Repraesentatio Bisulfite Reducitur Sequencing (RRBS)
Repraesentatio Bisulfitae Reducta Sequencing (RRBS) emersit ut jocus sumptus efficax et efficax ad Integrum Genome Bisulfite Sequencing (WGBS) in DNA methylation investigationis. Dum WGBS perceptiones comprehensivas praebet, totum genoma in uno basi resolutionis inspiciendo, summus sumptus factor eius limitans esse potest. RRBS opportuna hanc provocationem mitigat, selective dividendo partem repraesentativam genome. Haec methodus methodo CpG insularum opulentarum regionum locupletati innititur MspI bipertito, quam sequitur amplitudo lectionis 200-500/600 bps fragmentorum. Secuntur ergo solae regiones ad insulas CpG proximales, dum insulae CpG distantes ab analysi excluduntur. Hic processus, cum discursu bisulfite coniunctus, permittit ut summus resolutio detectionis methylationis DNA, et accessus sequens PE150, speciatim in fines insertorum potius quam medium intendit, augens methylationis efficientiam proflificationis. Instrumentum inaestimabile est RRBS quod methylationi methylationi gratuitae efficacem sinit inquisitioni ac mechanismi epigeneticae cognitioni proficit.
Features Service
relatio genome requirit.
● Labda DNA ad monitorem bisulfitum conversionis efficientiam adhibetur.
● MspI concoctionem efficacia etiam monitored.
● Duplex enzyme digestio ad exempla plantarum.
Sequentia in Illumina Nova Seq.
Servitium Commoda
●Sumptus efficax et efficax alterna WGBS: ut analysin perficiatur minore sumptu et cum minore exemplo requisita.
●Complete Platform:praebent unum-stop praestantissimum ministerium ab sample processui, bibliothecae constructione, et ad analysin bioinformaticas sequendo.
●Expertise: cum RRBS sequencing incepta feliciter per diversam speciei extensionem peracta, BMKGENE supra decennium experientiae, theam analysin valde peritissimam, contentum comprehensivum, et subsidia post-venditio praeclara.
Service Specifications
| Library | Sequencing Strategy | Commendatur data output | Qualitas imperium |
| MspI digesti et Bisulfite tractatus bibliothecae | Illumina PE150 | 8 Gb | Q30 ≥ 85% Bisulfite conversionem > 99% MspI secans efficientiam > 95% |
Sample Requisita
| Retrahitur (ng/µL) | Summa (μg) |
| |
| Genomic DNA | ≥ 30 | ≥ 1 | Limited degradation vel contagione |
Service Opus O
Sample traditio
Library construction
Sequencing
Analysis Data
Post-venditionis officia
Sequenti analysi includit:
● Rudis qualitas imperium sequendo;
● Mapping ut referat genome;
● Deprehensio 5mC methylatarum basium et identificatio motiva;
● Analysis methylationis distributionis et comparationis specimen;
● Analysis Differentiae Regionum Methylated (DMRs);
● Eget annotationem genesis DMRs adjunxit.
Quality control: digestio efficientiae (in genome mapping)
Quality control: bisulfite conversion (in methylation info extraction)
Methylation map
Sample comparatio: Principal Component Analysis
Aliter Methylated Regionum (DMRs) analysis: heatmap
Explorare progressus investigationis faciliores per BMKGene totum genome bisulfitum sequencing officia per collectionem publicationum curatarum.
Li, Z. et al. (2022) 'Hymni fidelitas reprogrammandi in cellas Leydig sicut per activationem et factorum paracrinarum CRISPR';PNAS Nexus, 1 (4). doi: 10.1093/PNASNEXUS/PGAC179.
Tian, H. et al. (2023) ' Analysis methylationis genome-wide DNA corporis compositionis in gemellis monozygoticis Sinensium';Acta investigationis FusceLIII (11), p. e14055. doi: 10.1111/ECI.14055.
Wu, Y. et al. (2022) 'DNA methylatio et ratio lumborum ad coxam: epigenome-wide studium in geminis monozygoticis Sinensibus';Acta inquisitionis endocrinologicae, 45 (12), pp. doi: 10.1007/S40618-022-01878-4.
