicrlce o Lncrena)
Saili ni alualu i luma faʻafaigofie e Bmkgene's lncrne o le masani ai auaunaga e ala i se faʻavasega aoina o lomiga.
Ji, H. et al. (2020) 'Faʻaiuga, aoga vavalo, ma Key lncrna faʻamaonia o le malulu o le manava-fesoʻotaʻi o lncyas 2021: 1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 (1, 10 DOI: 10.1038 / S41598-020-574511-7.
Jia, z. et al. (2021) 'O manatu tuʻufaʻatasia o loʻo faʻaalia ai le faʻaaogaina o mea totino mo se cyhv-3-fulipaina masani carp incep', 12, p. 687151. DOI: 10.3389 / BIMMU.2021.687151 / Bibtex.
Wang, xj et al. (2022) 'O le tele o aʻoaʻoga-faʻavae faʻavae muamua o le tauvaga o le rna tulafono faʻatonutonu o fesoʻotaʻiga RNA o loʻo i totonu o le Courk Ling 904865. Doi: 10.3389 / Fonc.2022.904865 / BIBTX.
Xiao, l. et al. (2020) 'Genera Diresection of the Grown Combrimprossion Network i lalo o le Potoulus i Willus', 18 (4), pp. 101). DOI: 10.1111 / PBI.13270.
Zhel, h. et al. (2022) 'O le tulafono faʻavae a le lalolagi mo le dyssivated gelegatared ma le masani ai metabolin metabolic o sela', 13, p. 879824. DOI: 10.3389 / BIMMU.2022.879824 / Bibtex.
