Dnbseq muamua-faia faletusi
Foliga
●Tulaga:MGI-DNBSEQ-T7
●Sequentioding modes:Pe150
●Faʻaliliuina o le Yelumpona Faletupe iMgi:Faʻamaoniga o le faʻasologa o faʻamatalaga maualuga o loʻo i le tau maualalo.
●O le lelei pulea o faletusi ae e te leʻi faia.
●Sequetions faʻamaumauga QC ma tilivaina:Faʻalauiloaina o QC Lipoti ma Raw faʻamaumauga i le Freequction format pe a maeʻa le demoupleplexing ma faʻamamaina Q30 faitauina.
Lelei
●Vaosi o le faasologa o tautua:E mafai e le tagata faʻatau ona filifili e faʻasologa e le lane poʻo le aofaʻi o faʻamaumauga.
●Maualuga faʻamatalaga faʻamatalaga:1500 GB / Lane
●Tuuina atu o le faasologa QC lipoti:ma le lelei metrics, o faʻamatalaga saʻo ma le aotelega o le galuega o le faʻasologa o le faʻasologa.
●Mamaina le faasologa o le faasologa:ma le puʻupuʻu taimi-taimi.
●Malo le lelei: Matou te faʻatinoina le faʻaaogaina o le QC manaʻoga e faʻamaonia ai le tuʻuina atu o le sili ona lelei-tulaga maualuga.
Faʻataʻitaʻiga Manaʻoga
| Faamatalaga o faamatalaga (x) | Faapipiiina (QPCR / NM) | Fua | |
| Vaega vaega
| X ≤ 10 gb | ≥ 1nm | ≥ 25 μl |
| 10 GB <x ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| 50 GB <x ≤ 100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> 100 GB | ≥ 4 nm | ||
| Tasi laina | I le lane | ≥ 1.5 nm / faletusi vaitaele | ≥ 25 μl / faletusi vaitaele |
E le gata i lea e tuʻusaʻo ma le aofaʻi atoa, o se pa talafeagai o mamanu e manaʻomia foi.
Faʻamatalaga: La Line o loʻo faʻaopopoina o le maualalo o eseese falefaigaluega e manaʻomia ai le pix spike-i totonu e faʻamautinoaina le tele o faʻavae.
Matou te fautuaina le lafoina muamua-faʻapipiʻi faletusi o ni faʻataʻitaʻiga. Afai e te manaʻomia BMKGEne e faia le vaitaʻele o le faletusi, faʻamolemole tagaʻi i le
O mea e manaʻomia e le autusi a le fale.
Lautele (Peak Faafanua)
Pito i luga e tatau ona i totonu o le 300-450 BP.
E tatau ona i ai i faletusi se tasi pito sili ona taua, leai se fetuunaiga o loʻo aʻafia ma leai se faʻailoga muamua.
Tautua galuega
Faletusi QC Lipoti
O le lipoti i luga o le lelei o le faletusi o loʻo tuʻuina atu aʻo leʻi maeʻa le lisi, faʻaaogaina aofaʻi o le Tusitala, ma faʻavasegaina.
Sequeting QC Lipoti
Laulau 1. Fuainumera i luga o le faasologa o faamatalaga.
| Faʻataʻitaʻiga ID | Bmkid | E faitau | Raw faamatalaga (BP) | Mama faitau (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | Bmk_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,4115,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Ata 1. Tuʻuina atu le vaega o loʻo faitauina i faʻataʻitaʻiga taʻitasi
Ata 2. Fualatu o le PASP
Ata 3. Fasiotifaina o faitauga Mataupu i le lisi o faamatalaga
