10X Funomics Visium Spain
CHENJEZO
Mawonekedwe
● Kusintha: 100 μm
● M'mawu apawiri: 55 μm
● Mapazi: 4992
● Dera lojambulidwa: 6.5 x 6.5 mm
● Malo osungirako aliyense amadzaza ndi mapulogalamu anayi:
- poly (dt) mchira wa MRNA yoyambira ndi CDna Synthesis
- Chidziwitso chapadera cha matope (UMI) kuti mukonze tsankho
- Barcode ya spatial
- Kuphatikizira kwa gawo limodzi lowerengera 1
● h & e kukhazikika pamagawo
Ubwino
●Ntchito yosiya: Amaphatikizana ndi luso lililonse komanso maluso okhudzana ndi luso, kuphatikizapo chidani, chokhazikika, kukhathamiritsa, kupindika kwa bioting, zokonzekera ndi bioinformatickatics.
● Gulu laukadaulo kwambiri: Ndi zokumana nazo zopitilira 250 minofu ndi mitundu 100+ kuphatikiza mitundu 100 kuphatikizapo anthu, mbewa, nsomba, nsomba ndi zomera.
●Kusintha kwenikweni pa ntchito yonse: Ndikuwongolera kupita patsogolo.
●Kukhazikika bioinfoctictics:Phukusi limaphatikizaponso kuwerenga 29 ziwerengero zapamwamba kwambiri.
●Kusanthula kwa data ndi zowunikira: Kupezeka ndi zopempha zosiyanasiyana.
●Kusanthula kwa kusankha kosankhana ndi mrna
Kulembana
| Zofunikira Zitsanzo | Nkhokwe ya mabuku | Njira Yolowetsa | Zambiri Zotsimikiziridwa | Kuwongolera kwapadera |
| Oct-Comded Steed Sporm (Weamental Weameter: pafupifupi. 6x6x6 mm³) 2 mabatani awiri pa chitsanzo | 10x Visium Cdna Library | Audina Pe150 | 50k pe imawerenga pamalopo (60GB) | Rin> 7 |
Kuti mumve zambiri pa zitsanzo zokonzekera ndi ntchito yogwira ntchito, chonde khalani omasuka kuyankhula ndi aKatswiri wa BMKGEne
Ntchito Yogwira Ntchito
Mu zitsanzo pokonzekera gawo, kuyesa kwa RNA Kuchotsa Kuchotsa kumachitika kuti awonetsetse kuti RNA yapamwamba kwambiri itha kupezeka. Mu Kutsanzira kwa minofu ya minofu kumangidwa ndi kuwoneka ndi kuwona bwino komanso momwe mungagwiritsire ntchito kwa MRNA zimakonzedwa. Pulogalamu yotsimikizika ikugwiritsidwa ntchito panthawi yomanga laibulale, kutsatiridwa ndi kupendekera komanso kusanthula kwa deta.
Ntchito yathunthu imaphatikizapo zosintha zenizeni komanso zotsimikizika za kasitomala kuti musunge chiuno choyankha, onetsetsani kuti kuphedwa kosavuta.
Mulinso kuwunika:
Kuwongolera kwa data:
o Kutulutsa kwa deta ndi kugawa kwabwino
o jeat penti pa malo
o minofu yophimba
Kusanthula kwamkati:
o In Innessness
o Lowetsani masanjidwe, kuphatikizapo kuchepa kwakanthawi
o Kusanthula kwa Maganizo pakati pa masango: Kuzindikiritsa majini a Marker
o Kugwiritsa ntchito kugwirira ntchito ndi zopindulitsa kwa majini a Marker
Kusanthula kwa magulu
o Kuphatikizanso malo kuchokera ku zitsanzo (mwachitsanzo. matenda ndi kuwongolera) ndikukonzanso
o chizindikiritso cha majini a Marker pa tsango lililonse
o Kugwiritsa ntchito kugwirira ntchito ndi zopindulitsa kwa majini a Marker
o Kusiyanitsa kosiyana ndi magulu omwewo pakati pamagulu
Kusanthula kwamkati
Malo omangika
Chizindikiro cha Marker chimadziwika ndi kugawa kwa shiti
Kusanthula kwa magulu
Kuphatikizidwa kwa data kuchokera m'magulu onse awiri ndikukonzanso
Zithunzi za Marker Zatsopano Zatsopano
Funsani Zinthu Zomwe Zimaphatikizidwa ndi BMKGEN
Chen, D. et al. (2023) 'MTLL1, yemwe ali ndi vuto la Drosophraila wa Ambeli a AndMolian Antshes, amakhudzidwa ndi zotsutsana ndi zomwe wavala zovala za oncogenic mu ntchentche',Zochitika za National Academy of Sayansi ya United States of America, 120 (30), p. E2303462120. Doi: /10.1073/pnas.2303422120
Chen, y. et al. (2023) 'Chitsulo chimathandizira kuwerengera kwapamwamba kwa ma transriptoract.Zachidule ku Bioinfoctic, 24 (2), mas. 1-10. Doi: 10.1093 / Bib / Bbad068.
Liu, C. et al. (2022) 'Zithunzi za Spachiraltural Atsongenesis pakukula kwa maluwa a orchid',Ma acid acids acids, 50 (17), mas. 9724-9737. Doi: 10,1093 / Pour / Gkac773.
Wang, J. et al. .Mabungwe apadziko lonse lapansi a sayansi, 19 (8), mas. 2515-2530. Doi: 10.7150 / IJbb.83510.
