-
BMKMANU S3000_Spatial Transcriptome
Spatial transcriptomics imatsogola pazatsopano zasayansi, kupatsa mphamvu ofufuza kuti afufuze movutikira momwe ma jini amafotokozera mkati mwa minofu ndikusunga malo awo. Pakati pa nsanja zosiyanasiyana, BMKGene yapanga BMKManu S3000 Spatial Transcriptome Chip, yomwe imadzitamandira ndi 3.5µm, yofikira pamagulu ang'onoang'ono, ndikupangitsa makonda amitundu yambiri. Chip cha S3000, chokhala ndi mawanga pafupifupi 4 miliyoni, chimagwiritsa ntchito tinthu tating'onoting'ono tomwe timakhala ndi mikanda yodzaza ndi zojambulidwa za barcode. Laibulale ya cDNA, yopangidwa ndi ma barcode a malo, imakonzedwa kuchokera ku chipangizo cha S3000 ndikutsatiridwa papulatifomu ya Illumina NovaSeq. Kuphatikiza kwa zitsanzo zokhala ndi barcode ndi UMIs zimatsimikizira kulondola ndi kutsimikizika kwa deta yopangidwa. Chip cha BMKManu S3000 ndi chosinthika kwambiri, chopereka zosintha zamitundu ingapo zomwe zimatha kusinthidwa bwino kumitundu yosiyanasiyana komanso tsatanetsatane watsatanetsatane. Kusinthasintha kumeneku kumayika chip ngati chisankho chabwino kwambiri pamaphunziro osiyanasiyana amtundu wapamalo, kuwonetsetsa kusanja kolondola kwa malo popanda phokoso lochepa. Kugwiritsa ntchito ukadaulo wogawa ma cell ndi BMKManu S3000 kumathandizira kugawa zolembera kumalire a maselo, zomwe zimapangitsa kusanthula komwe kuli ndi tanthauzo lachilengedwe. Kuphatikiza apo, kusintha kwabwino kwa S3000 kumabweretsa kuchuluka kwa majini ndi ma UMI omwe amapezeka pa selo iliyonse, zomwe zimapangitsa kusanthula kolondola kwambiri kwa mawonekedwe a malo osindikizira ndikuphatikizana kwa ma cell.
-
DNBSEQ malaibulale opangidwa kale
DNBSEQ, yopangidwa ndi MGI, ndiukadaulo waukadaulo wa NGS womwe wakwanitsa kutsitsa mtengo wotsatizana ndikuwonjezera zotuluka. Kukonzekera kwa malaibulale a DNBSEQ kumaphatikizapo kugawikana kwa DNA, kukonzekera kwa ssDNA, ndikukweza mozungulira kuti mupeze ma nanoballs a DNA (DNB). Izi zimayikidwa pamalo olimba ndikutsatiridwa ndi combinatorial Probe-Anchor Synthesis (cPAS). Ukadaulo wa DNBSEQ umaphatikiza zabwino zokhala ndi cholakwika chokulitsa chocheperako ndikugwiritsa ntchito zolakwika za kachulukidwe kakang'ono ndi ma nanoballs, zomwe zimapangitsa kutsatana ndi kutulutsa kwakukulu komanso kulondola.
Ntchito yathu yotsatirira laibulale yopangidwa kale imathandizira makasitomala kukonzekera malaibulale otsatizana a Illumina kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, malaibulale 10x, pakati pa ena), omwe amasinthidwa kukhala malaibulale a MGI m'ma laboratories athu kuti atsatidwe mu DNBSEQ-T7, kuchuluka kwa data pamitengo yotsika.
