Illumina amalaibulale opangidwa kale
Mawonekedwe
●Mapulatifomu:Illumina NovaSeq 6000 ndi NovaSeq X Plus
●Masanjidwe:PE150 ndi PE250
●Kuwongolera kwabwino kwama library musanayambe kutsatizana
●Kutsata ma data QC ndi kutumiza:kubweretsa lipoti la QC ndi data yaiwisi mumtundu wa fastq mutatha demultiplexing ndikusefa Q30 yowerengedwa
Ubwino wa Utumiki
●Kusiyanasiyana kwa Sequencing Services:wogula angasankhe kutsatizana ndi kanjira, cell cell, kapena kuchuluka kwa deta yofunikira (kutsatizana kwa kanjira).
●Zochitika zambiri pa nsanja ya Illumina:ndi masauzande ambiri otsekedwa ndi mitundu yosiyanasiyana.
●Kutumiza kwa sequencing lipoti la QC:ndi ma metrics abwino, kulondola kwa data komanso magwiridwe antchito onse a projekiti yotsatizana.
●Njira yotsatirira anthu okhwima:ndi nthawi yochepa yozungulira.
●Kuwongolera Kwabwino Kwambiri: timakhazikitsa zofunikira za QC kuti zitsimikizire kuperekedwa kwa zotsatira zapamwamba nthawi zonse.
Zitsanzo Platform
| nsanja | Flow Cell | Njira yotsatirira | Chigawo | Zongoyerekeza |
| NovaSeq X | 10B (njira 8) | Mtengo wa PE150 | Msewu Waung'ono Partial Lane | 375Gb / njira |
| 25B (8 njira) | Mtengo wa PE150 | Msewu Waung'ono Partial Lane | 1000 Gb / Lane | |
| NovaSeq 6000 | Selo la SP Flow (njira ziwiri) | Chithunzi cha PE250 | Flow Cell Msewu Waung'ono Partial Lane | 325-400 M amawerenga / Lane |
| S4 Flow cell (njira 4) | Mtengo wa PE150 | Flow Cell Msewu Waung'ono Partial Lane | ~ 800 Gb / Njira |
Zitsanzo Zofunika
| Mtengo wa data (X) | Kukhazikika (qPCR/nM) | Voliyumu | |
| Kutsatizana Mwapang'ono
| X ≤ 10 Gb | ≥1 nM | ≥ 25 μl |
| 10 Gb <X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb <X ≤ 100 Gb | ≥3 nM | ≥ 25 μl | |
| X> 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Kutsata Njira | Pa Lane | ≥ 1.5 nM / dziwe la library | ≥ 25 μl / dziwe la library |
Kuphatikiza pa kukhazikika komanso kuchuluka kwake, pafunikanso patali yoyenera.
Zindikirani: Kutsatizana kwa malaibulale ocheperako kumafuna PhiX spike-in kuti mutsimikizire kuyimba koyambira.
Tikukulimbikitsani kuti mupereke malaibulale osankhidwa kale ngati zitsanzo. Ngati mukufuna BMKGENE kuchita kugawa laibulale, chonde onani
zofunikira laibulale yotsatizana pang'ono.
Kukula kwa Library (Mapu apamwamba)
Chiwopsezo chachikulu chikuyenera kukhala mkati mwa 300-450 bp.
Malaibulale ayenera kukhala pachimake chachikulu chimodzi, osayipitsidwa ndi adaputala komanso ma dimers oyambira.
Chonde tithandizeni ngati zitsanzo zanu sizikukwaniritsa zofunikira zoyambira.
Njira ya utumiki
Kuwongolera khalidwe la library
Kutsata
Kuwongolera khalidwe la deta
Kutumiza kwa polojekiti
Library QC lipoti
Lipoti laubwino wa laibulale imaperekedwa musanasande, kuwunika kuchuluka kwa laibulale, ndi kugawikana.
Kutsata lipoti la QC
Gulu 1. Ziwerengero za kusanja deta.
| Chitsanzo cha ID | BMKID | Yaiwisi amawerenga | Raw Data (bp) | Zowerenga zoyera (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Chithunzi 1. Kugawa kwabwino pamodzi ndi kuwerenga mu chitsanzo chilichonse
Chithunzi 2. Base content content
Chithunzi 3. Kugawa zowerengera zowerengera potsata deta
