-
Kufananiza Genomics
Kuyerekeza ma genomics kumakhudzanso kuwunika ndi kufananiza magawo onse a ma genome ndi kapangidwe kake pakati pa mitundu yosiyanasiyana. Gawoli likufuna kuwulula za kusinthika kwa zamoyo, kuzindikira momwe majini amagwirira ntchito, ndikuwunikira njira zoyendetsera ma genetic pozindikira momwe zimasungidwira kapena zotsatizana ndi zinthu zamoyo zosiyanasiyana. Kafukufuku wofananira wa ma genomics amaphatikizapo kusanthula monga mabanja a majini, kakulidwe ka chisinthiko, zochitika zamitundu yonse, komanso zotsatira za kukakamiza kosankha.
-
Evolutionary Genetics
Pulatifomu yowunikira anthu komanso kusintha kwa majini imakhazikitsidwa potengera zomwe zachitika mu gulu la BMK R&D kwazaka zambiri. Ndi chida chosavuta kugwiritsa ntchito makamaka kwa ofufuza omwe sachita zazikulu mu bioinformatics. Pulatifomuyi imathandizira kusanthula koyambira kokhudzana ndi kusinthika kwa ma genetics kuphatikiza kupanga mitengo ya phylogenetic, kusanthula kwa kulumikizana, kuwunika kwamitundu yosiyanasiyana, kusanthula kosankha, kusanthula ubale, PCA, kusanthula kamangidwe ka anthu, ndi zina zambiri.
-
Hi-C Based Genome Assembly
Hi-C ndi njira yomwe idapangidwa kuti ijambule masinthidwe a chromosome pophatikiza kuyesa kuyanjana kochokera kufupi ndi kutsata kwapamwamba. Kukula kwa kuyanjana uku kumakhulupirira kuti kumalumikizidwa moyipa ndi kutalika kwa ma chromosome. Chifukwa chake, data ya Hi-C imagwiritsidwa ntchito kutsogolera kusanja, kuyitanitsa, ndi kalozera wa ma kromozomu omwe asonkhanitsidwa mu janimu yokonzekera ndikulumikiza ma chromosome angapo. Ukadaulo uwu umathandizira kuphatikiza kwa ma chromosome genome popanda mapu otengera kuchuluka kwa anthu. Genome iliyonse imafunikira Hi-C.
-
Zomera / Zinyama za De Novo Genome Sequencing
De Novokutsatizana kumatanthauza kupanga ma genome amtundu wonse pogwiritsa ntchito umisiri wotsatizana popanda ma genome. Kuyamba ndi kufalikira kwa kutsatizana kwa m'badwo wachitatu, wokhala ndi kuwerenga kwanthawi yayitali, kwathandizira kwambiri kuphatikiza kwa ma genome pakuwonjezera kuphatikizika pakati pa zowerenga. Kupititsa patsogolo kumeneku kumakhala koyenera makamaka polimbana ndi ma genome ovuta, monga omwe amawonetsa heterozygosity yapamwamba, chiwerengero chachikulu cha zigawo zobwerezabwereza, ma polyploids, ndi zigawo zomwe zimakhala ndi zinthu zobwerezabwereza, zomwe zili mu GC zachilendo, kapena zovuta kwambiri zomwe nthawi zambiri zimasonkhanitsidwa bwino pogwiritsa ntchito masanjidwe afupipafupi. yekha.
Yankho lathu loyimitsa limodzi limapereka ntchito zophatikizika zotsatizana komanso kusanthula kwa bioinformatic komwe kumapereka mtundu wapamwamba kwambiri wa de novo assembled genome. Kufufuza koyambirira kwa ma genome ndi Illumina kumapereka kuyerekezera kwa kukula ndi zovuta za genome, ndipo chidziwitsochi chimagwiritsidwa ntchito kutsogolera gawo lotsatira la kuwerengera kwanthawi yayitali ndi PacBio HiFi, kutsatiridwa ndindi novomsonkhano wa contigs. Kugwiritsiridwa ntchito kotsatira kwa msonkhano wa HiC kumathandizira kukhazikika kwa ma contigs ku genome, kupeza msonkhano wa chromosome. Pomaliza, ma genome amatsimikiziridwa ndi kulosera kwa jini komanso potsata ma jini owonetsedwa, kutengera ma transcriptomes okhala ndi zowerengera zazifupi komanso zazitali.
