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  • Metagenomic Sequencing -NGS

    Metagenomic Sequencing -NGS

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    Metagenome ndi mndandanda wazinthu zonse zopezeka m'magulu osiyanasiyana a zamoyo, monga chilengedwe ndi anthu. Lili ndi ma genomes omwe amalimidwa komanso osalimidwa. Shotgun metagenomic sequencing ndi NGS imathandizira kusanthula kwamitundu yodabwitsayi yophatikizidwa mu zitsanzo zachilengedwe popereka zambiri kuposa mbiri ya taxonomic, kuperekanso chidziwitso chaching'ono cha mitundu yosiyanasiyana ya zamoyo, kuchuluka kwa mphamvu, ndi kuchuluka kwa kuchuluka kwa anthu. Kupitilira pa maphunziro a taxonomic, shotgun metagenomics imaperekanso mawonekedwe owoneka bwino a genomics, omwe amathandizira kuwunika kwa majini osungidwa ndi maudindo awo okhazikika pazachilengedwe. Pomaliza, kukhazikitsidwa kwa maukonde olumikizana pakati pa ma genetic ndi zinthu zachilengedwe kumathandizira kumvetsetsa kwathunthu kuyanjana kwapakati pamagulu ang'onoang'ono ndi chilengedwe chawo. Pomaliza, kutsatizana kwa metagenomic kumakhala ngati chida chofunikira kwambiri powunikira zovuta zamitundu yosiyanasiyana yamagulu ang'onoang'ono amitundu yosiyanasiyana, ndikuwunikira ubale wosiyanasiyana pakati pa majini ndi chilengedwe mkati mwazachilengedwe zovutazi.

    Mapulatifomu: Illumina NovaSeq ndi DNBSEQ-T7

  • Metagenomic Sequencing-TGS

    Metagenomic Sequencing-TGS

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    Metagenome ndi mndandanda wazinthu zamtundu wamagulu osakanikirana a zamoyo, monga chilengedwe ndi anthu. Lili ndi ma genomes omwe amalimidwa komanso osalimidwa. Kutsatizana kwa Metagenomic kumathandizira kusanthula kwamitundu yodabwitsayi yophatikizidwa mu zitsanzo zachilengedwe popereka zambiri kuposa mbiri ya taxonomic. Imaperekanso mawonekedwe ogwiritsira ntchito ma genomics poyang'ana majini osungidwa ndi maudindo awo pazochitika zachilengedwe. Ngakhale kuti mfuti zachikhalidwe zimayandikira ndi kutsatizana kwa Illumina zakhala zikugwiritsidwa ntchito kwambiri m'maphunziro a metagenomic, kubwera kwa Nanopore ndi PacBio kuwerengera kwanthawi yayitali kwasintha gawoli. Ukadaulo wa Nanopore ndi PacBio umakulitsa kusanthula kwa bioinformatic, makamaka msonkhano wa metagenome, kuwonetsetsa kuti misonkhano ipitirire. Malipoti akuwonetsa kuti Nanopore-based and PacBio-based metagenomics apanga bwino ma genome athunthu ndi otsekedwa a bakiteriya kuchokera ku ma microbiomes ovuta (Moss, EL, et al., Nature Biotech, 2020). Kuphatikiza mawerengedwe a Nanopore ndi mawerengedwe a Illumina kumapereka njira yowongolera zolakwika, kuchepetsa kulondola kwa Nanopore. Kuphatikizana kophatikizana kumeneku kumawonjezera mphamvu za nsanja iliyonse yotsatizana, kumapereka yankho lamphamvu kuti athe kuthana ndi malire omwe angakhalepo ndikupititsa patsogolo kulondola komanso kudalirika kwa kusanthula kwa metagenomic.

