mRNA-seq (NGS) yokhala ndi genome
RNA-seq ndi chida chokhazikika pa sayansi ya moyo ndi mbewu, kuthetsa kusiyana pakati pa ma genome ndi ma protein. Mphamvu yake yagona pakuzindikira zolembedwa zatsopano ndikuwunika momwe amafotokozera muyeso limodzi. Amagwiritsidwa ntchito kwambiri poyerekezera ndi maphunziro a transcriptomic, kuwunikira majini okhudzana ndi mikhalidwe yosiyanasiyana kapena ma phenotypes, monga kufanizira zosinthika ndi mitundu yakuthengo kapena kuwulula ma jini pansi pamikhalidwe inayake. BMKCloud mRNA(Reference) APP imaphatikizira kuchuluka kwa mawu, kusanthula kwa mawu osiyanitsa (DEG), ndi mawonekedwe amatsatizana amasanthula mupaipi ya bioinformatics ya mRNA-seq(NGS) ndikuphatikiza mphamvu zamapulogalamu ofanana, kuwonetsetsa kusavuta komanso kugwiritsa ntchito bwino. Ogwiritsa ntchito amatha kukweza deta yawo ya RNA-seq pamtambo, pomwe App imapereka yankho lathunthu, loyimitsa kusanthula kwa bioinformatic. Kuphatikiza apo, imayika patsogolo zomwe kasitomala amakumana nazo, ndikupereka magwiridwe antchito malinga ndi zosowa za ogwiritsa ntchito. Ogwiritsa ntchito amatha kukhazikitsa magawo ndikupereka ntchito ya mapaipi pawokha, kuyang'ana lipoti lolumikizana, kuwona deta / zojambula ndi migodi yathunthu ya data, monga: kusankha jini ya chandamale, kusanja kwa magwiridwe antchito, kujambula, ndi zina zambiri.
nsanja:Ilumina, MGI
Njira:RNA-Seq
Kamangidwe: Zambiri, zoyera.
Mtundu wa library:fr-unstranded, fr-firststrand kapena fr-secondstrand
Kuwerenga kutalika:150 bp
Mtundu wa fayilo:*.fastq, *.fq, *.fastq.gz kapena *.fq.gz. Dongosolo lidzaterophatikizani mafayilo a .fastq molingana ndi mayina awo,mwachitsanzo *_1.fastq yolumikizidwa ndi *._2.fastq.
Nambala ya zitsanzo:Palibe zoletsa pa nambalaza zitsanzo, koma nthawi yowunikira idzawonjezeka ngati chiwerengero chazitsanzo kukula.
Ndalama Zomwe Zaperekedwa:6G pa chitsanzo