Tguige Smart toto / pūtau / kete rota
Tau Katoa / Pakihi
| Ngeru. kāo | ID | Tuhinga o mua |
| 4995043 (pereti) | Dp661-e | 96 |
| 4993551 (kaata) | Dp661-de | 48 |
He Aratohu Whiriwhiringa Tagent mo TGGior S16
| Waikawa karihi | Momo tauira | Ingoa Hua | Te rahi o te rahi (Preps) Kaata / pereti | Ngeru kaore. Cartridge / pereti |
| Tī rapunga | Te kiko kararehe | Tguide Smart CNA Kit | 48/96 | 4993547/4995038 |
| Tī rapunga | Te tipu me te purapura | Tguide Smart CNA Kit | 48 | 4993548 |
| Tī rapunga | Oneone / tuohu | Tguide Smart oneone / Stool Kit | 48 | 4993549 |
| Tī rapunga | Nga hua gel & PCR | Tguide Smart DNA pure | 48 | 4993550 |
| Tī rapunga | Toto katoa | Tguige Smart GNA Kit | 48/96 | 4993703/4995206 |
| Tī rapunga | Cell Cell / Swab / maroke, me etahi atu | Tguide Smart Universal DNA kete | 48/96 | 4993704/4995040 |
| Rna | Toto / pūtau / kiko | Tguige Smart toto / pūtau / kete rota | 48/96 | 4993551/4995043 |
| Rna | Te tipu me te purapura | Tguide Smart RNA Kit | 48 | 4993552 |
| DNA / RNA | Nga rerenga koreutu (serum, me etahi atu) | Tguige Smart DNA / RNA Kit | 48/96 | 4993702/4995207 |
Kupu whakataki
Hangaia te hua nui, te tino ma, te nui o te katoa o te rna nui mai i te toto / pūtau / kiko.
Kaore he reagents paitini penei i te kaupapa / chloroform kei roto
I whiwhi te rna i te tino ma te whakamahi me te whakamahi tika mo te kitenga maramara, te huringa nui-whakauru me etahi atu whakamatautau.
Ngakau
He ngawari te whakamahi
Kaore he mahi whakaipoipo.
Ka taea te whakatika i te rōrahi whakatikatika.
Ko te whakaurunga me te mahi mahi he iti noa te whakangungu. Ki nga kaupapa kua whakauruhia, wetekina te kaata, tohua te kawa me te whakahaere i to whakamatautau. Kua rite te whakamahi i nga hotaka, me te whakamahi ano hoki.
I whakaritea e nga reagents me te whai i nga kaihoko whakaheke.
Ko te whakahaere matū ka whakakorehia ki te whānuitanga nui ki te whakarite i te haumaru whakahaere.
Ko te hua demo
I whakamahia te Adilent 2100 mo te kitenga tika.
He tauira tauira: Nga kiore CD-1
Tauira Tauira: Nga toto katoa
Rahi tauira: 200 μl
Ko te hua o te whakamatautau: Ko te katoa o te moni a Tguide S16 kua whakauruhia ki te reagent 4993551 he pai te whakaputa me te tino ma, ka taea te whakatutuki i nga hiahia o raro
Tuhinga o mua.
He tauira tauira: Nga kiore CD-1
Te Hoko Rorohiko: 1 μL. Ko te ate i te 5 nga wa ka utaina.
Te kukū a Agarose Gel: 1%. Electrophoresis mo te 20 min i te 6 V / cm;
M: Tiangen tohu III 1-4: Te ngakau 5-8:
ate 9-12: Lung 13-16: whatukuhu
Ko nga tauira tuatahi e rua o ia kiko i tangohia e te kete tangohanga-a-roto, a ko nga mea whakamutunga e rua i tangohia e Tguide S16.
Hua Whakamatau: Ko te katoa o te kohinga o te kararehe RNA i tangohia e Tguide S16 ki te reagent 499355 He rite tonu te ma, me te od260 / OD280 huri noa i te 2.0 ~ 2.1 me OD260 / OD230> 2.0. Hei whakamutunga, ka taea te whakamahi i te katoa o te rna i nga kopa kararehe hei whakakapi i te otinga tangohanga-tau-tau.
