Prokaryotic RNA Sequencing
Features
● RNA Sample dispensando involved RRNA deperditionem sequitur directional RNA Library praeparatio.
● Bioinformatic Analysis secundum alignment ad reference Genome
● Analysis includit gene expressio et gradu sed etiam transcript structuram et SRNA analysis
Service commoda
●Rigorous species imperiumNos effectum deducendi core imperium punctorum per omnes gradus, ex sample et bibliotheca praeparatio ad sequencing et bioinformatics. Hoc methutus vigilantia ensures ad partum constanter summus qualitas praecessi.
●Strand, specifica sequencing notitia: Ob ad RNA Library praeparatio non directional, enabling idem de anti-sensu transcripts.
●Complete Analysis Tailored ad Prokaryotic TranscripomesEt bioinformatic Pipeline includit non solum analysis de gene expressio sed etiam analysis de transcript structuram, comprehendo idem de operons, UTRs et promotores. Etiam includit analysis de SRNAS, nimirum annotationem et praedictionem secundae structuram et peltas.
●Post-Sales Support: Our commitment extendat ultra project complementum cum III-mensis post-Sale Service tempus. Per hoc tempus, ut offerimus project sequantur, sursum, Troubleshooting auxilium, et Q & sessiones ad oratio quis queries ad praecessi.
Sample requisitis et partus
| Bibliotheca | Sequencing belli | Notitia commendatur | Imperium |
| RRNA infrequenti directional Library | Illumina pe150 | 1-2 GB | Q30≥85% |
Sample Requirements:
| Conc. (NG / μl) | Moles (μg) | Pudicitia | Integritas |
| ≥ L | ≥ I | OD260 / CCLXXX = 1.8-2.0 OD260 / CCXXX = 1.0-2.5 Limited vel dapibus vel DNA contamination ostensum est in Gel. | Rin≥6.5 |
Commendatur Sample partus
Continens: II ml Centrifuge tubo (stagnum ffoyle non commendatur)
Sample Labeling: Group + replicate EG A1 A2, A3; B1, B2, B3.
Shipment:
I. Arida, Ice: Exempla postulo ut facis in sacculos et sepulta in sicco-glacies.
II. Rnastable tubulis: RNA exempla potest siccari in RNA stabilization tubo (eg rnastable®) et uit in locus temperatus.
Service opus influunt
Sample partum
Constructio
Sequencing
Analysis
Post-Sale Services
Bioinformatic Analysis Workflow
Includit sequenti analysis:
● rudis data qualis imperium
● Kessinger ad reference
● bibliotheca species taxationem: RNA fragmentation Randomness, Insert Size: et sequencing Saturation
● Functional annotation praedicta coding genes
● expressio Analysis: Correlatione et Principalem Component Analysis (PCA)
● differential gene expressio (gradus)
● Functional annotation et locupletandum
● SRNA Analysis: Predictio, Annotation, scopum et secundarium structuram praedictionem
● Transcript structuram Analysis: Ofterons, incipiens et finis positions, untranslated regionem (uts), promotor et snp / analysis
Saturitatem sequencing
Eget annotation de coding genes
Relatum inter exemplaria
Differentialibus expressit genes (gradus) analysis
Eget locupletantes Analysis
srna annotation
Explorarent progressiones facilitati per BMKGene scriptor Nanopore plena-Longitudo MRNA sequencing Services in hoc Featured publication.
Guan, CP et al. (MMXVIII) 'global transcriptome mutationes Biofilm-formatam Staphylococcus Epidermidis respondens ad totalis alkaloids de Sophorea alopecuroides',Polonica Acta Microbiology, LXVII (II), p. CCXXIII. Doi: 10.21307 / PJM, 2018-024.
