TGuide S16 Tango Waikawa Nucleic
Whakataki hua
Ka taea e TGuide S16 Nucleic Acid Extractor te tukatuka ki te 16 tauira i te wa kotahi e nga rakau autō hei here me te whakawhiti i nga pirepire autō, te pereti 96-poka, te kariri reagent tauira kotahi ranei me te heru pito autō. Ma te whakamahi i te reagent tangohanga waikawa karihi e rite ana, ka taea e tenei hua te purei aunoa i te DNA/RNA mai i nga momo tauira kararehe, tipu, huakita, harore, me etahi atu, me te tango i te DNA mai i te plasmid, te gel agarose me te hua PCR.
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Ngā āhuatanga
Super ngawari ki te whakamahi
Karekau he mahi paipa atu. Ka taea te whakatika te rōrahi elution ki te hiahiatia.
Ko te whakaurunga me te mahi he iti noa te whakangungu. Ki nga papatono kua oti te whakauru, wetewete i te kariri, tohua te kawa ka whakahaere i to whakamatautau. Kua reri nga kaupapa ki te whakamahi, ka taea hoki te whakarite.
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Aratau here hou
Ka mau te rakau autō i te aratau here mutunga kaha.
Ka herea nga pirepire aukume ki te raro o te rakau, e whakarite ana ka taea tonu e te eluent te kapi katoa nga pirepire autō ahakoa he iti te rōrahi eluent. Ko te hoahoa ka whakanui i te hua o te waikawa nucleic.
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Te hoahoa tino pai o te tank whakawera
He tīwae kua whakauruhia ki waenga i te pūreirei lysis me te tīwae eluent, he pai te aukati i te eluent mai i te whakaeto na te whakamahana o te pou lysis. Na reira, he tika ake te kaha whakaora o te otinga waikawa karihi.
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Te mana whakakino me te whakamarumaru a te kaiwhakahaere
Ka taea te whakanoho ki runga i te tauera ma, ki roto ranei i te papa matū.
Kei te rere aunoa te taputapu me te kati.
E rua nga tikanga e whakamahia ana e te punaha whakahaere poke: ko te punaha whakahaere whakamaarama-a-roto me te punaha whakamaarama ultraviolet, kia kore ai e paopao i waenga i nga pou me nga tauira.
Ko nga reagents kua oti te utaina me nga taonga pau kua rite.
Ka whakakorehia te whakahaere matū ki te tino nui hei whakarite i te haumaru o te mahi.
Hua Whakaaturanga
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Hua whakamātautau: te whakamahi i te TGuide S16 me te reagent TGuide Smart Universal DNA Kit (4993704)
ki te tango aunoa i te DNA genomic o nga tauira maha tae atu ki nga pūtau, te kiko, te toto, te wahi toto maroke (DBS), Lactobacillus, E.coli, huware, me te swab, me etahi atu, te whakatutuki i te hua pai me te tino ma.
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Ko te OD260 / OD280 a tawhio noa 1.7 ~ 1.9, me
OD260/OD230＞1.6.
Rōrahi elution:100 μl
Te kukū o te kiaro: 1.5%
Te uta i te rōrahi: 1 μl
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Te whakamahi i te TGuide S16 me te reagent TGuide Smart Viral DNA/RNA Kit (4993702)
ki te tango aunoa i te DNA / RNA mai i nga puna maha tae atu ki te toto katoa, te kaukau me te plasma i tutuki te hua pai me te tino ma. -
Ko te OD260/OD280 huri noa i te 1.7~1.9, me te OD260/OD230 ＞ 1.6.
He kamupene me te kamupene B: Nga waitohu rongonui.
ND: Te mate Newcastle, H5: Rewharewha Avian, CP: Parvovirus Canine, ASF: Te mate kirikaa o Awherika
Ko te tairongo iti ka eke ki te 100 kape/ml.
Te hua whakamatautau: te whakamahi i te TGuide S16 me te reagent TGuide Smart Blood Genomic DNA Kit (4993703) ki te tango aunoa i te DNA genomic o te toto tio kua tutuki te hua pai me te tino parakore, he rite ki te hua tangohanga me te ma o nga hua whakataetae rongonui.
Ko te OD260/OD280 huri noa i te 1.7~1.9, me te OD260/OD230 ＞ 1.7.
Te puna tauira: E toru nga tauira toto katoa o te tangata. E toru nga tauira he whakamatautau mo nga kete takitahi.
