Kusanthula kusanthula kosalekeza
Ubwino Wantchito
Takagi et al., Buku la mbewu, 2013
● Kulembetsa molondola: Kusakanikirana ndi anthu 30 mpaka 200 mpaka 200+ 200 kuti achepetse phokoso lakumbuyo; kulosera kosagwirizana ndi omalizidwa.
● Kusanthula kwathunthu: Mumpikisano Wosachedwa Gene, kuphatikiza nr, swissprot, pitani, Keg, Kog, Kog, etch, etc.
● Nthawi yotembenuka mwachangu: Gener gene komwe kumathamangira mkati mwa masiku 45 ogwira ntchito.
● Zokumana nazo kwambiri: BMK yathandizira m'mitundu ingapo, kuphimba mitundu yosiyanasiyana ngati mbewu, zinthu zopangidwa kum'madzi, nkhalango, zipatso, ndi zina.
Zolemba
Kuchuluka:
Kugawana ndi kubereka kwa makolo omwe ali ndi phenotypes.
EG F2 Progeny, BORCOROREST (BC), yobwezera ngongole ya inbred (Ril)
Kusakaniza dziwe
Makhalidwe oyenerera: 30 mpaka 50 payekha (osachepera 20) / zochuluka
Kwa kachulukidwe kambiri: yapamwamba 5% kwa anthu 10% omwe ali ndi zochitika zochulukirapo m'magulu onse (osachepera 30 + 30).
Kuzama Kuzama
Osachepera 20x / Kholo ndi 1x / ana payekha (mwachitsanzo, kwa ana osakanizira pa dziwe 30
Bioinform
● Kusintha konse kwa genome
● Kusintha kwa data
● SNP / SANDE
● Dera loyang'ana
● Gen
Zofunikira ndi Kutumiza
Zofunikira:
Nucleotides:
| GDNA SAMPT | Minofu yachiwiri |
| Kuzindikira: ≥30 ng / μl | Zomera: 1-2 g |
| Ndalama: μ2 μg (voling ≥15 μL) | Nyama: 0.5-1 G |
| Zoyera: Od260 / 280 = 1.6-2.5 | Magazi Onse: 1.5 ml |
Ntchito Yogwira Ntchito
Mapangidwe a kuyesa
Kutumiza kwachitsanzo
Kutulutsa kwa RNA
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Kusanthula kwa 25ssassiations pa mtunda wa euclidean (Ed) kuti azindikire dera. Mu chithunzi chotsatira
X-Axis: Nambala ya chromosome; Doko lirilonse limayimira mtengo wa enp. Mzere wakuda umagwirizana ndi mtengo woyenerera. Mtengo wapamwamba kwambiri umawonetsa mgwirizano waukulu pakati pa tsamba ndi phenotype. Mzere wofiyira wofiyira umayimira kumulanda.
2. Kusanthula kwa 2.Sassiation Kutengera Snow-Index
X-Axis: Nambala ya chromosome; Doko lililonse limayimira mtengo wa SNP-Index. Mzere wakuda umayimira mtengo woyenera. Chokulirapo mtengo wake ndiye, chofunikira kwambiri kuyanjana.
Nkhani ya BMK
Mkhalidwe Wazikulu Zochulukirapo Ones Fnl7.1 Kumata kwathanzi lakumapeto kochuluka komwe kumalumikizidwa ndi kutalika kwa khomo la zipatso mu nkhaka
Yofalitsa: Bzalani BOOTETECHNOST YECOY, 2020
Njira Zoyeserera:
Makolo (Jin5-508, Yn): Kusintha konse kwa genome kwa 34 × ndi 20 × 1.
Ma Ports a DNA (50-quanced ndi 50-fupikisi): Kukhazikitsanso kwa 61 × ndi 52 ×
Zotsatira zazikulu
Mu kafukufukuyu, anthu osokoneza bongo (F2 ndi F2: 3) adapangidwa podutsa nkhaka zazitali za khosi jin5-508 ndi yn yayifupi. Madziwe awiri a DNNA adapangidwa ndi anthu otalikirapo 50 ndi khosi lalifupi ndi makhosi pafupifupi 50. Makampani akuluakulu a QTL adazindikiridwa pa Chr07 ndi kuwunika kwa BSAA ndi mapu a qtl. Dera loyesedwa lidawunikidwanso pansi pa mapu abwino, majini a gene oyeserera, omwe amawululira majini ofunikira pakuwongolera khosi, CSFNL7.1. Kuphatikiza apo, polymorphism mu CSFNL7.1 Dera Lotsogolera linapezeka kuti likugwirizana ndi mawu ofananira. Kusanthulanso kwa phylogenetic inanena kuti FNL7.1 EMUS ndiyoyambira ku India.
 Mapu a Qtl-ku BSA akuwunika kuti azindikire kudera lokhala ndi nkhaka |  Mafayilo a LOD a nkhaka khosi-kutalika kwa QTL kuzindikirika pa Chr07 |
XU, X., et al. "Trait Wamtundu wambiri wowerengeka wa FNL7.1 Amakhala ndi mapuloteni okwanira ochuluka omwe amalumikizidwa ndi kutalika kwa khomo la zipatso mu nkhaka." Bzalani biotechnology Journation 18.7 (2020).
