TGuide Ataahua Ataahua Tipu RNA Kit
Nama Putumōhio / tākainga
| ngeru. kahore | ID | Nama o preps |
| 4993552(karaata) | DP662-DE | 48 |
He aratohu whiriwhiri reagent mo TGuide S16
| Waikawa Nuclei | Momo Tauira | Ingoa Hua | Rahi kete (whakarite) Kaata/ pereti | ngeru No. Kaata/pereti |
| DNA | Tipa Kararehe | TGuide Smart Tissue Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Te tipu me te kakano | TGuide Ataahua Ataahua Tipu DNA Kit | 48 | 4993548 |
| DNA | Te oneone/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Hua kiri me PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Toto Katoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Pūtau/Swab/Wangi maroke, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Toto/Pūtau/Tipa | TGuide Smart Blood/Cutau/Tipa RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Te tipu me te kakano | TGuide Ataahua Ataahua Tipu RNA Kit | 48 | 4993552 |
| DNA/RNA | Wai Kore Kore Putau (Serum, etc) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Whakataki hua
Purihia te RNA katoa o te hua nui, te parakore, te kounga teitei mai i te toto / pūtau / kiko.
Karekau he reagents paitini penei i te phenol/chloroform kei roto
Ko te RNA kua whiwhi he tino parakore, ka taea te whakamahi tika mo te rapunga maramara, te raupapatanga teitei me etahi atu whakamatautau.
Ngā āhuatanga
Super ngawari ki te whakamahi
Karekau he mahi paipa atu.
Ka taea te whakatika te rōrahi elution ki te hiahiatia.
Ko te whakaurunga me te mahi he iti noa te whakangungu. Ki nga papatono kua oti te whakauru, wetewete i te kariri, tohua te kawa ka whakahaere i to whakamatautau. Kua reri nga kaupapa ki te whakamahi, ka taea hoki te whakarite.
Ko nga reagents kua oti te utaina me nga taonga pau kua rite.
Ka whakakorehia te whakahaere matū ki te tino nui hei whakarite i te haumaru o te mahi.
Hua Whakaaturanga
Te tangohanga RNA katoa o te pakiaka witi
Rahi tauira: 100 mg
Tauira pretreatment: huri ki te hauota wai ranei iti pāmahana maimoatanga homogenizer kiko
Te kukū o te aporo: 2%(TAE)
Te uta i te rōrahi: 2 μl
Tohu: Tohu IV, TIANGEN
Te hua whakamatautau: te whakamahi i te TGuide S16 i tutuki me te reagent 4993552 ki te tango aunoa i te tapeke RNA o nga pakiaka witi he pai te tuku me te tino ma. I tenei whakamatautau, tata ki te 20 μg o te waikawa karihi i tangohia mai i te 100 mg pakiaka witi, me te OD260/OD280 huri noa i te 2.0~2.1, me te OD260/OD230＞2.0.
Katoa te tangohanga RNA mai i nga tauira tipu rereke
Rahi tauira: 100 mg
Tauira pretreatment: homogenizer iti-mahana
Te kutanga o te aporo: 1% (TAE)
Te uta i te rōrahi: 1 μl
M: Tohu III, TIANGEN
1-4: kākano kānga 5-8: rau kānga 9-12: pakiaka kāpeti 13-16: soybeans
Ko nga tauira tuatahi e rua i tangohia e te kete tangohanga pou-mio, ko nga tauira whakamutunga e rua i tangohia e TGuide S16.
Hua whakamātautau: ko te hua tangohanga o te tipu RNA na TGuide S16 me te reagent 4993552 he rite, he pai ake ranei i tera e te kete tangohanga pou-mio. He rite tonu te ma me te tapatahi. No reira, ka taea te whakamahi a TGuide S16 ki te tango i te RNA katoa o te tipu hei whakakapi i te otinga tangohanga i runga i te pou-mio.
