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Metagenomic Sequencing -NGS
Metagenome ndi mndandanda wazinthu zonse zopezeka m'magulu osiyanasiyana a zamoyo, monga chilengedwe ndi anthu. Lili ndi ma genomes omwe amalimidwa komanso osalimidwa. Shotgun metagenomic sequencing ndi NGS imathandizira kusanthula kwamitundu yodabwitsayi yophatikizidwa mu zitsanzo zachilengedwe popereka zambiri kuposa mbiri ya taxonomic, kuperekanso chidziwitso chaching'ono cha mitundu yosiyanasiyana ya zamoyo, kuchuluka kwa mphamvu, ndi kuchuluka kwa kuchuluka kwa anthu. Kupitilira pa maphunziro a taxonomic, shotgun metagenomics imaperekanso mawonekedwe owoneka bwino a genomics, omwe amathandizira kuwunika kwa majini osungidwa ndi maudindo awo okhazikika pazachilengedwe. Pomaliza, kukhazikitsidwa kwa maukonde olumikizana pakati pa ma genetic ndi zinthu zachilengedwe kumathandizira kumvetsetsa kwathunthu kuyanjana kwapakati pamagulu ang'onoang'ono ndi chilengedwe chawo. Pomaliza, kutsatizana kwa metagenomic kumakhala ngati chida chofunikira kwambiri powunikira zovuta zamitundu yosiyanasiyana yamagulu ang'onoang'ono amitundu yosiyanasiyana, ndikuwunikira ubale wosiyanasiyana pakati pa majini ndi chilengedwe mkati mwazachilengedwe zovutazi.
Mapulatifomu: Illumina NovaSeq ndi DNBSEQ-T7
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Metagenomic Sequencing-TGS
Metagenome ndi mndandanda wazinthu zamtundu wamagulu osakanikirana a zamoyo, monga chilengedwe ndi anthu. Lili ndi ma genomes omwe amalimidwa komanso osalimidwa. Kutsatizana kwa Metagenomic kumathandizira kusanthula kwamitundu yodabwitsayi yophatikizidwa mu zitsanzo zachilengedwe popereka zambiri kuposa mbiri ya taxonomic. Imaperekanso mawonekedwe ogwiritsira ntchito ma genomics poyang'ana majini osungidwa ndi maudindo awo pazochitika zachilengedwe. Ngakhale kuti mfuti zachikhalidwe zimayandikira ndi kutsatizana kwa Illumina zakhala zikugwiritsidwa ntchito kwambiri m'maphunziro a metagenomic, kubwera kwa Nanopore ndi PacBio kuwerengera kwanthawi yayitali kwasintha gawoli. Ukadaulo wa Nanopore ndi PacBio umakulitsa kusanthula kwa bioinformatic, makamaka msonkhano wa metagenome, kuwonetsetsa kuti misonkhano ipitirire. Malipoti akuwonetsa kuti Nanopore-based and PacBio-based metagenomics apanga bwino ma genome athunthu ndi otsekedwa a bakiteriya kuchokera ku ma microbiomes ovuta (Moss, EL, et al., Nature Biotech, 2020). Kuphatikiza mawerengedwe a Nanopore ndi mawerengedwe a Illumina kumapereka njira yowongolera zolakwika, kuchepetsa kulondola kwa Nanopore. Kuphatikizana kophatikizana kumeneku kumawonjezera mphamvu za nsanja iliyonse yotsatizana, kumapereka yankho lamphamvu kuti athe kuthana ndi malire omwe angakhalepo ndikupititsa patsogolo kulondola komanso kudalirika kwa kusanthula kwa metagenomic.