    Platform: Nanopore PromethION 48, Illumia ndi PacBio Revio

  • Whole genome bisulfite sequencing (WGBS)

    Whole genome bisulfite sequencing (WGBS)

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    Whole Genome Bisulfite Sequencing (WGBS) imayima ngati njira yodziwika bwino ya golide yowunikira mozama DNA methylation, makamaka malo achisanu mu cytosine (5-mC), wowongolera kwambiri wamafotokozedwe amtundu ndi zochitika zama cell. Mfundo yomwe ili pansi pa WGBS imaphatikizapo chithandizo cha bisulfite, kupangitsa kutembenuka kwa ma cytosine osatulutsidwa kukhala uracil (C mpaka U), ndikusiya ma methylated cytosine osasinthika. Njirayi imapereka njira imodzi yokha, yomwe imalola ofufuza kuti afufuze mozama za methylome ndikupeza njira zachilendo za methylation zomwe zimagwirizanitsidwa ndi mikhalidwe yosiyanasiyana, makamaka khansa. Pogwiritsa ntchito WGBS, asayansi atha kupeza zidziwitso zosayerekezeka za mawonekedwe a genome-wide methylation, ndikupereka kumvetsetsa kwapang'onopang'ono kwa njira za epigenetic zomwe zimayambitsa njira zosiyanasiyana zamoyo ndi matenda.

  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)

    Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)

    ATAC-seq ndi njira yotsatirira kwambiri yomwe imagwiritsidwa ntchito posanthula kupezeka kwa chromatin mu genome. Kugwiritsiridwa ntchito kumapereka kumvetsetsa kwakuya kwa machitidwe ovuta a epigenetic control pa gene expression. Njirayi imagwiritsa ntchito hyperactive Tn5 transposase kuti igawane ndikuyika magawo otseguka a chromatin poyika ma adapter otsatizana. Kukulitsa kwa PCR kotsatira kumapangitsa kuti pakhale laibulale yotsatizana, yomwe imalola kuzindikirika kwathunthu kwa zigawo zotseguka za chromatin pansi pamikhalidwe yanthawi yanthawi. ATAC-seq imapereka mawonekedwe athunthu amitundu yofikira ya chromatin, mosiyana ndi njira zomwe zimangoyang'ana pamasamba omangirira zinthu kapena zigawo zina zosinthidwa ndi histone. Posanjikiza zigawo zotseguka za chromatin, ATAC-seq imawulula madera omwe atha kukhala ndi katsatidwe kokhazikika komanso masamba omwe angamangirize, zomwe zimapereka chidziwitso chofunikira pakusinthika kwamtundu wa jini kudera lonselo.

  • 16S/18S/ITS Amplicon Sequencing-PacBio

    16S/18S/ITS Amplicon Sequencing-PacBio

    Mitundu ya 16S ndi 18S rRNA, pamodzi ndi dera la Internal Transcribed Spacer (ITS), amagwira ntchito ngati zolembera zala zala zala chifukwa cha kuphatikiza kwawo madera otetezedwa komanso osinthika kwambiri, kuwapanga kukhala zida zamtengo wapatali zozindikiritsa zamoyo za prokaryotic ndi eukaryotic. Kukulitsa ndi kutsatizana kwa maderawa kumapereka njira yopanda kudzipatula yofufuzira kapangidwe ka tizilombo tating'onoting'ono komanso kusiyanasiyana kwachilengedwe. Ngakhale kutsatizana kwa Illumina nthawi zambiri kumayang'ana zigawo zazifupi ngati V3-V4 ya 16S ndi ITS1, zawonetsedwa kuti ndemanga zapamwamba za taxonomic zimatheka potsata kutalika kwa 16S, 18S, ndi ITS. Njira yonseyi imapangitsa kuti chiwerengero cha anthu chikhale chodziwika bwino, zomwe zimapangitsa kuti anthu azitha kuzindikira zamoyo. PacBio's Single-Molecule Real-Time (SMRT) yotsatirira nsanja imawonekera popereka zowerengera zazitali zolondola (HiFi) zomwe zimaphimba ma amplicons aatali, kupikisana ndi kulondola kwa Illumina. Kutha kumeneku kumalola ofufuza kuti apeze mwayi wosayerekezeka - mawonekedwe owoneka bwino a mawonekedwe amtundu. Kufalikira kokulirapo kumakweza kwambiri chigamulo m'zambiri zamitundu, makamaka m'magulu a bakiteriya kapena mafangasi, zomwe zimathandizira kumvetsetsa mozama za zovuta zamitundumitundu.

