Nanopore-based Full-length Transcriptome Sequencing
Nanopore sequencing imadzisiyanitsa ndi nsanja zina zotsatizana, chifukwa ma nucleotides amawerengedwa mwachindunji popanda kaphatikizidwe ka DNA ndipo amapanga kuwerengera nthawi yayitali pa makumi a kilobases. Izi zimathandizira kuwerengera molunjika kwa zolembedwa zazitali zonse ndikuthana ndi zovuta mu maphunziro a transcriptomic.
√ Kusakonda kotsatizana kocheperako
√ Kuwerenga kwautali kwa cDNA ku maphunziro a jini
√ Zambiri zomwe zimafunikira kuti mupeze zolemba zomwezo
√ Kuzindikiritsa ma isoform angapo pa jini
√ Kuchulukira kwamafotokozedwe pamlingo wa isoform
Pipeline Yodziwika ya Bioinformatics
Milandu Yaposachedwa Yopambana Ndi Biomarker Technologies
1. Kuwunika kofananira kwa zolembedwa zazitali zazitali zimawulula mphamvu zakukula kwa Gnetum luofuense.
Journal: Frontiers in Genetics
Lofalitsidwa: Marichi 2021
Mawu ofunika: MinION | Alternative splicing | APA | lncRNA | Zolemba zolembedwa mosiyanasiyana
Werengani Mawu Athunthu
2. Kusuntha kwapang'onopang'ono kwa ma RNA ang'onoang'ono kuchokera ku mphukira kupita ku mizu mu interspecific heterografts
Journal: Zomera Zachilengedwe
Lofalitsidwa: Jan. 2021
Mawu ofunika: Nanopore | Ilumina | kuzindikira kwakutali kwa mRNA yam'manja
3. Kusanthula kwautali wamtundu wa mizu ya katsitsumzukwa kumawonetsa momwe maselo amalekerera mchere chifukwa cha bowa wa arbuscular mycorrhizal.
Journal: Zomera Zachilengedwe ndi Zoyeserera
Lofalitsidwa: Jan. 2021
Mawu ofunika: MinION | Zolemba zosiyanasiyana | Zinthu zolembera | Alternative splicing
4. Kusintha kwa Makhalidwe Athupi ndi Kulemba Kwautali Wonse wa Rose (Rosa chinensis) Mizu ndi Masamba Pothana ndi Kupsinjika kwa Chilala
Journal: Zomera ndi Ma cell Physiology
Lofalitsidwa: Oct. 2020
Mawu ofunika: PromethION | Zolemba zosiyanasiyana | Kuneneratu kogwira ntchito | Ma isoform osiyanasiyana | TF ndi lncRNA
5. Kusanthula ndi kuyerekezera kwathunthu kwa PacBio ndi kutsata kwa RNA yochokera ku nanopore ya Arabidopsis transcriptome
Journal: Zomera Njira
Lofalitsidwa: Apr. 2020
Mawu ofunika: GridION | PromethION | PacBio Sequel | Illumina NovaSeq | Kulumikizana kwa genome | Chizindikiro cha zolemba | Alternative splicing | SSR | LNCRNA | Isoform quantification
Nkhani ndi Mfundo Zazikulu ikufuna kugawana milandu yaposachedwa kwambiri ndi Biomarker Technologies, kujambula zomwe zakwaniritsa zasayansi komanso njira zodziwika bwino zomwe zimagwiritsidwa ntchito pophunzira.
Nthawi yotumiza: Jan-08-2022