Platform: Nanopore PromethION 48, Illumia ndi PacBio Revio
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16S/18S/ITS Amplicon Sequencing-PacBio
Mitundu ya 16S ndi 18S rRNA, pamodzi ndi dera la Internal Transcribed Spacer (ITS), amagwira ntchito ngati zolembera zala zala zala chifukwa cha kuphatikiza kwawo madera otetezedwa komanso osinthika kwambiri, kuwapanga kukhala zida zamtengo wapatali zozindikiritsa zamoyo za prokaryotic ndi eukaryotic. Kukulitsa ndi kutsatizana kwa maderawa kumapereka njira yopanda kudzipatula yofufuzira kapangidwe ka tizilombo tating'onoting'ono komanso kusiyanasiyana kwachilengedwe. Ngakhale kutsatizana kwa Illumina nthawi zambiri kumayang'ana zigawo zazifupi ngati V3-V4 ya 16S ndi ITS1, zawonetsedwa kuti ndemanga zapamwamba za taxonomic zimatheka potsata kutalika kwa 16S, 18S, ndi ITS. Njira yonseyi imapangitsa kuti chiwerengero cha anthu chikhale chodziwika bwino, zomwe zimapangitsa kuti anthu azitha kuzindikira zamoyo. PacBio's Single-Molecule Real-Time (SMRT) yotsatirira nsanja imawonekera popereka zowerengera zazitali zolondola (HiFi) zomwe zimaphimba ma amplicons aatali, kupikisana ndi kulondola kwa Illumina. Kutha kumeneku kumalola ofufuza kuti apeze mwayi wosayerekezeka - mawonekedwe owoneka bwino a mawonekedwe amtundu. Kufalikira kokulirapo kumakweza kwambiri chigamulo m'zambiri zamitundu, makamaka m'magulu a bakiteriya kapena mafangasi, zomwe zimathandizira kumvetsetsa mozama za zovuta zamitundumitundu.
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16S/18S/ITS Amplicon Sequencing-NGS
Kutsatizana kwa Amplicon ndi ukadaulo wa Illumina, makamaka kutsata zolembera zamtundu wa 16S, 18S, ndi ITS, ndi njira yamphamvu yovumbulutsira kuchuluka kwa phylogeny, taxonomy, ndi mitundu yamitundu m'magulu ang'onoang'ono. Njira iyi imaphatikizapo kutsata zigawo zosasinthika za zolembera zanyumba. Poyambirira adawonetsedwa ngati chala chala chala ndiMavuto et almu 1977, njira iyi yasintha mbiri ya microbiome popangitsa kusanthula kopanda kudzipatula. Kupyolera mu kutsatizana kwa 16S (mabakiteriya), 18S (bowa), ndi Internal Transcribed Spacer (ITS, bowa), ofufuza amatha kuzindikira osati mitundu yambirimbiri komanso yosowa komanso yosadziwika. Chomwe chimatengedwa ngati chida chofunikira kwambiri, kutsatizana kwa amplicon kwathandizira kuzindikira mitundu yosiyanasiyana ya tizilombo tosiyanasiyana m'malo osiyanasiyana, kuphatikiza pakamwa pa munthu, matumbo, chopondapo, ndi kupitirira apo.
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Mabakiteriya ndi fungal Yense ya Genome Yotsatizana
Ntchito zotsatizananso za mabakiteriya ndi mafangasi amtundu wonse ndizofunikira kwambiri pakupititsa patsogolo ma genomics a microbial genomics pothandizira kukwaniritsidwa ndi kufananiza ma genome a microbial. Izi zimathandizira uinjiniya wa fermentation, kukhathamiritsa kwa njira zamafakitale, komanso kufufuza njira zachiwiri za metabolism. Kuphatikiza apo, kutsatizananso kwa mafangasi ndi mabakiteriya ndikofunikira kuti timvetsetse kusintha kwa chilengedwe, kukhathamiritsa zovuta, komanso kuwulula zakusintha kwa majini, zomwe zimakhudza kwambiri zamankhwala, ulimi, ndi sayansi ya chilengedwe.
