TGuide Ataahua Ataahua Tipu DNA Kit
Nama Putumōhio / tākainga
| ngeru. kahore | ID | Nama o preps |
| 4993548(kaata) | DP607-DE | 48 |
He aratohu whiriwhiri reagent mo TGuide S16
| Waikawa Nuclei | Momo Tauira | Ingoa Hua | Rahi kete (whakarite) Kaata/ pereti | ngeru No. Kaata/pereti |
| DNA | Tipa Kararehe | TGuide Smart Tissue Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Te tipu me te kakano | TGuide Ataahua Ataahua Tipu DNA Kit | 48 | 4993548 |
| DNA | Te oneone/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Hua kiri me PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Toto Katoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Pūtau/Swab/Wangi maroke, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Toto/Pūtau/Tipa | TGuide Smart Blood/Cutau/Tipa RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Te tipu me te kakano | TGuide Ataahua Ataahua Tipu RNA Kit | 48 | 4993552 |
| DNA/RNA | Wai Kore Kore Putau (Serum, etc) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Whakataki hua
E tika ana mo nga momo momo kopa tipu, ina koa nga tipu polysaccharide- polyphenol ranei.
Haumaru me te kore paitini: Karekau he reagents waro paitini penei i te phenol/chloroform kei roto.
Te tino parakore: Ko te DNA kua whiwhi he tino parakore, ka taea te whakamahi tika mo te rapu maramara, te raupapatanga teitei me etahi atu whakamatautau.
Ngā āhuatanga
Super ngawari ki te whakamahi
Karekau he mahi paipa atu.
Ka taea te whakatika te rōrahi elution ki te hiahiatia.
Ko te whakaurunga me te mahi he iti noa te whakangungu. Ki nga papatono kua oti te whakauru, wetewete i te kariri, tohua te kawa ka whakahaere i to whakamatautau. Kua reri nga kaupapa ki te whakamahi, ka taea hoki te whakarite.
Ko nga reagents kua oti te utaina me nga taonga pau kua rite.
Ka whakakorehia te whakahaere matū ki te tino nui hei whakarite i te haumaru o te mahi.
Hua Whakaaturanga
Te tangohanga DNA genomic o te riwai
Rahi tauira: 100 mg
Tauira i mua-maimoatanga: huri ki te hauota wai ranei homogenizer kiko
Te kukū o te aporo:2% (TAE)
Te uta i te rōrahi: 2 μl
Tohu: D15000, TIANGEN S16-01, S16-02 me S16-03 e tohu ana i nga whakamatautau whakarara mo te tango i te DNA genomic tipu ma te whakamahi i te 3 TGuide S16 Nucleic Acid Extractors i te wa ano.
He Kamupene: he tohu rongonui
Hua whakamātautau: te whakamahi i te TGuide S16 me te reagent 4993548 ki te tango aunoa i te DNA genomic o nga rīwai kua eke ki te hua pai me te tino parakore, e rite ana ki te kounga hua tangohanga o nga kete tangohanga miro-tīwae whakataetae rongonui. I tenei whakamatautau, tata ki te 11 μg o te waikawa karihi i tangohia mai i te 100 mg o nga puku rīwai, me te OD260/OD280 huri noa i te 1.8~1.9, me te OD260/OD230＞2.0.
Te tangohanga DNA genomic o te rau soybean
Rahi tauira: 100 mg
Tauira i mua-maimoatanga: huri ki te hauota wai ranei homogenizer kiko
Te kutanga o te aporo: 1% (TAE)
Te uta i te rōrahi: 2 μl
Tohu: D15000, TIANGEN
Hua whakamātautau: te whakamahi i te TGuide S16 me te reagent 4993548 ki te tango aunoa i te DNA genomic o nga rau soybean kua tutuki te hua pai me te tino ma. I roto i tenei whakamatautau, tata ki te 13μg o te waikawa karihi i tangohia mai i te 100 mg rau soya, me te OD260/OD280 huri noa i te 1.8~1.9, me te OD260/OD230＞2.0.
Te tangohanga DNA genomic o nga pakiaka witi
Rahi tauira: 100 mg
Tauira i mua-maimoatanga: huri ki te hauota wai ranei kiko huri homogenizer
Te kutanga o te aporo: 1% (TAE)
Te uta i te rōrahi: 2 μl
Tohu: D15000, TIANGEN
He Kamupene: he tohu rongonui
Te hua whakamatautau: te whakamahi i te TGuide S16 me te reagent 4993548 ki te tango aunoa i te DNA genomic o nga pakiaka witi kua tutuki te hua pai me te tino parakore, he rite ki te hua tangohanga me te ma o nga kaiwhakataetae rongonui. I tua atu, ko te pono o te DNA genomic he pai ake i tera o nga hua whakataetae o etahi atu kamupene. I roto i tenei whakamatautau, tata ki te 15 μg o te waikawa karihi i tangohia mai i te 100 mg pakiaka witi, me te OD260/OD280 huri noa i te 1.8~1.9, me te OD260/OD230＞2.0.