-
Sequencing ya Human Whole Exome
Human Whole exome sequencing (hWES) imadziwika kuti ndiyo njira yotsika mtengo komanso yamphamvu yolondolera masinthidwe omwe amayambitsa matenda. Ngakhale amangopanga pafupifupi 1.7% ya ma genome onse, ma exons amagwira ntchito yofunika kwambiri powonetsa momwe mapuloteni amagwirira ntchito. Makamaka, m'matupi aumunthu, kupitilira 85% ya masinthidwe okhudzana ndi matenda amawonekera mkati mwa zigawo zama protein. BMKGENE imapereka ntchito yokwanira komanso yosinthika ya anthu onse amtundu wa exome yokhala ndi njira ziwiri zojambulira za exon zomwe zilipo kuti zikwaniritse zolinga zosiyanasiyana za kafukufuku.
-
Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
Kujambula kwapamwamba kwambiri, makamaka kwa anthu akuluakulu, ndi sitepe yofunika kwambiri pa maphunziro a chibadwa cha chibadwa ndipo imapereka maziko a majini ogwiritsira ntchito majini, kusanthula kwachisinthiko, ndi zina zotero.Kuchepetsa Kuyimilira kwa Genome Sequencing (RRGS)nthawi zambiri amagwiritsidwa ntchito m'maphunzirowa kuti achepetse mtengo wotsatirira pachitsanzo chilichonse ndikusunga bwino pakupezeka kwa ma genetic. RRGS imakwaniritsa izi pogaya DNA yokhala ndi ma enzymes oletsa ndikuyang'ana kagawo kakang'ono kachidutswa, potero amangotsata kachigawo kakang'ono ka genome. Mwanjira zosiyanasiyana za RRGS, Specific-Locus Amplified Fragment Sequencing (SLAF) ndi njira yosinthira makonda komanso yapamwamba kwambiri. Njira iyi, yopangidwa paokha ndi BMKGene, imakulitsa ma enzyme oletsa ntchito iliyonse. Izi zimatsimikizira kupangidwa kwa ma tag ochulukira a SLAF (magawo 400-500 bps a genome akutsatiridwa) omwe amagawidwa mofanana mumtundu wonse ndikupewa madera obwerezabwereza, kutsimikizira kupezedwa kwabwino kwa cholembera.
-
malaibulale opangidwa kale ndi Illumina
Tekinoloje yotsatizana ya Illumina, yozikidwa pa Sequencing by Synthesis (SBS), ndiukadaulo wapadziko lonse wa NGS, womwe umapangitsa kuti pakhale 90% yazotsatizana zapadziko lonse lapansi. Mfundo ya SBS imaphatikizapo kujambula zoziziritsa zotchedwa fulorosenti zotchedwa reversible terminators pamene dNTP iliyonse ikuwonjezedwa, ndipo kenako imadulidwa kuti ilole kuphatikizidwa kwa maziko otsatirawa. Ndi ma dNTP onse anayi osinthika omangika otsekera omwe amapezeka mumayendedwe aliwonse, mpikisano wachilengedwe umachepetsa kukondera. Ukadaulo wosunthikawu umathandizira malaibulale owerengeka okha komanso ophatikizidwa awiriawiri, popereka ma genomic applications. Illumina sequencing amatha kuchita bwino kwambiri komanso kulondola kwake kumayiyika ngati mwala wapangodya pa kafukufuku wa genomics, kupatsa mphamvu asayansi kuti adziwe zovuta za ma genomes mwatsatanetsatane komanso mosayerekezeka.
Ntchito yathu yotsatirira laibulale yopangidwa kale imathandizira makasitomala kukonzekera malaibulale otsatizana kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, malaibulale 10x, pakati pa ena). Pambuyo pake, malaibulalewa amatha kutumizidwa kumalo athu otsatizana kuti aziwongolera komanso kusanja pamapulatifomu a Illumina.