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Kutsata kwa Prokaryotic RNA
Kutsatizana kwa RNA kumapatsa mphamvu kulongosola kwatsatanetsatane kwa zolembedwa zonse za RNA m'maselo pansi pamikhalidwe inayake. Ukadaulo wotsogolawu umagwira ntchito ngati chida champhamvu, kuwulula mbiri yakale ya jini, kapangidwe ka majini, ndi njira zama cell zomwe zimagwirizanitsidwa ndi mitundu yosiyanasiyana yachilengedwe. Kutengera kwambiri pakufufuza kofunikira, kuwunika kwachipatala, komanso chitukuko chamankhwala, kutsatizana kwa RNA kumapereka chidziwitso pazovuta zama cellular dynamics komanso kuwongolera majini. Kukonzekera kwathu kwa prokaryotic RNA kumapangidwira zolemba za prokaryotic, zomwe zikuphatikiza kuchepa kwa rRNA ndikukonzekera laibulale yowongolera.
Platform: Illumina NovaSeq
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Kutsata kwa Metatranscriptome
Tekinoloje yotsatizana ya Illumina, BMKGENE's metaranscriptome sequencing service ikuwulula mawonekedwe amtundu wamitundu yosiyanasiyana ya tizilombo tating'onoting'ono tosiyanasiyana, totengera ma eukaryotes mpaka ma prokaryotes ndi ma virus, m'malo achilengedwe monga dothi, madzi, nyanja, chimbudzi, ndi matumbo. Ntchito yathu yokwanira imapatsa mphamvu ofufuza kuti afufuze mbiri yamitundu yonse yamagulu ocheperako. Kupitilira kusanthula kwa taxonomic, ntchito yathu yotsatizana ya metatranscriptome imathandizira kufufuza pakulemeretsa kwa magwiridwe antchito, kuwunikira ma jini owonetsedwa mosiyanasiyana ndi maudindo awo. Dziwani zambiri zazachilengedwe mukamayang'ana mawonekedwe ovuta a ma gene, mitundu yosiyanasiyana ya taxonomic, komanso magwiridwe antchito mkati mwamitundu yosiyanasiyana yazachilengedwe.
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Ndi Novo Fungal Genome Assembly
BMKGENE imapereka mayankho osunthika a ma genomes a mafangasi, yothandiza pazosowa zosiyanasiyana za kafukufuku komanso kukwanira kwa ma genome. Kugwiritsa ntchito kusanja kwa Illumina kwakanthawi kochepa kumalola kupanga ma genome. Mawerengedwe afupikitsa komanso owerengera nthawi yayitali pogwiritsa ntchito Nanopore kapena Pacbio amaphatikizidwa kuti apange genome yoyengedwa bwino kwambiri yokhala ndi ma contig ataliatali. Kuphatikiza apo, kuphatikiza kutsatizana kwa Hi-C kumapititsa patsogolo luso, ndikupangitsa kuti munthu athe kupeza mtundu wathunthu wamtundu wa chromosome.
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Ndi Novo Bacterial Genome Assembly
Timapereka msonkhano wathunthu wa mabakiteriya a genome, kutsimikizira mipata 0. Izi ndizotheka pophatikiza matekinoloje otsatizana owerengera nthawi yayitali, monga Nanopore ndi PacBio pakuphatikiza ndi kuwerengera mwachidule ndi Illumina pakutsimikizira msonkhano ndikuwongolera zolakwika za kuwerenga kwa ONT. Ntchito yathu imapereka mayendedwe athunthu a bioinformatic kuchokera pamisonkhano, ndemanga zogwira ntchito, ndi kusanthula kwapamwamba kwa bioinformatics, kukwaniritsa zolinga za kafukufuku. Ntchitoyi imathandizira kupanga ma genomes olondola pamafukufuku osiyanasiyana amtundu ndi ma genomic. Kuphatikiza apo, imapanga maziko ogwiritsira ntchito monga kukhathamiritsa kwa zovuta, uinjiniya wa majini, ndi chitukuko chaukadaulo wa tizilombo tating'onoting'ono, kuwonetsetsa kuti deta yodalirika komanso yopanda malire ndiyofunikira pakupititsa patsogolo chidziwitso cha sayansi ndi luso lazopangapanga.