  • 16S/18S/ITS Amplicon Sequencing-NGS

    16S/18S/ITS Amplicon Sequencing-NGS

    Kutsatizana kwa Amplicon ndi ukadaulo wa Illumina, makamaka kutsata zolembera zamtundu wa 16S, 18S, ndi ITS, ndi njira yamphamvu yovumbulutsira kuchuluka kwa phylogeny, taxonomy, ndi mitundu yamitundu m'magulu ang'onoang'ono. Njira iyi imaphatikizapo kutsata zigawo zosasinthika za zolembera zanyumba. Poyambirira adawonetsedwa ngati chala chala chala ndiMavuto et almu 1977, njira iyi yasintha mbiri ya microbiome popangitsa kusanthula kopanda kudzipatula. Kupyolera mu kutsatizana kwa 16S (mabakiteriya), 18S (bowa), ndi Internal Transcribed Spacer (ITS, bowa), ofufuza amatha kuzindikira osati mitundu yambirimbiri komanso yosowa komanso yosadziwika. Chomwe chimatengedwa ngati chida chofunikira kwambiri, kutsatizana kwa amplicon kwathandizira kuzindikira mitundu yosiyanasiyana ya tizilombo tosiyanasiyana m'malo osiyanasiyana, kuphatikiza pakamwa pa munthu, matumbo, chopondapo, ndi kupitirira apo.

  • Mabakiteriya ndi fungal Yense ya Genome Yotsatizana

    Mabakiteriya ndi fungal Yense ya Genome Yotsatizana

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    Ntchito zotsatizananso za mabakiteriya ndi mafangasi amtundu wonse ndizofunikira kwambiri pakupititsa patsogolo ma genomics a microbial genomics pothandizira kukwaniritsidwa ndi kufananiza ma genome a microbial. Izi zimathandizira uinjiniya wa fermentation, kukhathamiritsa kwa njira zamafakitale, komanso kufufuza njira zachiwiri za metabolism. Kuphatikiza apo, kutsatizananso kwa mafangasi ndi mabakiteriya ndikofunikira kuti timvetsetse kusintha kwa chilengedwe, kukhathamiritsa zovuta, komanso kuwulula zakusintha kwa majini, zomwe zimakhudza kwambiri zamankhwala, ulimi, ndi sayansi ya chilengedwe.

  • PacBio-Full-length 16S/18S/ITS Amplicon Sequencing

    PacBio-Full-length 16S/18S/ITS Amplicon Sequencing

    Pulatifomu ya Amplicon (16S/18S/ITS) imapangidwa ndi zaka zambiri zakusanthula kwama projekiti osiyanasiyana, omwe amakhala ndi kusanthula koyambira komanso kusanthula kwamunthu payekha: kusanthula kofunikira kumakhudza kusanthula kwakukulu kwa kafukufuku waposachedwa wa tizilombo tating'onoting'ono, zowunikira ndizolemera komanso zomveka, ndi zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a polojekiti; Zomwe zili pakuwunika kwamunthu ndizosiyanasiyana. Zitsanzo zitha kusankhidwa ndipo magawo atha kukhazikitsidwa mosinthika malinga ndi lipoti lowunikira komanso cholinga cha kafukufuku, kuti mukwaniritse zomwe mukufuna. Mawindo opangira makina, osavuta komanso ofulumira.

  • PacBio-Full-length Transcriptome (Non-Reference)

    PacBio-Full-length Transcriptome (Non-Reference)

    Kutenga data ya Pacific Bioscience (PacBio) Isoform yotsatirira monga zolowetsa, Pulogalamuyi imatha kuzindikira masanjidwe aatali athunthu (popanda kuphatikiza). Popanga mapu a mautali athunthu motsutsana ndi ma genome, zolembedwa zimatha kukongoletsedwa ndi majini odziwika, zolembedwa, zigawo zolembera, ndi zina zotere. Pankhaniyi, kuzindikirika kolondola kwa mapangidwe a mRNA, monga kuphatikizika kwina, ndi zina, zitha kukwaniritsidwa. Kusanthula kophatikizana ndi data ya NGS transcriptome sequencing kumathandizira kutanthauzira momveka bwino komanso kuchulukitsidwa kolondola kwambiri pofotokozera pamlingo wa transcript, zomwe zimapindulitsa kwambiri kufotokozera kosiyanasiyana komanso kusanthula magwiridwe antchito.

  • Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)

    Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)

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    Reduced Representation Bisulfite Sequencing (RRBS) yatuluka ngati njira yotsika mtengo komanso yothandiza ku Whole Genome Bisulfite Sequencing (WGBS) mu kafukufuku wa DNA methylation. Ngakhale WGBS imapereka chidziwitso chokwanira powunika ma genome onse pamalingaliro amodzi, mtengo wake wokwera ukhoza kukhala wolepheretsa. RRBS imachepetsa vutoli mwa kusanthula mosankha gawo loyimira ma genome. Njirayi imadalira kulemeretsa kwa zigawo zolemera za zilumba za CpG ndi MspI cleavage yotsatiridwa ndi kusankha kukula kwa zidutswa za 200-500/600 bps. Chifukwa chake, madera okhawo omwe ali pafupi ndi zilumba za CpG amatsatiridwa, pomwe omwe ali ndi zilumba zakutali za CpG amachotsedwa pakuwunika. Njirayi, yophatikizidwa ndi kutsata kwa bisulfite, imalola kuzindikira kwapamwamba kwa DNA methylation, ndi njira yotsatizana, PE150, imayang'ana makamaka kumapeto kwa zoyikapo m'malo mwapakati, kukulitsa luso la methylation profiling. RRBS ndi chida chamtengo wapatali chomwe chimathandizira kufufuza kwa DNA methylation kopanda mtengo komanso kupititsa patsogolo chidziwitso cha njira za epigenetic.

  • Kutsata kwa Prokaryotic RNA

    Kutsata kwa Prokaryotic RNA

    Kutsatizana kwa RNA kumapatsa mphamvu kulongosola kwatsatanetsatane kwa zolembedwa zonse za RNA m'maselo pansi pamikhalidwe inayake. Ukadaulo wotsogolawu umagwira ntchito ngati chida champhamvu, kuwulula mbiri yakale ya jini, kapangidwe ka majini, ndi njira zama cell zomwe zimagwirizanitsidwa ndi mitundu yosiyanasiyana yachilengedwe. Kutengera kwambiri pakufufuza kofunikira, kuwunika kwachipatala, komanso chitukuko chamankhwala, kutsatizana kwa RNA kumapereka chidziwitso pazovuta zama cellular dynamics komanso kuwongolera majini. Kukonzekera kwathu kwa prokaryotic RNA kumapangidwira zolemba za prokaryotic, zomwe zikuphatikiza kuchepa kwa rRNA ndikukonzekera laibulale yowongolera.

    Platform: Illumina NovaSeq

  • Kutsata kwa Metatranscriptome

    Kutsata kwa Metatranscriptome

    Tekinoloje yotsatizana ya Illumina, BMKGENE's metaranscriptome sequencing service ikuwulula mawonekedwe amtundu wamitundu yosiyanasiyana ya tizilombo tating'onoting'ono tosiyanasiyana, totengera ma eukaryotes mpaka ma prokaryotes ndi ma virus, m'malo achilengedwe monga dothi, madzi, nyanja, chimbudzi, ndi matumbo. Ntchito yathu yokwanira imapatsa mphamvu ofufuza kuti afufuze mbiri yamitundu yonse yamagulu ocheperako. Kupitilira kusanthula kwa taxonomic, ntchito yathu yotsatizana ya metatranscriptome imathandizira kufufuza pakulemeretsa kwa magwiridwe antchito, kuwunikira ma jini owonetsedwa mosiyanasiyana ndi maudindo awo. Dziwani zambiri zazachilengedwe mukamayang'ana mawonekedwe ovuta a ma gene, mitundu yosiyanasiyana ya taxonomic, komanso magwiridwe antchito mkati mwamitundu yosiyanasiyana yazachilengedwe.

  • Ndi Novo Fungal Genome Assembly

    Ndi Novo Fungal Genome Assembly

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    BMKGENE imapereka mayankho osunthika a ma genomes a mafangasi, yothandiza pazosowa zosiyanasiyana za kafukufuku komanso kukwanira kwa ma genome. Kugwiritsa ntchito kusanja kwa Illumina kwakanthawi kochepa kumalola kupanga ma genome. Mawerengedwe afupikitsa komanso owerengera nthawi yayitali pogwiritsa ntchito Nanopore kapena Pacbio amaphatikizidwa kuti apange genome yoyengedwa bwino kwambiri yokhala ndi ma contig ataliatali. Kuphatikiza apo, kuphatikiza kutsatizana kwa Hi-C kumapititsa patsogolo luso, ndikupangitsa kuti munthu athe kupeza mtundu wathunthu wamtundu wa chromosome.

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