Totum transcriptome sequencing - illumina
Features
● Dual Library ad Sequence ad completum Transcriptome: RNNA deperditionem sequitur Pe150 Library praeparatio et magnitudine Electio sequitur by Se50 Library Praeparatio
● completum bioinformatics analysis de mRNA, lncrna, Circrnna, et Mirna in separatum Bioinformatics tradit
● iuncturam analysis omnium RNA expressio in combined fama, inter Cerna networks analysis.
Service commoda
●In-profundum analysis regulatory networks: Cerna network analysis est enabled a iuncturam sequencing de mRNA, lncrna, citcrne, et Mirna et per exhaustaxat bioinformatic workflow.
●Comprehensive annotation: Non utimur multiple databases ad munera annotate differentially expressit genes (guttur) et praestare correspondentes locupletantes analysis, providing insights in cellular et mocecular processibus underlying transcriptome responsum.
●Extensive expertise: Cum a semita record of prospere claudendo super MMC totum transcriptome projects in variis investigationis domain, nostra quadrigis affert divitiis experientia omnibus project.
●Rigorous species imperiumNos effectum deducendi core imperium punctorum per omnes gradus, ex sample et bibliotheca praeparatio ad sequencing et bioinformatics. Hoc methutus vigilantia ensures ad partum constanter summus qualitas praecessi.
●Post-Sales Support: Our commitment extendat ultra project complementum cum III-mensis post-Sale Service tempus. Per hoc tempus, nos offerre project sequantur, sursum, troubleshooting auxilium, et Q & a sessionibus ad oratio quis queries ad eventus
Sample requisitis et partus
| Bibliotheca | Sequencing belli | Notitia commendatur | Imperium |
| RRNA infrequenti | Illumina pe150 | XVI GB | Q30≥85% |
| Magnitudine electus | Illumina se50 | 10-20m legit |
Sample Requirements:
Nucleotides:
| Conc. (NG / μl) | Moles (μg) | Pudicitia | Integritas |
| ≥ LXXX | ≥ 1.6 | OD260 / CCLXXX = 1.7-2.5 OD260 / CCXXX = 0.5-2.5 Limited vel dapibus vel DNA contamination ostensum est in Gel. | Rin≥6.0 5.0≥28s / 18s≥1.0; limited aut non baseline elevatio |
Commendatur Sample partus
Continens: II ml Centrifuge tubo (stagnum ffoyle non commendatur)
Sample Labeling: Group + replicate EG A1 A2, A3; B1, B2, B3.
Shipment:
I. Arida, Ice: Exempla postulo ut facis in sacculos et sepulta in sicco-glacies.
II. Rnastable tubulis: rna samples potest exaruit RNA stabilization tubo (eg rnastable®) et uit ad locus temperatus.
Service opus influunt
Experimentum Design
Sample partum
RNA extraction
Constructio
Sequencing
Analysis
Post-Sale Services
Bioinformatics
RNA expressio Overview
Differentially expressit genes
Cerna Analysis
Differentially expressum mirnas et related rnas
Explore in investigationis progressiones facilitati per BMKGene 'omnis transcriptome sequencing Services per cated collectione publications.
Dai, Y. et al. (MMXXII), 'comprehensive expressio profiles de Mrnas, Lncrnas et Mirnas in Kashin-Becke morbo identified by-sequencing', Molecular omen, XVIII (II), pp. 154-166. Doi: 10.1039 / D1Mo00370D.
Liu, N. Nan et al. (MMXXII), plenus longitudo transcriptomes analysis de frigore resistentia Apis Cerana in Changbai Mountain Per overwintering periodum. ': Gene, DCCCXXX. Pp. 146503-146503. Doi: 10.1016 / J.Gene.2022.146503.
Wang, XJ et al. (MMXXII), Multi-omics integration-fundatur prioritization de competing endogenous RNA moderari networks in parva cellula pulmone cancer: Molecular et medicamento candidati ', in oncology, XII, p. (CMIV) DCCCLXV. Doi: 10,3389 / Fonc.2022.904865 / Bibtex.
Xu P. et al. (MMXXII) 'Analysis in Lncrna / Circna-MRNA expressio profiles reveals novae insights in potentiale mechanisms in responsione ad radix, nodum nematodes in eros', BMC genomics, XXIII (I), pp. 1-12. Doi: 10.1186 / S12864-022-08470-3 / figuras / VII.
Yan, Z. et al. (MMXXII), omnis, transcriptome RNNIQUE EXERCENDIGHTS MOLECULARUM machinationes consociata cum sustentacionem postharvest qualitas in broccoli per rubrum ducitur irradiatio ', postharvest Biology et Technology, CLXXXVIII, p. (CXI) CLXXVIII. Doi: 10.1016 / J.Postarvbio.2022.11878.
