Eukaryotic mRNA Sequencing-NGS
Vaega
● Pu'eina le poly mRNA a'o le'i saunia le faletusi
● Fa'atonuga ole faletusi mRNA saunia ina ia mafai ai ona maua fa'amaumauga fa'asologa fa'apitoa
● Bioinformatic suʻesuʻega o faʻamatalaga o kenera ma faʻasologa o tusitusiga
Tulaga lelei
●Tomai Tele: O la matou 'au na fa'atautaia le silia ma le 600,000 fa'ata'ita'iga i le BMKgene, fa'ata'amilo i ituaiga fa'ata'ita'iga 'ese'ese e pei o aganu'u sela, a'au, ma sua o le tino. Matou te aumaia le tamaoaiga o le poto masani i galuega uma ma ua maeʻa manuia i luga ole 100,000 mRNA-Seq poloketi i vaega eseese o suʻesuʻega.
●Pulea lelei: Matou te fa'atinoina le fa'atonuga autu i la'asaga uma, mai le fa'ata'ita'iga ma tapenaga faletusi i le fa'asologa ma bioinformatics. O lenei mata'ituina ma'oti e fa'amautinoa ai le tu'uina atu o fa'ai'uga e sili ona lelei, fa'amautinoa o lo'o i lima fa'atuatuaina lau poloketi.
●Fa'amatalaga Atoa: Matou te fa'aogaina le tele o fa'amaumauga e fa'ailoa ai le fa'aigoa o le Differentially Expressed Genes (DEGs) ma fa'atino su'esu'ega fa'atamaoaigaina tutusa. O lenei faiga fa'apitoa e maua ai fa'amatalaga i le telefoni feavea'i ma mole mole o lo'o fa'avaeina le tali transcriptome, fa'amautinoa o lo'o e malamalama atoatoa i taunu'uga o lau poloketi.
●Lagolago Post-Fa'atau: O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o galuega faʻatasi ma le 3-masina pe a maeʻa le faʻatau atu taimi o auaunaga. I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.
Fa'ata'ita'iga Mana'oga ma le Tu'uina atu
| Faletusi | Fuafuaga fa'asologa | Fa'amatalaga fautuaina | Puleaina lelei |
| Poly A fa'atamaoaigaina | Illumina PE150 DNSSEQ-T7 | 6 -10 Gb | Q30≥85% |
Fa'ata'ita'iga Mana'oga:
Nucleotides:
| Conc.(ng/μl) | Aofa'i (μg) | Mama | Fa'amaoni | |
| Faletusi masani | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Fa'atapula'a pe leai se polotini po'o le fa'aleagaina o DNA o lo'o fa'aalia ile gel. | Mo laau toto: RIN≥4.0; Mo manu: RIN≥4.5; 5.0≥28S/18S≥1.0; fa'atapula'a pe leai se maualuga maualuga |
| Faletusi Fa'atonu | ≥ 10 | ≥ 0.2 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Fa'atapula'a pe leai se polotini po'o le fa'aleagaina o DNA o lo'o fa'aalia ile gel. | Mo laau toto: RIN≥4.0; Mo manu: RIN≥4.5; 5.0≥28S/18S≥1.0; fa'atapula'a pe leai se maualuga maualuga |
● La'au:
A'a, Po'o Po'o Petal: 450 mg
Laulaau po'o Fatu: 300 mg
Fualaau: 1.2 g
●Manu:
FOTO po'o le Tonu: 300 mg
Viscera po'o Fai'ai: 240 mg
Muso: 450 mg
Ivi, Laulu po'o Pa'u: 1g
● Arthropods:
Iniseti: 6g
Crustacea: 300 mg
● toto atoa: 1 paipa
● sela: 106 sela
Fautuaina Fa'ata'ita'iga Tu'uina atu
Containe: 2 ml centrifuge tube (E le fautuaina le pepa apa)
Fa'ailoga fa'ata'ita'iga: Vaega+fa'atusa eg A1, A2, A3; B1, B2, B3... ...
utaina:
1. Fa'amamago-aisa: Fa'ata'ita'iga e mana'omia ona fa'apipi'i i taga ma tanu i le aisa-mago.
2. RNAs table tubes: RNA samples e mafai ona fa'amago ile RNA stabilization tube(eg RNAstable®) ma lafo ile potu vevela.
Auala o Galuega Au'aunaga
Fa'ata'ita'iga mamanu
Fa'ata'ita'iga tu'uina atu
RNA su'ega
Fausia faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
Bioinformatics
Eukaryotic mRNA su'esu'ega fa'asologa o galuega
Bioinformatics
ØPulea lelei fa'amatalaga mata'utia
ØFa'asinoga genome alignment
ØSu'esu'ega fa'asologa o tusitusiga
ØFa'amatalaga fa'atusa
ØSu'esu'ega fa'amatalaga eseese
ØFa'amatalaga galuega ma fa'atamaoaigaina
Fa'asinoga Genome Alignment
Tulaga Fa'amatalaga
Fa'ata'ita'iga feso'ota'iga ma su'esu'ega o fa'asologa o mea ola
Genes ua Fa'aalia Eseese (DEGs)
Fa'amatalaga Fa'atino o DEGs
Fa'atamaoaigaina Galuega ole DEGs
Su'esu'e le aga'iga'i i luma na fa'afaigofieina e le BMKGene's eukaryotic NGS mRNA sequencing services e ala i le aoina o fa'asalalauga.
Huang, L. et al. (2023) 'Triclosan ma triclocarban fa'avaivaia le malosi o le manogi o i'a auro e ala i le taofiofia o le iloa o le manogi, fa'alavelave le fa'aliliuina o fa'ailo, ma le fa'alavelaveina o fa'amatalaga fa'amatalaga', Su'esu'e Suavai, 233, i. 119736. doi: 10.1016/J.WATRES.2023.119736.
Jia, LJ et al. (2023) 'Aspergillus fumigatus e faoa le p11 tagata e toe fa'asa'o phagosomes o lo'o iai fungal i le ala e le fa'aleagaina', Cell Host & Microbe, 31(3), itulau 373-388.e10. doi: 10.1016/J.CHOM.2023.02.002.
Jin, K. et al. (2022) 'TCP Transcription Factors Aʻafia i le Atinaʻeina o Atinaʻe o Ma Bamboo (Dendrocalamus latiflorus Munro)', Frontiers in Plant Science, 13, p. 884443. doi: 10.3389/FPLS.2022.884443/BIBTEX.
Wen, X. et al. (2022) 'O le Chrysanthemum lavandulifolium genome ma le molecular mechanism o lo'o i lalo o ituaiga capitulum eseese', Horticulture Research, 9. doi: 10.1093/HR/UHAB022.
Zhang, Yujie et al. (2023) 'O se cascade nanoreactor mo le faʻaleleia o le sonodynamic togafitiga i le kanesa colorectal e ala i le synergistic ROS faʻateleina ma le autophagy poloka', Nano Today, 49, p. 101798. doi: 10.1016/J.NANTOD.2023.101798.
