T2T GENOME ASSEMBLY, GAP FREE GENOME
1stGenome vary roa1
Lohateny: Fivoriambe sy Fanamarinana Genome Reference roa tsy misy Gap ho an'ny vary Xian/indica dia manambara ny fomba fijery momba ny Architecture Centromere Plant
Doi:https://doi.org/10.1101/2020.12.24.424073
Navoaka ny fotoana: Janoary 01, 2021.
Institut: Huazhong Agricultural University, Sina
Fitaovana
O. sativa xian/indicakarazana vary 'Zhenshan 97 (ZS97)' sy 'Minghui 63 (MH63)
Strategy sequencing
Mamaky NGS + Mamaky HiFi + Mamaky CLR + BioNano + Hi-C
Data:
ZS97: 8.34 Gb（~23x）HiFi mamaky + 48.39 Gb (~131x) CLR mamaky + 25 Gb(~69x) NGS + 2 BioNano Irys sela
MH63: 37,88 Gb (~103x) HiFi mamaky + 48,97 Gb (~132x) CLR mamaky + 28 Gb(~76x) NGS + 2 BioNano Irys sela
Sary 1 Genome roa tsy misy elanelana amin'ny vary (MH63 sy ZS97)
2ndBanana Genome2
Lohateny: Telomere-to-telomere krômôzôma amin'ny akondro tsy misy elanelana amin'ny alàlan'ny filaharan'ny nanopore
Doi:https://doi.org/10.1101/2021.04.16.440017
Fotoana navoaka: Aprily 17, 2021.
Institut: Université Paris-Saclay, France
Fitaovana
Double haploidMusa acuminatasppmalaccensis(DH-Pahang)
Paikady sy angona momba ny filaharana:
Mode HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ Sarintany Optical (DLE-1+BspQ1)
Tabilao 1 Fampitahana ny fivorian'ny genome Musa acuminata (DH-Pahang).
Figure 2 Musa génomes architecture fampitahana
3rdPhaeodactylum tricornutum génom3
Lohateny: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Navoaka ny fotoana: May 04, 2021
Institiota: Western University, Canada
Fitaovana
Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)
Paikady sy angona momba ny filaharana:
1 Oxford Nanopore minION sela mikoriana + 2 × 75 mitambatra faran'ny afovoany vokatra NextSeq 550 hazakazaka
Figure 3 Workflow for telomere-to-telomere genome assembly
4thGenome CHM13 olombelona4
Lohateny: Ny filaharana feno momba ny génome olombelona
Doi:https://doi.org/10.1101/2021.05.26.445798
Fotoana navoaka: May 27, 2021
Institute: National Institutes of Health (NIH), Etazonia
Fitaovana: cell line CHM13
Paikady sy angona momba ny filaharana:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano sarintany optika, ary Strand-seq
Tabilao 2 Fampitahana ny fivorian'ny genome olombelona GRCh38 sy T2T-CHM13
Reference
1. Sergey Nurk et al. Ny filaharana feno momba ny génome olombelona. bioRxiv 2021.05.26.445798; doi:https://doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al. Telomere-to-telomere gapless chromosomes amin'ny akondro mampiasa nanopore sequencing. bioRxiv 2021.04.16.440017; doi:https://doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al. Telomere-to-telomere genome assembly of Phaeodactylum tricornutum. bioRxiv 2021.05.04.442596; doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al. Ny Fivoriambe sy ny Fanamarinana ny Genome Reference roa tsy misy elanelana ho an'ny vary Xian/indica dia manambara ny fomba fijery momba ny Architecture Centromere Plant. bioRxiv 2020.12.24.424073; doi:https://doi.org/10.1101/2020.12.24.424073
Fotoana fandefasana: Jan-06-2022