-
Hi-C yochokera ku Chromatin Interaction
Hi-C ndi njira yomwe idapangidwa kuti ijambule kasinthidwe ka genomic pophatikiza kufufuza kuyanjana kochokera kufupi ndi kutsata kwapamwamba. Njirayi imachokera ku chromatin crosslinking ndi formaldehyde, kutsatiridwa ndi kugaya ndi kubwezeretsanso m'njira yoti tizidutswa tating'ono tating'ono tating'ono timene timagwirizanitsa timapanga katundu wa ligation. Posanja zinthu za ligation izi, ndizotheka kuphunzira gulu la 3D la genome. Hi-C imathandiza kuphunzira kugawidwa kwa magawo a genome omwe ali odzaza pang'ono (A compartments, euchromatin) ndipo amatha kukhala olembedwa bwino, ndi zigawo zomwe zimakhala zolimba kwambiri (B compartments, Heterochromatin). Hi-C itha kugwiritsidwanso ntchito kuzindikiritsa Topologically Associated Domains (TADs), zigawo za genome zomwe zimapindika ndipo zimatha kukhala ndi mawonekedwe ofanana, komanso kuzindikira malupu a chromatin, zigawo za DNA zomwe zimalumikizidwa pamodzi ndi mapuloteni komanso omwe ali. nthawi zambiri amalemeretsedwa muzinthu zowongolera. Ntchito yotsatizana ya BMKGene ya Hi-C imapatsa mphamvu ofufuza kuti afufuze kukula kwa ma genomics, ndikutsegula njira zatsopano zomvetsetsa malamulo a genome ndi zotsatira zake paumoyo ndi matenda.
-
TGuide Smart Magnetic Plant RNA Kit
TGuide Smart Magnetic Plant RNA Kit
Yeretsani RNA yapamwamba kwambiri kuchokera kumagulu a zomera
-
TGuide Smart Blood/Cell/Tissue RNA Kit
TGuide Smart Blood/Cell/Tissue RNA Kit
Makina odzaza katiriji / mbale reagent kuti ayeretse zokolola zambiri, zoyera kwambiri, zamtundu wapamwamba, zopanda inhibitor zopanda RNA kuchokera ku minofu yanyama/selo/magazi athunthu.
-
TGuide Smart Magnetic Plant DNA Kit
TGuide Smart Magnetic Plant DNA Kit
Yeretsani DNA yapamwamba kwambiri kuchokera kumagulu osiyanasiyana a zomera
-
TGuide Smart Soil / Stool DNA Kit
TGuide Smart Soil / Stool DNA Kit
Imayeretsa DNA yopanda inhibitor kuti ikhale yoyera komanso yapamwamba kuchokera ku dothi ndi zitsanzo za ndowe
-
TGuide Smart DNA Purification Kit
Imabwezeretsanso DNA yapamwamba kwambiri kuchokera ku PCR kapena ma gels a agarose.
-
TGuide Smart Blood Genomic DNA Kit
TGuide Smart Blood Genomic DNA Kit
Makina odzaza katiriji / mbale reagent kuti ayeretse ma genomic DNA kuchokera kumagazi ndi malaya a buffy
-
TGuide Smart Magnetic Tissue DNA Kit
Makina odzaza katiriji / mbale reagent yochotsa ma genomic DNA kuchokera ku nyama
-
TGuide Smart Universal DNA Kit
Makina odzaza katiriji / mbale reagent kuti ayeretse ma genomic DNA kuchokera m'magazi, malo owuma amagazi, mabakiteriya, ma cell, malovu, swabs pakamwa, minofu yanyama, ndi zina zambiri.
-
TGuide S16 Nucleic Acid Extractor
TGuide S16 Nucleic Acid Extractor
Chida Chosavuta Kugwiritsa Ntchito Benchtop, 1-8 Kapena Zitsanzo 16 Nthawi Imodzi
Nambala ya Catalog / phukusi
Mphaka. ayi
ID
No. ya preps
OSE-S16-AM
1 seti
-
PacBio 2+3 Full-Length mRNA Solution
Ngakhale kutsatizana kwa mRNA kochokera ku NGS ndi chida chosunthika chowerengera ma jini, kudalira kwake pamawerengedwe afupikitsa kumalepheretsa kugwira ntchito kwake pakuwunika zovuta za transcriptomic. Kumbali ina, PacBio sequencing (Iso-Seq) imagwiritsa ntchito ukadaulo wowerengera nthawi yayitali, zomwe zimathandizira kutsatizana kwa zolembedwa zazitali za mRNA. Njirayi imathandizira kufufuza mwatsatanetsatane njira zina zophatikizira, kuphatikizika kwa ma gene, ndi poly-adenylation, ngakhale sichosankha choyambirira cha gene expression quantification. Kuphatikizika kwa 2 + 3 kumatchinga kusiyana pakati pa Illumina ndi PacBio podalira kuwerengeka kwa PacBio HiFi kuti izindikire mndandanda wathunthu wa ma isoforms olembedwa ndi ma NGS kutsatizana kuti athe kuwerengera ma isoform ofanana.
Mapulatifomu: PacBio Sequel II/ PacBio Revio ndi Illumina NovaSeq;