Ko nga tauira mo ia kete mai i te puna tauira kotahi.
Turanga tauira: Toto tio
Rōrahi tāuru:200 μl
Rōrahi elution:100 μl
Te kukū o te kiaro: 1.5%
Te uta i te rōrahi: 1 μl
M: Marker III, TIANGEN
He kamupene me te kamupene B: Nga waitohu rongonui
2000 bp wāhanga agarose gel DNA purenga me te whakaora
Te kukū o te aporo: 1.5%(TBE)
Te uta i te rōrahi: 8 μl
Tohu: D2000, TIANGEN
Ko S16-01, S16-02, me S16-03 te tohu mo te purenga tauira whakarara me te whakamatautau whakaora ma te whakamahi i te 3 TGuide S16 Nucleic Acid Extractors me TGuide Smart DNA Purification Kit (4993550) i te wa ano.
He Kamupene: te kete tangohanga pou-a-ringa-a-ringa o tetahi waitohu rongonui
I whakamahia a Agilent 2100 mo te rapunga pono o te RNA.
Tauira puna: CD-1 kiore
Te mana tauira: toto katoa hou
Rahi tauira: 200 μl
Ko te hua o te whakamatautau: ko te tapeke RNA i tangohia mai i te toto e TGuide S16 me te TGuide Smart Blood/Cell/Tissue RNA Kit (4993551) he pai te tuku me te tino parakore, a ka taea e ia te whakatutuki i nga hiahia o te whakararo o te raupapatanga teitei.
Te uta i te rōrahi: 1 μl. Ko te ate i diluted 5 wa ka utaina. Te kukū o te kara kiarosa: 1%. Electrophoresis mo te 20 min i te 6 V/cm;
M: TIANGEN Tohu III
1-4: ngakau 5-8: ate 9-12: pūkahukahu 13-16: whatukuhu
Ko nga tauira tuatahi e rua o ia kiko i tangohia e te kete tangohanga i runga i te pou-a-mio, a ko nga mea whakamutunga e rua i tangohia aunoatia e TGuide S16.
Tauira puna: CD-1 kiore
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Hua whakamātautau: ko te DNA genomic kiko kararehe i tangohia aunoatia e TGuide S16 me te reagent TGuide Smart Tissue Tissue DNA Kit (4993547) he nui te tuku me te parakore, he rite ki te DNA i tangohia e te kawa tangohanga i runga i te pou hurihuri. He rite tonu te ma, me OD260/OD280 a tawhio noa 1.7~1.9 me OD260/OD230> 1.7. Hei whakamutunga, ka taea te whakamahi a TGuide S16 ki te tango i te DNA ira tangata mai i nga kopa kararehe hei whakakapi i te otinga tangohanga i runga i te pou-mio.
Katoa te tangohanga RNA mai i nga tauira tipu rereke
Rahi tauira: 100 mg
Tauira i mua-maimoatanga: homogenizer pāmahana iti
Te kutanga o te aporo: 1%(TAE)
Te uta i te rōrahi: 1 μl
M: Tohu III, TIANGEN
1-4: kākano kānga 5-8: rau kānga 9-12: pakiaka kāpeti 13-16: soybeans
Ko nga tauira tuatahi e rua i tangohia e te kete tangohanga pou-mio, ko nga tauira whakamutunga e rua i tangohia e TGuide S16.
Te tangohanga DNA genomic o nga pakiaka witi
Rahi tauira: 100 mg
Tauira i mua-maimoatanga: huri ki te hauota wai ranei kiko huri homogenizer
Te kutanga o te aporo: 1%(TAE)
Te uta i te rōrahi: 2 μl
Tohu: D15000, TIANGEN
He Kamupene: he tohu rongonui
Te hua whakamatautau: te whakamahi i te TGuide S16 me te reagent TGuide Smart Magnetic Plant DNA Kit (4993548) ki te tango aunoa i te DNA genomic o nga pakiaka witi kua tutuki te hua pai me te tino parakore, e rite ana ki te hua tangohanga me te ma o te whakataetae rongonui. I tua atu, ko te pono o te DNA genomic he pai ake i tera o nga hua whakataetae o etahi atu kamupene. I roto i tenei whakamatautau, tata ki te 15 μg o te waikawa karihi i tangohia mai i te 100 mg pakiaka witi, me te OD260/OD280 huri noa i te 1.8~1.9, me te OD260/OD230＞2.0.
