De Novo fingal conana
BMKGENE E ofoina atu fofo Verale o Frangal Pramemes, o le faʻatulagaina o suʻesuʻega eseese o suʻesuʻega ma manaʻomia le atoatoa atoatoa. Faʻaaogaina le puʻupuʻu-faitau o le Fusulia sequenie o le faʻatagaina o le augatupulaga o se ata mataga. Puʻupuʻu-faitau ma le umi-faitau sequeding faʻaaogaina Nanopore poʻo Pacbio o loʻo tuʻufaʻatasia mo le sili atu le toe faʻaleleia o le fusuʻaga. E le gata i lea, tuʻufaʻatasia o le hi-c requeding o loʻo faʻaleleia atili ai le faʻamalosiʻau o agavaʻa, e mafai ona maua se mea e maua ai se chromomos.
Tautua o foliga
Faatasi ai ma le tolu filifiliga e mafai ona filifili mai le faʻalagolago i le tulaga manaʻomia o le atoatoa atoatoa:
● Faʻasalaga le filifiliga a le Actime: Faʻamalosi-faitau sequening ma imoturi novaseq pe150.
● fiafia lelei filifiliga filifiliga:
GENUME SUʻESUʻEGA: O Yumumina Novaseq Pe150.
Granement Concripment: Pacbio Regio (HIFI faitauga) po o le Nanopore prometitote 48.
● Chromososo-tulaga o le fiva angame:
GENUME SUʻESUʻEGA: O Yumumina Novaseq Pe150.
Granement Concripment: Pacbio Regio (HIFI faitauga) po o le Nanopore prometitote 48.
Fetau i totonu o le CRI- CF
Tulaga lelei
●Tele ni mea e maua ai: Mo eseese suʻesuʻega sini ma manaʻoga o le atoatoa atoatoa
●Faʻatumu le BIININSICATICTS Workflow:E aofia ai ma le tele o aʻoaʻoga ma le vavalo o le tele o elemene o elemene, o le galuega a Grenomentation, ma 'aila.
●Lautele tomai faapitoa: E tusa ma le 12,000 miliona microblial promemers, matou te faʻatupesofi i le atoaga o le poto masani, o se auiliiliga auiliiliga, o le aofaʻi atoatoa, ma lelei pou metotia lagolago, ma lelei pou lagolago, ma lelei pou lagolago, ma lelei pou tau-faʻatau-faʻatau lagolago.
●Lagolago lagolago:O la matou tautinoga na sosoʻo atu i tua atu o le poloketi na maeʻa le 3-masina ina ua faʻatau atu auaunaga. I le taimi lea, matou te ofoina atu ai le faʻagaioiga e mulimulitaʻia, faʻafitauli fesoasoani, ma Q & ma ni sauniga e faʻailoa ai soʻo se fesili e fesoʻotaʻi ma faʻaiuga.
Auaunaga Mataupu Faavae
| Tautua | SUʻESUʻEGA FAʻATASI | Lelei Puleaina |
| Ata ata | Issumina P150 100x | Q30085% |
| Lelei plaome | Gentame Group: Imeligina P150 50 x Fonotaga: Pacbio Hifi 30x po o le Nanopore 100x | Oloa n50 ≥1M1 (Pacbio Uncilellular) Oloa n50 ≥2MB (Itt Unclelular) Oloa N50 ≥500kb (isi) |
| Chromosome-tulaga pronome | Gentame Group: Imeligina P150 50 x Fonotaga: Pacbio Hifi 30x po o le Nanopore 100x Talofa-c fono 100x | Onsign Anchoring Raitio> 90%
|
Tautua Tautua
| Manatu (ng / μl) | Aofai aofaiga (μg) | Voluma (μl) | OD260 / 280 | OD260 / 230 | |
| Pasebio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nanopore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Immumina | ≥1 | ≥0.06 | ≥20 | - | - |
Unicelular fingus: ≥3.5x1010 sela
Macro fingus: ≥10 g
Auaunaga galue
Faataʻitaʻiga Faafou
Fausiaina o le Faletusi
Liu
Faamatalaga o faamatalaga
A maeʻa-faʻatau auaunaga
E aofia ai le auiliiliga lenei:
GENMEN SOUCY:
- Sequetions faamatalaga lelei pulea
- Fuafuaina Fuafuaina: Lautele, Heterozygosaty, faifai pea elemene
Lelei Cleare Fono Faigofie:
- Sequetions faamatalaga lelei pulea
- De novoFaiaoga
- O le auiliiliga o le vaega o le vaega: Valoaga o CDS ma le tele o elemene elemene
- O le faʻauiga o le faʻamatalaga ma le tele o faʻamaumauga lautele (alu, kegg, etc.) ma alualu i luma datably (card, vfdb, vfdb, vtc, vfdb, vfdb, vfdb, vfdb, vfdb, vfdB
Talofa-c fono:
- HI-I le faletusi suesuega.
- O loʻo feʻelaʻe faʻatele
- Iloiloga o le HIF-C fono: Faʻavae i luga o faʻasino i le le mautonu ma le vevela faʻafanua
Granement Sound: K-Malosiaga tufatufaina
Genera Concripment: Gene Homologius Annolocust (NR Database)
Genera Concreen Commen: Galuega Faʻatinoina o le ANT le le Anonose (alu)
Saili ni alualu i luma faʻafaigofie e le Bmkgene Commoune Communine Communine i se faʻavasega aoina o lomiga.
Hoo, J. et al. (2023) 'tuʻufaʻatasia omi o le Ocormal of the MedicAL Medicroom Innothous Ono sefuluoprous i lalo o tulaga i lalo o le Soundment',BMC Togafitiga, 24 (1), i. 1-12. DOI: 10.1186 / S12864-023-09656-Z / Ata / Ata / Ata / Ata / Ata / Ata / Ata /
Lu, l. et al. (2023) 'O le Maota Malie o loʻo faʻaalia ai le evolusione ma pathogenic mechanisms o le saito o le saito o mata o le gehogenO le tala fatu, 11 (2), pp. 405-416. DoI: 10.1016 / J.CJ.022.07.024.
Zhang, H. Et al. (2023) 'Prayme Punaoa mo le fa Clariorian Aiaiga na mafua ai le Doller Deady i luga o le Dit Asfgraft',Totoina o le faʻamaʻi, 107 (3), pp. 929-934. DoI: 10.1094 / PDIS-08-22-1521-a
Zhang, ss et al. (2023) 'Generatic ma le Molecular Teugatupe o le Tetrapolar Matting System i le Edead Geadware BandoSa',Lipoti a TungI, 9 (10), i. 959. Doi: 10.3390 / Jof9100959 / S1.
