BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Mawonekedwe
● Kusamvana: 5 µM
● Spot Diameter: 2.5 µM
● Chiwerengero cha malo: pafupifupi 2 Miliyoni
● 3 mitundu yokhoza kujambula: 6.8 mm * 6.8 mm, 11 mm * 11 mm kapena 15 mm * 20 mm
● Mkanda uliwonse wa barcode umadzazidwa ndi zoyambira zomwe zili ndi magawo 4:
poly(dT) mchira wa mRNA priming ndi cDNA synthesis
Unique Molecular Identifier (UMI) kuti akonze kukondera kokulitsa
Malo barcode
Kumangirira koyambira pang'ono kuwerenga 1 koyambira
● H & E ndi madontho a fulorosenti a zigawo
● Kutheka kugwiritsa ntchitoteknoloji yogawa ma cell: kuphatikizika kwa madontho a H&E, utoto wa fulorosenti, ndi kutsatizana kwa RNA kuti mudziwe malire a selo lililonse ndikugawa molondola mawonekedwe a jini ku selo lililonse.
Ubwino wa BMKMANU S1000
●Sub-cellular Resolution: Malo aliwonse olandirira amakhala ndi >2 miliyoni Madontho A Barcode okhala ndi mainchesi 2.5 µm ndi motalikirana 5 µm pakati pa malo, zomwe zimathandiza kusanthula kwa malo okhala ndi ma sub-cellular resolution (5 µm).
●Multi-level Resolution Analysis:Kusanthula kosinthika kwamitundu ingapo kuyambira 100 μm mpaka 5 μm kuti athetse mitundu yosiyanasiyana ya minofu pakuwongolera koyenera.
●Kutheka Kugwiritsa Ntchito “Three in one slide” Cell Segmentation Technology:Kuphatikiza madontho a fluorescence, madontho a H&E, ndi kutsatizana kwa RNA pa slide imodzi, algorithm yathu yowunikira "atatu-in-one" imathandizira kuzindikira malire a cell pama cell-based transcriptomics.
●Yogwirizana ndi Mapulatifomu Angapo Otsatira: Zonse za NGS ndi kutsatizana kowerenga kwa nthawi yayitali kulipo.
●Mapangidwe Osinthika a 1-8 Active Capture Area: Kukula kwa malo ogwidwa ndi osinthika, kotheka kugwiritsa ntchito mawonekedwe a 3 (6.8 mm * 6.8 mm., 11 mm * 11 mm ndi 15 mm * 20 mm)
●One-Stop Service: Imaphatikiza zochitika zonse ndi masitepe otengera luso, kuphatikiza cryo-sectioning, staining, kukhathamiritsa kwa minofu, barcoding ya malo, kukonzekera laibulale, kutsatizana, ndi bioinformatics.
●Comprehensive Bioinformatics and User-friendly Visualization of Results:Phukusi limaphatikizapo kusanthula kwa 29 ndi ziwerengero zapamwamba za 100+, kuphatikiza kugwiritsa ntchito mapulogalamu opangidwa m'nyumba kuti muwone ndikusintha mwamakonda kugawanika kwa ma cell ndi kusanja mawanga.
●Kusanthula Kwadongosolo Kwadongosolo ndi Kuwonera: kupezeka pazofunsira zosiyanasiyana
●Team yaukadaulo yaukadaulo: Wodziwa zambiri pamitundu yopitilira 250 ya minofu ndi mitundu 100+ kuphatikiza anthu, mbewa, zoyamwitsa, nsomba ndi zomera.
●Zosintha Zenizeni Pa Ntchito Yonse: ndi ulamuliro wonse wa kupita patsogolo kwa kuyesera.
