TGuide Smart Blood/Cell/Tissue RNA Kit
Numera fa'amaumauga / afifiina
| Pusi. leai | ID | Nu. o sauniuniga |
| 4995043(papa) | DP661-E | 96 |
| 4993551(kesi) | DP661-DE | 48 |
Ta'iala filifilia mo le TGuide S16
| Nuclei Acid | Ituaiga Fa'ata'ita'i | Igoa Oloa | Tele o le afifiina (sauniuniga) kesi/ ipu | Pusi nu. Kesi/papa |
| DNA | Tisu Manu | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | La'au ma Fatu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Eleele/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Gel & PCR oloa | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Toto Atoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Mago, ma isi | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Toto/Sela/Tisu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | La'au ma Fatu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Suavai e leai se sela (Serum, ma isi) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Folasaga o oloa
Faʻamama le maualuga-maea, maualuga-mama, maualuga le aofaʻi o le RNA mai le toto / sela / gaʻo.
Leai ni vaila'au o'ona pei ole phenol/chloroform o lo'o iai
O le RNA na maua e maualuga le mama ma e mafai ona faʻaoga saʻo mo le suʻeina o pu, faʻasologa maualuga ma isi suʻega.
Vaega
Super faigofie e faʻaaoga
Leai se isi galuega paipa.
E mafai ona fetu'una'i le voluma elution pe a mana'omia.
O le faʻapipiʻiina ma le faʻaogaina e manaʻomia ai sina aʻoaʻoga. Faatasi ai ma polokalame na muai faʻapipiʻiina, tatala le pusa, filifili le protocol ma faʻatautaia lau suʻega. Polokalama ua saunia-e-fa'aoga, ma fa'apitoa fo'i.
Reagents mua'i utaina ma fa'afetaui mea fa'aaoga fa'aaoga.
O le tagofiaina o vailaʻau e faʻaumatia i le tulaga sili e faʻamautinoa ai le saogalemu o le gaioiga.
Fa'aaliga Fa'aaliga
Agilent 2100 na faʻaaogaina mo le faʻamaonia o le faʻamaoni.
Fa'ata'ita'iga puna: CD-1 isumu
Tulaga fa'ata'ita'iga: toto fou fou
Fa'ata'ita'iga tele: 200 μl
Fa'ata'ita'iga taunu'uga: o le aofa'i o le RNA na maua mai le toto e TGuide S16 fa'apipi'iina i le reagent 4993551 e maua ai se fua lelei ma maualuga le mama, ma e mafai ona fa'afetaui mana'oga o lalo ifo.
fa'asologa maualuga.
Fa'ata'ita'iga puna: CD-1 isumu
Tuuina atu le voluma: 1 μl. O le ate na faʻafefe 5 taimi ma utaina.
Akaraose gel fa'asalaina: 1%. Electrophoresis mo le 20 min i le 6 V / cm;
M: TIANGEN Maka III 1-4: fatu 5-8:
ate 9-12: māmā 13-16: fatuga'o
O fa'ata'ita'iga muamua e lua o mea ta'itasi na maua mai e le pusa fa'ata'ita'i e fa'avae i luga ole koluma, ma o mea mulimuli e lua na otometi lava ona aveese mai e TGuide S16.
Fa'ata'ita'iga taunu'uga: o le RNA aano o manu e otometi lava ona aveese mai e TGuide S16 fa'aauupegaina i le reagent 4993551 e maualuga le fua ma le mama, lea e tutusa ma le RNA na maua mai e le fa'asologa o le fa'avaeina o le fa'aputuga. Ole mama e tutusa lava, ma OD260/OD280 ile 2.0~2.1 ma le OD260/OD230>2.0. I le fa'ai'uga, e mafai ona fa'aogaina le TGuide S16 e su'e mai ai le RNA atoa mai aano o manu e suitulaga i le vaifofo fa'avae fa'avae.