●Kusanthula Kwapang'onopang'ono Kophatikizana ndi Sequencing ya single-cell mRNA
Mafotokozedwe a Utumiki
|
Chitsanzo Zofunikira
| Library |
Njira yotsatirira
| Deta yovomerezeka | Kuwongolera Kwabwino |
| OCT-ophatikizidwa cryo zitsanzo, 3 midadada pa chitsanzo | S1000 cDNA library | Illumina PE150 (mapulatifomu ena alipo) | 100K PE imawerengedwa pa 100M (60-150 Gb) | RIN> 7 |
Kuti mumve zambiri zachitsogozo chokonzekera zitsanzo ndi kayendetsedwe ka ntchito, chonde omasuka kulankhula ndi aBMKGENE katswiri
Kuyenda kwa Ntchito Yantchito
Mu gawo lokonzekera lachitsanzo, kuyesa koyambirira kwa RNA kochulukira kumachitika kuti zitsimikizire kuti RNA yapamwamba kwambiri ingapezeke. Mu gawo la kukhathamiritsa kwa minofu zigawozo zimadetsedwa ndikuwonetseredwa komanso momwe permeabilization yotulutsa mRNA kuchokera ku minofu imakonzedwa. Protocol yokhathamiritsa imagwiritsidwa ntchito pomanga laibulale, ndikutsatiridwa ndi kutsata ndi kusanthula deta.
Kuyenda kwathunthu kwautumiki kumaphatikizapo zosintha zenizeni zenizeni ndi kutsimikizika kwamakasitomala kuti mukhalebe ndi mayankho omvera, kuwonetsetsa kuti polojekiti ichitike bwino.
Deta yopangidwa ndi BMKMANU S1000 imawunikidwa pogwiritsa ntchito pulogalamu ya "BSTMatrix", yomwe imapangidwa paokha ndi BMKGENE, ikupanga Gene Expression Matrix. Kuchokera pamenepo, lipoti lokhazikika limapangidwa lomwe limaphatikizapo kuwongolera khalidwe la deta, kusanthula kwachitsanzo chamkati ndi kusanthula magulu.
● Kuwongolera Ubwino wa Data:
Kutulutsa kwa data ndi kugawa kwamagulu abwino
Kuzindikira kwa majini pa malo
Kuphimba minofu
● Kusanthula kwachitsanzo chamkati:
Kulemera kwa gene
Spot clustering, kuphatikizapo kuchepetsedwa kwa dimension
Kusanthula kwamaganizidwe osiyanasiyana pakati pa magulu: kuzindikiritsa jini zolembera
Kufotokozera mogwira ntchito komanso kukulitsa ma jini olembera
● Kusanthula magulu:
Kuphatikizanso mawanga kuchokera ku zitsanzo zonse ziwiri (monga matenda ndi kuwongolera) ndikuphatikizanso
Kuzindikiritsa jini zolembera pagulu lililonse
Kufotokozera mogwira ntchito komanso kukulitsa ma jini olembera
Kusiyana Kufotokozera kwa gulu lomwelo pakati pa magulu
Kuphatikiza apo, BMKGENE yapanga "BSTViewer" ndi chida chosavuta kugwiritsa ntchito chomwe chimathandiza wogwiritsa ntchito kuwona m'maganizo amtundu wa jini ndikuphatikizana pamalingaliro osiyanasiyana.
BMKGene idapanga pulogalamu yowonera ogwiritsa ntchito mwaubwenzi
BSTViewer masango akuphatikizana pamasankho amitundu yambiri
BSTCellViewer: kugawanika kwa ma cell ndi manja
Kusanthula kwachitsanzo chamkati
Spot clustering:
Chizindikiritso cha majini a zikho ndikugawa malo:
Kusanthula kwamagulu
Kuphatikiza kwa data kuchokera m'magulu onse ndi gulu lophatikizanso:
Ma genes a magulu atsopano:
Onani kupita patsogolo komwe kwayendetsedwa ndi BMKGene's spatial transcriptomics services ndi ukadaulo wa BMKManu S1000 m'bukuli:
Nyimbo, X. et al. (2023) 'Spatial transcriptomics imawulula ma cell a chlorenchyma opangidwa ndi kuwala omwe amathandizira kulimbikitsa kusinthika kwa mphukira mu tomato callus', (2023)Zokambirana za National Academy of Sciences ku United States of America, 120(38), p. e2310163120. doi: 10.1073/pnas.2310163120
Inu, Y. et al. (2023) 'Kuyerekeza kwadongosolo kwa njira zotsatirira zotengera malo',bioRxiv, p. 2023.12.03.569744. doi: 10.1101/2023.12.03.569744.
